1961
DOI: 10.1139/b61-127
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Somatic Mitosis in a Basidiomycete

Abstract: The nuclei in stained preparations of the mycelium of an unidentified basidiomycete were shown to divide by mitosis. Typical stages of division with the esception of early prophase were clearly distinguished and the haploid chromosome number was shown to be 4. The view that the fungus is a basidiomycete was confirmed by the demonstration of conjugate nuclei and clamp connections.' Manuscript received March 31, 1961. Contribution No.

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Cited by 26 publications
(3 citation statements)
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“…Washed conidial pellet samples from each treatment were resuspended in 5 ml of Carnoy's fluid (75:25, vlv, of absolute ethanol and glacial acetic acid, respectively). Drops of conidial suspensions were placed on glass slides coated with 0.1 % carrageenan and, after 24 h, stained with Giemsa-HCl dye (28). Coverslips were affixed with Permount adhesive (Fisher Scientific, Pittsburgh, PA).…”
Section: Methodsmentioning
confidence: 99%
“…Washed conidial pellet samples from each treatment were resuspended in 5 ml of Carnoy's fluid (75:25, vlv, of absolute ethanol and glacial acetic acid, respectively). Drops of conidial suspensions were placed on glass slides coated with 0.1 % carrageenan and, after 24 h, stained with Giemsa-HCl dye (28). Coverslips were affixed with Permount adhesive (Fisher Scientific, Pittsburgh, PA).…”
Section: Methodsmentioning
confidence: 99%
“…At the London Research Centre of Agriculture Canada, Edmund W.B. Ward surprised many mycologists with his assertion that conventional mitosis took place in Basidiomycetes (161). This was at a time when the predominant view was that vegetative nuclear division was amitotic.…”
mentioning
confidence: 99%
“…A variety ofcytological stains and staining procedures including Giemsa-HCl (23), acetic-orcein (11), propionic-carmine (17), iron haematoxylin (20), safranin 0 (1), aniline blue or trypan blue (5), toluidine blue (9), and basic fuchsin or Feulgen stain (4,16) have been used to investigate the nuclear condition of reproductive and somatic structures of fungi. Fluorescent stains such as acriflavin (19), acridine orange (22), 2,5-bis(4-aminophenyl)-1 ,3,4-oxadiazole (BAO) (14), and especially 4,6-diamidino-2-phenylindole (DAPI) (3,13,18) have been employed more recently to differentiate and improve the resolution of nuclear DNA from other cellular constituents and to quantitate genomic DNA through microspectrophotometric analyses.…”
mentioning
confidence: 99%