Somaclonal variants resistant to sugarcane mosaic virus and their agronomic characterization

Oropeza, Maira; García, Eva de

doi:10.1007/bf02823009

Cited by 13 publications

(9 citation statements)

References 17 publications

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“…The two types of callus obtained were similar to those previously reported for this cultivar (Oropeza, 1994;Oropeza and Garcõ Âa, 1996;De Caires, 1997;Marcano, 1999). The degree of embryogenic callus formation varied depending on the origin of the explant.…”

Section: Discussionsupporting

confidence: 85%

“…A mucilaginous, friable callus and a compact, white callus. Previous studies have demonstrated the non-embryogenic and embryogenic characteristics of these callus types, respectively (Oropeza and Garcõ Âa, 1996). After 7 d of culture, leaf explants from field-grown plants originated non-embryogenic callus, and 2 wk later we observed the formation of embryogenic callus.…”

Section: Resultsmentioning

confidence: 96%

“…Explants obtained from in vitro plants formed more embryogenic callus than explants obtained from field-grown plants. Some authors have reported the production of embryogenic and non-embryogenic calluses from young rolled leaves of in vitro and field-grown cultured sugarcane plants (Ho and Vasil, 1983;Chen and Luthe, 1987;Chen et al, 1988;Guiderdoni and Demarly, 1988;Taylor et al, 1992;Brisibe et al, 1994;Falco et al, 1996;Oropeza and Garcõ Âa, 1996), but no one has compared the callus production between these two types of explants. It has been reported that the degree of embryogenic callus formation varied depending on the cultivar, concentration of 2,4-D tested (Taylor et al, 1992) and on the developmental stage of the leaf (Guiderdony and Demarly, 1988).…”

Section: Discussionmentioning

confidence: 96%

“…Sugarcane tissues from young leaves cultured in vitro in the presence of 2,4-dichlorophenoxyacetic acid (2,4-D) originate two callus types: a white, compact callus (embryogenic) and a friable, mucilaginous callus (non-embryogenic) (Oropeza and Garcõ Âa, 1996). These callus types can be separated to establish embryogenic and non-embryogenic cell suspensions (Colmenares et al, 1998).…”

Section: Introductionmentioning

confidence: 98%

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Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Oropeza

Marcano

García

2001

In Vitro Cell.Dev.Biol.-Plant

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Previous results have shown that some proteins secreted in the culture medium are involved with the formation of embryogenic cells and can modify somatic embryo differentiation. Undifferentiated cell suspensions grown in the presence of 13 mM 2,4-dichlorophenoxyacetic acid (2,4-D) and obtained from embryogenic and non-embryogenic callus were used to study these events in sugarcane plants (cv. PR-62258). The cell suspension growth curves were determined and soluble proteins were extracted from embryogenic and non-embryogenic callus and culture medium from cell suspensions. In embryogenic callus we detected 1.43 times more protein than in non-embryogenic callus and the electrophoretic protein patterns show specific polypeptides for both callus types. In embryogenic callus we detected a cluster of four polypeptides in the range of 38±44 kDa and another polypeptide of 23 kDa that were not observed in non-embryogenic callus. In nonembryogenic callus there is a 35-kDa polypeptide that was not detected in embryogenic callus. In the case of extracellular proteins, the medium from embryogenic cell suspensions contained four polypeptides of 41, 38, 34 and 28 kDa that were slightly detected in the medium from non-embryogenic cell cultures; we also detected a band at 15 kDa that could not be observed in the medium from non-embryogenic cell suspensions. These results suggest that the development of embryogenic callus and cell suspensions is related to the type and amount of intracellular proteins in the callus cells and to the secreted proteins from these cells into the medium.

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Section: Discussionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

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Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Oropeza

Marcano

García

2001

In Vitro Cell.Dev.Biol.-Plant

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“…One possibility is that the two plant populations carry differently virulent SCYLV strains (Abu Ahmad et al 2007), both of which would be detected by PCR analysis. Another possibility is that of somaclonal variation, which has been shown to be high in sugarcane (Heinz et al 1969) and which may affect virus susceptibility (Oropeza and de García 1996). Small genetic variation may suppress viral multiplication in one plant population more than in another.…”

Section: Discussionmentioning

confidence: 98%

RT-PCR and quantitative real-time RT-PCR detection of Sugarcane Yellow Leaf Virus (SCYLV) in symptomatic and asymptomatic plants of Hawaiian sugarcane cultivars and the correlation of SCYLV titre to yield

et al. 2010

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Sugarcane yellow leaf virus (SCYLV) has been reported worldwide to infect sugarcane and to cause significant yield losses. Current detection methods include tissue blot immunoassay (TBIA), reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR assay (qRT-PCR). In this paper, we report the use and comparison of these detection methods for the study of SCYLV in Hawaiian cultivars. We observed positive RT-PCR and qRT-PCR reactions in cultivars previously thought (based on TBIA) to be immune to virus infection. The semi-quantitative virus titre in these cultivars was however at least 10 6 -fold lower than in the cultivars which were known to be SCYLVsusceptible. The RT-PCR methods also revealed that plants of the cultivar H65-7052, which were previously shown to vary strongly between TBIA-positive and TBIA-negative, indeed exhibited fluctuating SCYLV-titres in a range of 10 3 -10 4 -fold. The virus titre was carried through to the next vegetative generation, i. e. plants grown from seed pieces with low virus titre had low virus titre and plants grown from seed pieces with high virus titre contained high virus titre. A small field trial comparing plants of cv. H65-7052 of low and high SCYLV-titre showed that the field plots with plants of high virus titre developed Yellow Leaf symptoms and yielded only 54-60% of cane and sugar tonnage compared to plots with plants of low virus titre.

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Sugarcane Biotechnology: Axenic Culture, Gene Transfer, and Transgene Expression

Birch¹

2013

Sugarcane: Physiology, Biochemistry, and Functional Biology

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Somaclonal variants resistant to sugarcane mosaic virus and their agronomic characterization

Cited by 13 publications

References 17 publications

Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

RT-PCR and quantitative real-time RT-PCR detection of Sugarcane Yellow Leaf Virus (SCYLV) in symptomatic and asymptomatic plants of Hawaiian sugarcane cultivars and the correlation of SCYLV titre to yield

Sugarcane Biotechnology: Axenic Culture, Gene Transfer, and Transgene Expression

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