Somaclonal Variation in Microsperma Lentil (Lens culinaris Medik)

Abstract

Shoot apices of Masoor-85 seedlings (3-4 days old) induced proliferated callus masses under dark conditions, were induced to proliferate callus masses. The callus was induced under dark conditions in MS medium containing K (10 mg/l), GA (1 mg/l) with 5-10 percent lentil seed extract. The shoots were regenerated by transferring the callus culture in light. The shoots were cut and rooted to have plantlets that were finally grown in the field in winter season. These plants were Ro generation. The first selections were made from Ro and seeds were collected. These seeds were grown for R1 generation, whose seeds were selected for R2 generation and so on. Some selected variants were stable till AS generation and were called somaclonal. Variants for more pods and large seed were selected.