Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.)

Kunitake, Hisato; Imamizo, Hideo; Mii, Masahiro

doi:10.1016/0168-9452(93)90239-v

Cited by 58 publications

(20 citation statements)

References 16 publications

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“…This higher efficiency of fructose, compared to sucrose, has been shown in rugosa rose (Rosa rugosa Thunb.) (Kunitake et al 1993) and in somatic embryo development of Picea mariana (Mill.) B.S.P.…”

Section: Discussionmentioning

confidence: 99%

Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Loiseau

Marche

Deunff

1995

Plant Cell Tiss Organ Cult

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Studies were conducted to test the effects of various auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis from shoot apices of pea (Pisum sativum L.) cultured on a sole medium. Picloram (4.5 #M) and 4-chlorophenoxyacetic acid (45 #M) were the most effective auxins. Addition of cytokinins (benzyladenine, zeatin, kinetin) to auxin-containing medium reduced embryo production. Amino acids (glutamine, alanine, proline) did not improve somatic embryogenesis. Carbohydrate seemed to be a critical factor. Embryogenic efficiency and embryo development were promoted by high carbohydrate concentration. The best results were obtained with fructose (252-504 mM); the number of somatic embryos per cultured explant was 3-to 4-fold higher compared to the control (84 mM sucrose). From these results, an optimized induction medium is proposed.

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Section: Discussionmentioning

confidence: 99%

Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Loiseau

Marche

Deunff

1995

Plant Cell Tiss Organ Cult

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“…Although, there are a few reports on regeneration of shoots and/or plantlets from zygotic embryos and somatic tissues of various Rosa species (Lloyd et al, 1988;Burger et al, 1990;De Wit et al, 1990;Rout et al, 1992;Kunitake et al, 1993), regeneration of plantlets via somatic embryogenesis from somatic tissues of Rosa hybrida cultivars remains difficult with a low frequency of occurrence (Rout et al, 1991;Noriega & Sondahl, 1991). Embryogenic callus initiated from immature leaf and stem segments of R. hybrida cv.…”

Section: Introductionmentioning

confidence: 99%

Organogenesis and somatic embryogenesis in callus cultures ofRosa hybrida andRosa chinensis minima

Hsia

Korban

1996

Plant Cell Tiss Organ Cult

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Pre-incubation of somatic tissues of the cut rose 'Carefree Beauty' and miniature roses 'Red Sunblaze' and 'Baby Katie' in 10, 100, or 200 #M 2,4-D induced the development of highly rhizogenic callus. Upon transfer ofrhizogenic callus to a regeneration medium containing 23 #M TDZ and 3 #M GA3, a low frequency of shoot organogenesis (3.3%) and somatic embryogenesis (6.6%) was observed. Incubation of leaf and stem internodes in 11, 27, 54, 81, or 108/zM NAA followed by transfer of explants to the regeneration medium resulted in up to a 25% increase in shoot organogenesis from callus-derived internodal explants of 'Red Sunblaze', but no somatic embryogenesis was observed. The influence of glucose versus sucrose in the regeneration medium on leaf explants of 'Carefree Beauty' and 'Baby Katie' pre-incubated in 100 #M 2,4-D revealed a difference in genotypic response to shoot organogenesis and somatic embryogenesis. For 'Carefree Beauty', a concentration of 111 mM glucose induced higher frequencies of both organogenic (33%) and embryogenic calluses (25%) than either 59 mM or 117 mM sucrose. For 'Baby Katie', no significant difference was found for number of organogenic calluses induced on 59 mM sucrose and 111 mM glucose.

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“…Regeneration from somatic embryos was first reported by (Souq et al, 1996) from calli of leaf, filaments and stamens. Subsequently, it was also reported from calli of leaf; filaments and immature seeds (Kunitake et al, 1993).…”

Section: In Vitro Micropropagation Of Rose Plantmentioning

confidence: 97%

Genetic Transformation Protocol for Early Flowering Cryptochrome Gene in Taif Rose Plant

Asaal¹,

shahaby²

2017

Int.J.Curr.Microbiol.App.Sci

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Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.)

Cited by 58 publications

References 16 publications

Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Organogenesis and somatic embryogenesis in callus cultures ofRosa hybrida andRosa chinensis minima

Genetic Transformation Protocol for Early Flowering Cryptochrome Gene in Taif Rose Plant

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