Somatic embryogenesis in leaf callus from a mature Quercus suber L. Tree

Pinto, Glória; Valentim, Helena Virgínia Topa; Costa, Armando; Castro, Sílvia; Santos, Conceição

doi:10.1079/ivp2002352

Cited by 37 publications

(44 citation statements)

References 11 publications

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“…In the case of somatic embryogenesis based on leaves of C. sativa, it has been observed that the embryonic response is closely associated with nodular calli (Corredoira et al, 2003); although Giovanelli et al (2004) also associated the morphogenic response, expressed in adventitious shoots, with nodular areas in chestnut cotyledons. In calli from leaves of Quercus suber L., friable calli do not present a morphogenic response, which is only evidenced in callus with a compact aspect (Pinto et al, 2002). In the present study, the calli produced in different culture medium, while they presented differences in terms of consistency and color, did not show observable morphogenic responses at the macromorphological level.…”

Section: Resultscontrasting

confidence: 56%

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Avilés¹,

Ríos²,

González³

et al. 2009

Chilean J. Agric. Res.

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To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm 2 , previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.

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Section: Resultscontrasting

confidence: 56%

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Avilés¹,

Ríos²,

González³

et al. 2009

Chilean J. Agric. Res.

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show abstract

“…We also found that the protocol developed by Pinto [22] combined a simple methodology with a low-hormone method reducing the risk of somaclonal variation. Our results strongly indicated that all the samples analyzed and no plantlets are regenerated except that some adventitious buds have formed [7].…”

Section: Discussionmentioning

confidence: 73%

High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

et al. 2017

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Amorphophallus konjac (Araceae) a perennial herb, it has high medicinal and industrial value. In this study, a simple and efficient system for direct somatic embryogenesis and plantlet regeneration of Amorphophallus konjac was developed. The floral axis was used as the experimental material. The primary callus, developed from the floral axis grown on Murashige and Skoog (MS) medium supplemented with different hormone combination at different concentrations. The highest rate of embryogenic callus formation was observed on the MS medium containing 9.04 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 5.37 µM naphthalene acetic acid (NAA). The maximum induction rate was 79.8%, and the embryogenic calli were able to subculture on a medium containing similar hormone combination for over 1 year. The calli were also placed on different media for regeneration and it produced complete plants with shoots and root systems simultaneously. The highest differentiation rate of the embryogenic calli grown on differentiation medium supplemented with 8.88 µM 6-benzylaminopurine (6-BA) and 5.37 µM NAA was 95.6%. Flow cytometry analysis showed no ploidy variation in all the regenerate plantlets.

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“…Somatic embryogenesis offers advantages in improving forest species over other in vitro propagation methods (Park, 2002), namely: a) somatic embryos simultaneously possess both the shoot and root meristems; so a distinct rooting stage, which usually involves stressing procedures, is not necessary; b) during somatic embryogenesis, embryos/clusters are often formed faster and potentially at extremely high numbers per explant; c) somatic embryogenesis is amenable to automation, which means it may become cheaper than other clonal propagation techniques (Pinto et al, 2002;2008); d) finally, a robust and efficient protocol of somatic embryogenesis will allow that embryogenic clonal lines can be preserved for long periods (e.g., in liquid N 2 ) while corresponding plants are transferred to field conditions and are monitored for their characteristics.…”

Section: General Conceptsmentioning

confidence: 99%

“…In particular, cork oak is an abundant species in the Atlantic and West Mediterranean countries where it is an important component of Mediterranean ecosystems (Pinto et al, 2002). Cork is the bark of the oak, which is a natural, renewable and sustainable raw material product of economic interest for a range of applications.…”

Section: Quercus Suber In Vitro Cloning: a Reliable Protocol?mentioning

confidence: 99%

“…In particular, the currently available rates of success in cork oak plant regeneration by somatic embryogenesis Lopes et al, 2006;Loureiro et al, 2005;Pinto et al, 2001;2002) and in cork oak material cryopreservation (Fernandes et al, 2008;Fernandes, 2011) are highly encouraging for researchers and breeders to consider the integration of these strategies in breeding and conservation programs of this species (Figure 1). …”

Section: Quercus Suber In Vitro Cloning: a Reliable Protocol?mentioning

confidence: 99%

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Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Santos¹

2012

Current Frontiers in Cryopreservation

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Somatic embryogenesis in leaf callus from a mature Quercus suber L. Tree

Cited by 37 publications

References 11 publications

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

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