Somatic Embryogenesis in Garlic (Allium Sativum L.)

Keleş, Davut; Taşkin, Hatıra; Baktemur, Gökhan; Yücel, Namık Kemal; Büyükalaca, Saadet

doi:10.17660/actahortic.2011.923.4

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“…Plant regeneration by somatic embryogenesis is a rare phenomenon in garlic [26]. Optimization of media composition is necessary to ensure better yield and quality of somatic embryos in garlic [27].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, successful regeneration depends on the genotype, the type of explant and its physiological conditions, and the combination of growth regulators in the medium [28]. Several reports have presented embryogenesis in garlic tissue culture [26,[29][30][31][32][33] by optimising the media components and the type of explant. To the best of our knowledge, there are no reports on the regeneration of virus-free garlic plants by somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Production of Virus-Free Garlic Plants through Somatic Embryogenesis

et al. 2021

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The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (¼ of KNO3 and NH4NO3 and 2xMgSO4) containing 0.1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L−1 2,4-D + 0.5 mg L−1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L−1 2,4-D and rooted in a medium containing 0.05 mg L−1 1-naphthaleneacetic acid (NAA) + 0.005 mg L−1 6-(γ,γ-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L−1 2,4-D than those developed in media containing 1 mg L−1 2,4-D + 0.5 mg L−1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods.

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Section: Introductionmentioning

confidence: 99%