Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Capelo, Ana; Silva, Sónia; Brito, Gina; Santos, Conceição

doi:10.1007/s11240-010-9773-x

Cited by 35 publications

(29 citation statements)

References 29 publications

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“…However, 4-CPA has been successfully used in other species such as Pisum sativum (Loiseau et al 1995), Rosa sp. Auxins are involved in regulating the cell cycle and promoting cell differentiation and play important roles in somatic embryogenesis in several species (Jiménez 2005, Kurczynska et al 2007, Capelo et al 2011, Rocha et al 2012). …”

Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

“…The genotype may influence the variation of embryogenic responses during the induction and maturation phase in plant tissue (Bell et al 1993). In addition, factors such as the type and concentration of auxin (Anandan et al 2012), the type of explant (Capelo et al 2011), presence or absence of abscisic acid (Bell et al 1993), activated carbon (López-Pérez et al 2005) or osmotic substances (Heringer et al 2013) can alter the responses among genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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“…Auxins and cytokinin are the main plant growth regulators involved in the regulation of plant cell cycling, division and differentiation, playing key roles in somatic embryogenesis of many species (Fehér et al 2003;Jiménez 2005;Capelo et al 2011;Cerezo et al 2011;Parimalan et al 2011;Paul et al 2011;You et al 2011).…”

Section: Embryogenic Callus Inductionmentioning

confidence: 99%

Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes

Pinto

Almeida

Rêgo

et al. 2011

Plant Cell Tiss Organ Cult

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Mature zygotic embryos of three genotypes of Passiflora edulis Sims, including 'FB-100', 'FB-200', and 'FB-300' were incubated on a Murashige and Skoog (MS) (1962) medium supplemented with different concentrations (18.1-114.8 lM) of 2,4-diclorophenoxyacetic acid (2,4-D) and 4.4 lM of 6-benzyladenine (BA). MS basal medium and MS with BA induced germination of P. edulis embryos. The highest frequencies of embryogenic calli were observed when explants were incubated on MS medium supplemented with 72.4 lM 2,4-D and 4.4 lM BA for 'FB-200', which showed the highest potential for embryogenic callus formation. Cytological and histological analyses of pro-embryogenic callus revealed two distinct cell types: thin-walled, small, isodiametric cells with large nuclei and dense cytoplasm, typical of intense metabolic activity; and elongated and vacuolated cells, with small nuclei and less dense cytoplasm. Differentiation of somatic embryos was promoted on MS medium supplemented with activated charcoal and indole-3-acetyl-L-aspartic acid (IAA-Asp) either with or without 2,4-D. However, no conversion of somatic embryos into plantlets was observed.

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“…Furthermore, SE has become a widely used technique in genetic transformation and mass propagation of elite genotypes (Table 1). Light and electron microscopic studies have reported on the cellular origin of somatic embryos during primary (Blazquez et al 2009;Capelo et al 2010;Lin et al 2011;Parra-Vega et al 2013) and repetitive/secondary embryogenesis (Dai et al 2011;Pavlović et al 2013;Raju et al 2013). Upon exposure to SE induction medium, the initial events signifying cytological changes in the formation of somatic embryos were evident in 4-6 days (Canhoto et al 1996;Kurczyńska et al 2007).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Insights into the multifaceted application of microscopic techniques in plant tissue culture systems

Moyo

Aremu

Staden

2015

Planta

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Microscopic techniques remain an integral tool which has allowed for the better understanding and manipulation of in vitro plant culture systems. The recent advancements will inevitably help to unlock the long-standing mysteries of fundamental biological mechanisms of plant cells. Beyond the classical applications in micropropagation aimed at the conservation of endangered and elite commercial genotypes, plant cell, tissue and organ cultures have become a platform for elucidating a myriad of fundamental physiological and developmental processes. In conjunction with microscopic techniques, in vitro culture technology has been at the centre of important breakthroughs in plant growth and development. Applications of microscopy and plant tissue culture have included elucidation of growth and development processes, detection of in vitro-induced physiological disorders as well as subcellular localization using fluorescent protein probes. Light and electron microscopy have been widely used in confirming the bipolarity of somatic embryos during somatic embryogenesis. The technique highlights basic anatomical, structural and histological evidence for in vitro-induced physiological disorders during plant growth and development. In this review, we discuss some significant biological insights in plant growth and development, breakthroughs and limitations of various microscopic applications and the exciting possibilities offered by emergent in vivo live imaging and fluorescent protein engineering technologies.

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Somatic embryogenesis induction in leaves and petioles of a mature wild olive

Cited by 35 publications

References 29 publications

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes

Insights into the multifaceted application of microscopic techniques in plant tissue culture systems

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