Somatic polyploidy and its consequences for flower coloration and flower morphology in azalea

Schepper, Stefanie De; Leus, Leen; Mertens, M.; Debergh, Pierre; Bockstaele, Erik Van; Loose, Marc De

doi:10.1007/s002990100372

Cited by 28 publications

(19 citation statements)

References 33 publications

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“…Also, differently colored leaf margins are significantly wider with brighter coloring. This is similar to what was found for ploidy chimeras of Azalea (De Schepper et al 2001). Flowers of fully tetraploid hostas customarily are slightly larger and frequently more upright facing than partially tetraploid hostas.…”

Section: Introductionsupporting

confidence: 86%

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Sports and hybrids of triploid Hosta ‘Sum and Substance’ reveal chromosome losses and gains in all three apical layers

Zonneveld

Pollock²

2012

Plant Syst Evol

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Ploidy distributions in L1, L2, and L3 apical or meristematic layers of 56 different plants (79 accessions) from vegetative and sexual progeny of the triploid Hosta 'Sum and Substance' were determined. Nuclear DNA contents (2C) of each apical layer were measured by flow cytometry with propidium iodide, and inferred ploidies are calculated. During tissue culture, the triploid (L1-L2-L3 = 3-3-3) Hosta 'Sum and Substance' exhibited chromosome losses resulting in somaclonal variants such as DNA aneuploids (e.g., 2.7-2.7-2.7) and aneuploidy chimeras (e.g., 3-2.7-2.7). Most interestingly, some chimeras exhibited even an increase in genome size as in plants with 3.5-3-3 configuration. Hybrids of H. 'Sum and Substance' show only losses of nuclear DNA compared with the original triploid. This gives rise to fully aneuploid plants and no chimeras. The measurements of Hosta 'Sum and Substance' lineages of sports and hybrids indicate that chromosome losses or gains are an important source of new cultivars. The complexity of chromosomal distribution in derivatives from the triploid Hosta 'Sum and Substance' is discussed.

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Section: Introductionsupporting

confidence: 86%

“…The exception is H. 'Eagle's Nest', an amazing hypertetraploid that gained DNA in L3 with L1-L2-L3 = 3-3-4.3. Such losses and gains might be due to the triploidy of 'Sum and Substance', aggravated by unnatural circumstances in tissue culture, leading to unbalanced mitoses (De Schepper et al 2001).…”

Section: Hosta 'Sum and Substance'mentioning

confidence: 99%

Sports and hybrids of triploid Hosta ‘Sum and Substance’ reveal chromosome losses and gains in all three apical layers

Zonneveld

Pollock²

2012

Plant Syst Evol

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“…One of the immediate and obvious consequences of polyploidy in plants is an increase in cell size which in turn leads to enlarged plant organs, a phenomenon termed gigas effect (Acquaah, 2007;Levin, 2002). For example, the volume of tetraploid cells usually is about twice that of their diploid progenitors (Acquaah, 2007;Emsweller and Ruttle, 1941;Levi et al,2002;Schepper et al, 2001).As the enlargement of the stomata in this observation point out the most of the cholchicine treated polyploid plant production compare with the diploids, the length and width of guard cells increased by 48.25% and 52.62%, respectively in previous studies (Przywara et al, 1988;Cohen and Yao 1996;Pansuksan et al, 2014;Quin et al, 2016).…”

Section: Acclimatization and Transferring Of Plantssupporting

confidence: 54%

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

Perera

Dahanayake

Senanayake

et al. 2016

Trop Agric Res & Ext

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Exacum ritigalensis (Gentianaceae) (Sinhalese: Binara or Ginihiriya) has a potential to develop as a potted plant by improving the size of the flowers and inflorescence. The aim of the study was to establish an efficient and effective system for the production of tetraploid plants through colchicine treatment. Single nodal cuttings (0.5 cm) from aseptic plantlets were established on Murashige and Skoog basal medium (MS) supplemented with 2 mgL -1 BAP and different colchicine concentrations (0, 10, 20, 30, 40 and 50 mg -1 ). They were kept in different durations (0, 7, 14 21 and 28 days) and transferred into 10ml of MS basal medium containing 2 mgL -1 BAP. After one month, they were transferred to MS medium containing 2mgL -1 Indole-3-butyric acid (IBA) for rooting. Then root tips were collected from treated plants to observe chromosome number. Afterwards the selected diploid and tetraploid plants were acclimatized and transferred to field to observe the morphological characters. Factorial Complete Randomized Design (FCRD) with 10 replicates was used for each treatment and statistical analysis was carried out using Duncan's Multiple Range Test of SAS program (9.1.3).The highest percentage of tetraploid plantlets (26.6%) was observed from nodal cuttings treated with MS basal medium with 2mgL -1 BAP + 30mgL -1 colchicine for seven days. The chromosome number of diploid and tetraploid E. ritigalensis determined as 28 and 56 respectively. Cells of the tetraploid plants were comparatively larger cells than diploids plants. The stomata size of the tetraploid (2.96*2.27 μm 2 ) were larger than those of the diploids (1.94*1.48μm 2 ). Leaf shape of diploids was lanceolate while it was ovate or narrowly elliptic in tetraploids. Flower petals of diploid plants were overlapping along their entire length while in tetraploids flower petals were not overlapping. The plants height was significantly higher in diploid (67.00±9 cm) than in tetraploid (38.00±4 cm). Newly produced polyploids of E. ritigalensis have a potential to popularize as a potted plant in the floriculture trade.

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“…Cytometric studies were conducted in Rhododendron spe cies and cultivars (De Schepper et al 2001, Jones et al 2007, Lattier et al 2013. Availability of these data are important not only for plant breeding but also for various fields of ba sic research, including rhododendron taxonomy and evolu tionary changes (Chahal & Gosal 2002, Contreras et al 2007.…”

Section: Flow Cytometric Determination Of Genome Size and Ploidy Levementioning

confidence: 99%

Flow cytometric determination of genome size and ploidy level of some frost-resistant cultivars and species of Rhododendron L. native to Asian Russia

Ambros¹,

Karakulov²,

Novikova³

2018

Bot Pac

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Somatic polyploidy and its consequences for flower coloration and flower morphology in azalea

Cited by 28 publications

References 33 publications

Sports and hybrids of triploid Hosta ‘Sum and Substance’ reveal chromosome losses and gains in all three apical layers

Sports and hybrids of triploid Hosta ‘Sum and Substance’ reveal chromosome losses and gains in all three apical layers

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

Flow cytometric determination of genome size and ploidy level of some frost-resistant cultivars and species of Rhododendron L. native to Asian Russia

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