Somatic Embryogenesis in Ginseng (Panax Species)

Shoyama, Yukihiro; Zhu, Xuan Xuan; Matsushita, Hirohisa; Kishira, H.

doi:10.1007/978-3-642-78643-3_28

Cited by 18 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mature torpedo-stage embryos as well as immature somatic embryos after longterm maintenance developed into plantlets lacking roots on the same media (Table 1) and their transfer to ex vitro conditions was not successful. Similar results were observed for example by Arya et al (1993), Shoyama et al (1995), Kevers et al (2002) and Choi et al (1998).…”

Section: Resultssupporting

confidence: 91%

“…Tang (2000) reported morphogenic callus to maintain its embryogenic potential over 12 months only and Shoyama et al (1995) reported a gradual decrease in embryo propagation ability and regeneration efficiency after 2 years of subculture. Also culture hyperhydricity, usually related to long-term cultivation, causes regeneration of plants with abnormal morphological appearance and physiological functions (Debergh et al 1992).…”

Section: Introductionmentioning

confidence: 96%

“…Nevertheless, in these cases, somatic embryos are frequently reported to be regenerated into multiple shoots that either miss a root system (Butenko et al 1968;Chang and Hsing 1980;Choi 1988;Č eliµrovµ et al 1992) or have an inadequate or poor root formation (e.g. Arya et al 1993;Shoyama et al 1995;Kevers et al 2002). Choi et al (1998) suggest that the inability of regenerated plants to form roots might be related to embryonic structural abnormalities since morphologically abnormal somatic embryos (e.g.…”

Section: Introductionmentioning

confidence: 97%

“…Nowadays, wild ginseng is rarely available and the current supply of ginseng on the markets mainly depends on field cultivation, which is an extremely long-lasting and laborintensive process. Native ginseng plants need 5-7 years prior to harvest and the content of ginsenosides is low (Shoyama et al 1995). One of the most critical steps in field cultivation is seed germination.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

2004

View full text Add to dashboard Cite

Embryogenic culture was initiated from mature zygotic embryos of Panax ginseng. Multiple somatic embryos formed and proliferated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2.26 microM) and kinetin (0.046 microM). Mature as well as immature somatic embryos grew into plantlets lacking roots on the same media. Histomorphological analysis of somatic embryos treated with abscisic acid (ABA) and polyethylene glycol (PEG 4000) showed a slight improvement in the root meristem organization of torpedo-stage embryos (embryos were more compact and their cells exhibited a lower degree of vacuolation). Shoot regeneration of non-treated somatic embryos was 31% while that for somatic embryos treated with PEG 4000 and ABA was 70%. Moreover, 75% of plants regenerated from PEG- and ABA-treated embryos formed roots while plants from non-treated embryos did not form roots.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

2004

View full text Add to dashboard Cite

show abstract

“…6). Commercial micropropagation and application of biotechnology for such plants are stymied unless the embryogenic/ organogenic strategy can be successfully employed (Shoyama et al, 1995;Tirajoh et al, 1998).…”

Section: Problems In Establishing Shoot Culturesmentioning

confidence: 99%

Special symposium: In vitro plant recalcitrance recalcitrance of woody and herbaceous perennial plants: Dealing with genetic predeterminism

McCown

2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

As a general group, long-lived perennial plants probably present the most challenging obstacles to the researcher, breeder or propagator utilizing microculture as a tool. These challenges appear during all stages of the microculture process, but are probably most resplendent during the stabilization phase. What may be particularly frustrating is that much of this 'recalcitrance' is genetically driven and is thus difficult to control by environmental and nutritional manipulations in microculture. Perennials have complex seasonal cycles and life cycles, which complicate control of their growth in microculture. As shoot cultures have provided useful tools for overcoming these limitations, the inability to establish stabilized shoot cultures is a major form of recalcitrance. Plants having seasonal growth dynamics dominated by strong episodic or determinant shoot growth are some of the most recalcitrant species because stabilized shoot cultures cannot be readily generated. In some cases, episodic growth may be tied closely to phase state and can thus be controlled by manipulating phase; nevertheless, adequate controls have not been identified for many problematic plants. Another trait contributing to recalcitrance of perennials is the relatively slow growth rate in microculture. Slow growth complicates such procedures as selection of transformed tissues. The high phenolic content of many perennial tissues can interfere with the efficacy of transgenic traits such as b-glucuronidase. Developmentally determined growth characteristics such as plagiotropism may persist through all stages of microculture and complicate the recovery of commercially useful micropropagules. Although some technical approaches can occasionally circumvent immediate microculture limitations, general solutions await the development of a deeper understanding of physiological bases of such genetically predetermined phenomena.

show abstract

Cryopreservation of Panax (Ginseng)

Yoshimatsu

Shimomura

2002

Biotechnology in Agriculture and Forestry

View full text Add to dashboard Cite

Somatic Embryogenesis in Ginseng (Panax Species)

Cited by 18 publications

References 12 publications

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos

Special symposium: In vitro plant recalcitrance recalcitrance of woody and herbaceous perennial plants: Dealing with genetic predeterminism

Cryopreservation of Panax (Ginseng)

Contact Info

Product

Resources

About