2019
DOI: 10.1128/jvi.01450-18
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Solution Structure, Self-Assembly, and Membrane Interactions of the Matrix Protein from Newcastle Disease Virus at Neutral and Acidic pH

Abstract: Newcastle disease virus (NDV) is an enveloped paramyxovirus. The matrix protein of the virus (M-NDV) has an innate propensity to produce virus-like particles budding from the plasma membrane of the expressing cell without recruiting other viral proteins. The virus predominantly infects the host cell via fusion with the host plasma membrane or, alternatively, can use receptor-mediated endocytic pathways. The question arises as to what are the mechanisms supporting such diversity, especially concerning the assem… Show more

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Cited by 13 publications
(15 citation statements)
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“…Here, we utilized AFM to elucidate structural peculiarities of the full-length IRR in detergent micelles. AFM is widely used to get information on ligand–receptor interactions ( 22 ), protein-membrane interactions ( 23 , 24 ), and a shape of membrane proteins ( 22 ). Unlike cryo-EM or X-ray crystallography, AFM allows studying proteins in their native environment in solution, preserving their fully hydrated and movable state, as well as in lipid environment in nanodiscs ( 23 , 24 , 25 , 26 ).…”
mentioning
confidence: 99%
“…Here, we utilized AFM to elucidate structural peculiarities of the full-length IRR in detergent micelles. AFM is widely used to get information on ligand–receptor interactions ( 22 ), protein-membrane interactions ( 23 , 24 ), and a shape of membrane proteins ( 22 ). Unlike cryo-EM or X-ray crystallography, AFM allows studying proteins in their native environment in solution, preserving their fully hydrated and movable state, as well as in lipid environment in nanodiscs ( 23 , 24 , 25 , 26 ).…”
mentioning
confidence: 99%
“…The boundary potential difference was measured using the inner field compensation method. The method allows the measurement of the transmembrane voltage arising from asymmetric adsorption of charged molecules onto the membrane [26,27,28]. However, IFC cannot give information about the position of the molecule in the membrane.…”
Section: Resultsmentioning
confidence: 99%
“…The boundary potential difference (∆Φ b ) was measured using the inner field compensation (IFC) method, which utilizes the second harmonics of the capacitance current for capacitance minimization [25]. The method allows the measurement of the transmembrane voltage arising from asymmetric (one-side) adsorption of charged molecules onto the membrane [26,27,28] (Figure 2). The boundary potential difference was measured for different bulk concentrations of hydrophobic ions added to one of two compartments of the experimental chamber.…”
Section: Methodsmentioning
confidence: 99%
“…NDV internalization can occur via macropinosomes and trafficking via Rab5a GTPase positive early endosomic vesicles [8]. The transfer of the viral genome from the vesicles to the cytoplasm occurs via membrane fusion [13]. The endocytosis pathway may release the nucleocapsid by accelerating the disintegration of the M proteins scaffold at acidic pH [13].…”
Section: Cellular Infection and Viral Replicationmentioning
confidence: 99%