Viral infections pose significant threats to plant health, impacting agriculture and medicinal plant production. This study focuses on diagnosing viral infections in <i>Ipomoea cairica</i> (L.) Sweet using advanced techniques such as Transmission Electron Microscopy (TEM) and Microtomy. Phenotypic symptoms, anatomical changes, and the confirmation of Gemini virus through TEM are investigated. Transmission studies reveal multiple vectors, including <i>Bemisia tabaci</i>, highlighting the importance of understanding viral spread mechanisms. Host range studies demonstrate widespread susceptibility among various plant families. The research emphasizes early diagnosis and management to mitigate economic losses and preserve medicinal metabolites. Future efforts will employ PCR and DNA sequencing for precise viral strain identification and targeted management strategies. The findings underscore the importance of early diagnosis and management of viral infections to safeguard plant health and preserve essential metabolites. Future research aims to utilize PCR and DNA sequencing for precise identification and targeted management of viral strains, enabling effective disease control strategies for <i>Ipomoea cairica</i> (L.) Sweet and similar plant species. This study underscores the necessity for proactive measures to protect plant species like <i>Ipomoea cairica</i> (L.) Sweet from viral infections, ensuring sustainable agriculture and medicinal plant production.