Solvent extraction selection in the determination of isoflavones in soy foods

The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

“…Similar to Song and Barua 14 , tempeh used in this research was obtained from local market. Since there is no standardization of tempeh production, the ethanolic extract of tempeh was standardized by analyzing the concentration of genistein in the corresponding extract [12][13][14] . Genistein in soy has been accurately analyzed using reverse phase HPLC method [12][13][14]19,26 , which was therefore applied here to determine genistein concentration in the ethanolic extract.…”

“…Tempeh was selected since it contains the highest concentration of genistein 12,14 and as fermented product of soybean it has higher concentration of aglycon isoflavones compared to the seed 14 . The results showed that genistein concentration in the ethanolic extract was 0.681 % (w/w), which wasapproximately 7-folds higher than the total genistein concentration in tempeh, which was extracted for 2 hours using ethanol and dried using evaporator in temperature of less than o C reported by Murphy et al 12 .…”

“…Similar to other phytoestrogens, the isoflavones from soybean have activities in cancer cells resulted from their activity as ligands for estrogen receptors 2,5,6 , a biomarker of breast cancer [7][8][9] .Notably, genistein is the most popular studied isoflavones and has been used as the reference ligand for estrogen receptors, both estrogen receptor a (ERa)and estrogen receptor b (ERb) 10,11 . Tempeh, one of the fermented soy products, has been widely known as a good resource of isoflavones 2,12 . The structures of 12 isoflavones commonly found in tempeh are presented in Figure 1 1, [12][13][14] .…”

“…Tempeh, one of the fermented soy products, has been widely known as a good resource of isoflavones 2,12 . The structures of 12 isoflavones commonly found in tempeh are presented in Figure 1 1, [12][13][14] . Compared to soy flavor and other soyfoods, i.e., soy flavor, tofu, texturized vegetable protein, and soy germ, tempeh contains the highest concentration of genistein 12 .…”

“…The structures of 12 isoflavones commonly found in tempeh are presented in Figure 1 1, [12][13][14] . Compared to soy flavor and other soyfoods, i.e., soy flavor, tofu, texturized vegetable protein, and soy germ, tempeh contains the highest concentration of genistein 12 . The activity of soyfoods in lowering cancer risk of tempeh was also associated with the activity of genistein in inhibiting the activity of tyrosine protein kinase, an overexpressed enzyme in many cancer cells 1,15,16 .…”