Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding

Schilde-Rentschler, L.; Boos, G. V.; Ninnemann, Helga

doi:10.1007/978-94-009-2788-9_66

Cited by 9 publications

(2 citation statements)

References 4 publications

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“…Alginate has been reported to prevent the agglutination of protoplasts, support cell wall reconstruction, and promote mitotic division by reducing polyphenol production [ 41 ]. The stimulating effect of embedding protoplasts in sodium alginate has been demonstrated for many plant species, including apple [ 43 ], potato [ 44 ], and Arabidopsis thaliana [ 45 ]. The researchers of most of these studies used liquid culture for alginate-embedded protoplasts.…”

Section: Discussionmentioning

confidence: 99%

A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

Moon

Park

et al. 2021

Plants

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Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.

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Section: Discussionmentioning

confidence: 99%

A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

Moon

Park

et al. 2021

Plants

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“…The unique properties of ribosomal DNA have been exploited both in population genetic studies as well as in phylogenetic analysis of higher plants. RFLP analysis using rDNA as a probe became a routine method for characterization of dihaploid breeding lines (Gruber 199 1 ) or somatic hybrids obtained by protoplast fusion (Hemleben et al 1992), a technique that is widely used in potato breeding programs (Wenzel et al 1979;Schilde-Rentschler et al 1987). Therefore, the information on the molecular organization of rDNA in different potato species has applied relevance.…”

Section: Introductionmentioning

confidence: 99%

Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae

Borisjuk¹,

Borisjuk²,

Petjuch³

et al. 1994

Genome

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The organization of the nuclear-encoded 18S, 5.8S, and 25S ribosomal RNA genes (ribosomal DNA; rDNA) of 21 New World species from different sections of the genus Solanum, of two Old World Solanum species, and of representatives of other Solanaceae (Nicotiana, Atropa, Datura, Physalis, and Capsicum) was analyzed by restriction enzyme mapping using different rDNA specific hybridization probes. All Solanum species investigated exhibited rDNA repeats between 8.7 and 9.3 kb in length; the only exception was S. neorossii with a repeat length of 10.3 kb. Sequence heterogeneity was observed mostly in the intergenic spacer (IGS) region. Restriction sites for EcoRI and DraI in the spacer sequences were found to be characteristic for the New World species of the genus Solanum and for Lycopersicon esculentum. An additional XbaI site was detected in the spacer region of two nontuber-bearing species, S. brevidens and S. etuberosum (subsection Estolonifera Hawkes; series Etuberosa), as well as in the primitive tuber-bearing species of the series Pinnatisecta and Polyadenia (subsection Potatoe G. Don), thus demonstrating that these Mexican species are separated from the other tuber-bearing species but are closely linked to the nontuber-bearing Estolonifera group. Two EcoRI sites mapped at the 3' end of the 25S rRNA coding region seem to be characteristic for members of the Solanaceae; the first EcoRI site is apparently methylated in approximately 50% of the rDNA repeats. Southern hybridization with an IGS fragment of Solanum tuberosum as hybridization probe and nucleotide sequence analysis of the phylogenetically informative 3' end of the 25S rDNA support the assumption that the New World species of the genus Solanum are closely related to Lycopersicon (tomato) in contrast with other Solanaceae investigated, Nicotiana, Atropa, Datura, Physalis, and Capsicum.(ABSTRACT TRUNCATED AT 250 WORDS)

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Breakthrough in the Plant Regeneration from Protoplasts of Vicia Faba and V. Narbonensis

Tegeder

Schieder

Pickardt

1995

Current Issues in Plant Molecular and Cellular Biology

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Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding

Cited by 9 publications

References 4 publications

A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae

Breakthrough in the Plant Regeneration from Protoplasts of Vicia Faba and V. Narbonensis

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