Some aspects of induced resistance to rice blast fungus, Magnaporthe grisea, in rice plant infested by white-backed planthopper, Sogatella furcifera

Abstract

The effect of previous rice plant infestation of the white-backed planthopper, Sogatella furcifera, on the incidence of rice blast disease caused by the fungal pathogen, Magnaporthe grisea, was investigated. The fungal pathogen M. grisea was less likely to cause symptoms of leaf blast on rice plants that had been previously infested with S. furcifera than on uninfested plants. This indicates that resistance to M. grisea was induced in rice plants by infestation of S. furcifera. The number of blast lesions per plant decreased with increasing numbers of infesting S. furcifera from 0 to 10 pairs. The active period of induced resistance was maintained for a relatively long time being greater than 15 d. The difference in the incidence of leaf blast between S. furcifera infested and uninfested plants in the booting stage became smaller than that in the tillering stage, although there was a still significant difference in blast incidence between both infested and uninfested plants in booting stage. There was no significant difference in the incidence of leaf blast between damaged plants treated with needling and untreated control plants. This suggests that the simple mechanical effect to the plants caused by stylet insertion (penetration) and subsequent probing with the feeding behavior of S. furcifera was not related to the observed phenomenon. In S. furcifera infested plants, the expression of two genes regarding b-1,3-glucanase, Gns4 and Gns5, was confirmed by RT-PCR analysis. These results show that infestation of S. furcifera apparently induced physiological changes including gene expression that were related to pathogen resistance to M. grisea in rice plants.

Induced systemic resistance against three foliar diseases of Agrostis stolonifera by (2R,3R)‐butanediol or an isoparaffin mixture

“…In O. sativa, infestation by the planthopper, S. furcifera, resulted in ISR against M. grisea and also induction of two β-1,3-glucanases, basic OsGns4 and acidic OsGns5 (Kanno et al, 2005). β-1,3-glucanases can hydrolyze β-1,3-glucans, one of the major components of the cell walls of many fungi (Wessels and Sietsma, 1981).…”

“…In O. sativa, genes encoding two β-1,3-glucanases, Gns4 and Gns5, were found to be induced after treatment with the planthopper, Sogatella furcifera (Kanno et al, 2005). In Triticum aestivum (wheat), a gene encoding a subtilisin-like protein was upregulated after seed treatment with P. aeruginosa (Muyanga et al, 2005).…”

Accumulation of salicylic acid, jasmonic acid and phytoalexins in rice, Oryza sativa, infested by the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)

“…However, the relationships between herbivores and pathogens mediated by the common host plants had hardly ever been studied until recently. Kanno and Fujita (2003) first found that resistance to blast disease caused by the blast fungus, Magnaporthe oryzae B. Couch, in the rice plant, Oryza sativa, is induced by previous infestation of Sogatella furcifera Horváth, which is one of the typical phloem-feeding insect pests, and further confirmed that the induced resistance in the rice is systemic and is caused by the feeding behavior of S. furcifera in laboratory and field conditions (Kanno and Fujita 2003;Kanno et al 2005;Satoh et al 2005). We subsequently H. Kanno Laboratory of Applied Entomology, Kyushu Okinawa National Agricultural Research Center, Kohshi, Kumamoto 861-1192, Japan confirmed the induced resistance to the bacterial leaf blight, Xanthomonas oryzae pv.…”

Isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (Sogatella furcifera)

“…It causes major damage to the rice plants directly by sucking excess plant sap from the phloem and indirectly by transmitting rice plant diseases of economic importance such as Erynia delphacis (Entomophthorales) (Matsui et al, 1998). On the other hand, an effect on the rice also could be induced resistance to rice blast fungus Magnaporthe grisea and Pyricularia grisea, after being infested by S. furcifera (Kanno and Fujita, 2003;Kanno et al, 2005).…”