The results of a new search for positronium decays into invisible final states are reported. Convincing detection of this decay mode would be a strong evidence for new physics beyond the Standard Model (SM): for example the existence of extra-dimensions, of milli-charged particles, of new light gauge bosons or of mirror particles. Mirror matter could be a relevant dark matter candidate.In this paper the setup and the results of a new experiment are presented. In a collected sample of about (6.31 ± 0.28) × 10 6 orthopositronium decays, no evidence for invisible decays in an energy window [0,80] keV was found and an upper limit on the branching ratio of orthopositronium o − P s → invisible could be set:Our results provide a limit on the photon mirror-photon mixing strength ǫ ≤ 1.55 × 10 −7 (90% C.L.) and rule out particles lighter than the electron mass with a fraction Q x ≤ 3.4 × 10 −5 of the electron charge. Furthermore, upper limits on the branching ratios for the decay of parapositronium Br(p − P s → invisible) ≤ 4.3 × 10 −7 (90% C.L.) and the direct annihilation Br(e + e − → invisible) ≤ 2.1 × 10 −8 (90% C.L.) could be set.