Somatic Embryogenesis in Herbaceous Dicots

KrishnaRaj, Sankaran; Vasil, Indra K.

doi:10.1007/978-94-011-0485-2_10

Cited by 59 publications

(18 citation statements)

References 368 publications

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“…The induction of embryogenic callus is in line with many reports indicating that 2, 4-D alone or in combination with lower level of cytokinins are effective in inducing embryogenic callus for many plant species (Brown et al, 1995). However, induction of embryogenic callus did not automatically lead to regeneration of shoots, using lower concentrations of BAP.…”

Section: Discussionsupporting

confidence: 71%

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

Asande¹,

Indieka²,

Adero³

et al. 2016

Afr. Crop Sci. J.

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Section: Discussionsupporting

confidence: 71%

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

Asande¹,

Indieka²,

Adero³

et al. 2016

Afr. Crop Sci. J.

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“…Since then, it has been studied and described in many important species (reviews by Bajaj 1995;Brown et al 1995) but the presence (or absence) of differentiating structures has often been overlooked or not emphasized. Numerous early reports of SE can be cited in which the reproducibility/authenticity was not considered or evaluated, particularly in plant species which had previously been considered to be recalcitrant or very difficult to evoke a SE response.…”

Section: Introductionmentioning

confidence: 98%

Somatic embryogenesis in Solanum tuberosum L.: a histological examination of key developmental stages

Sharma¹,

Millam²

2004

Plant Cell Rep

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A potential novel method of producing high-quality potato ( Solanum tuberosum L.) nuclear seeds is through the process of somatic embryogenesis (SE). Somatic embryo formation has been successfully reported in many plant species, but in potato, reliable SE systems are still at the experimental stage. A key factor in the success of any SE system is the ability to discriminate SE-specific cellular structures from those emerging through an organogenic route. In the investigation reported here we attempted to discriminate the progression of specific stages of potato SE by histological means. Internodal segment (INS) explants from 4- to 6-week-old cv. Desiree in vitro cultures were successively cultured on SE induction (for 2 weeks) and expression/regeneration media (for 3 weeks) with and without 2,4-dichlorophenoxyacetic acid (5 microM). Microscopic examination of histological slides prepared using INS explants at different stages revealed the presence of characteristic globular, heart and torpedo stages in the potato SE system along with other associated unique features such as protoderm development and discrete vascular connections. These results confirm the occurrence of potato SE as per the accepted definition of the term.

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“…Culture systems for inducing embryogenesis have been developed for many angiosperm and gymnosperm species (Brown et al 1995;Dunstan et al 1995;Krishnaraj and Vasil 1995;Thorpe and Stasolla 2001). To date, the only conclusive evidence on this subject is that a cell culture has to be grown in a medium containing a high concentration of auxin in order to become embryogenic.…”

Section: Introductionmentioning

confidence: 99%

Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.

Leljak-Levanić

Bauer

Mihaljević³

et al. 2004

Plant Cell Rep

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Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2,4-D and on auxin-free medium supplemented with 1.0 m M NH(4)Cl. DNA methylation was correlated with early embryo development in a manner that was not exclusively dependent on the presence/absence of exogenous auxin. DNA methylation decreased during embryo maturation on auxin-free MSC medium and on auxin-free MSC supplemented with 12.3 micro M 5-azacytidine (5-azaC). The embryogenic features of the pumpkin tissue were preserved, even after a 2-month treatment with 5-azaC.

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Somatic Embryogenesis in Herbaceous Dicots

Cited by 59 publications

References 368 publications

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

Somatic embryogenesis in Solanum tuberosum L.: a histological examination of key developmental stages

Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.

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