Some biocompatibility aspects of conducting polymer polypyrrole evaluated with bone marrow-derived stem cells

Vaitkuviene, Aida; Ratautaitė, Vilma; Mikoliūnaitė, Lina; Kašėta, Vytautas; Ramanauskaitė, Giedrė; Biziulevičienė, Gene; Ramanavičienė, Almira

doi:10.1016/j.colsurfa.2013.06.030

Cited by 57 publications

(47 citation statements)

References 21 publications

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“…CS has excellent biocompatibility and has been approved by the US Food and Drug Administration and the European Medicines Agency for biomedical applications in the human body (Dornish, Kaplan, & Arepalli, ). The results in Figure (b, c) are consistent with previous in vitro and in vivo studies where the biocompatibility of genipin cross‐linked CS and PPY was confirmed (Bavariya et al, ; Chen et al, ; Vaitkuviene et al, ).…”

Section: Resultssupporting

confidence: 90%

Electrical stimulation of adipose‐derived mesenchymal stem cells and endothelial cells co‐cultured in a conductive scaffold for potential orthopaedic applications

Zhang

Neoh

Kang

2017

J Tissue Eng Regen Med

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Electrical stimulation (ES) has emerged as a useful tool to regulate cell behaviour, but the effect of ES on mesenchymal stem cell (MSC)/vasculogenic cell co-culture has not been investigated. Herein, human adipose-derived MSCs (AD-MSCs) and umbilical vein endothelial cells (HUVECs) were co-cultured in an electrically conductive polypyrrole/chitosan scaffold. Compared with AD-MSC monoculture, calcium deposition in the co-culture without and with ES (200 μA for 4 h/day) was 139% and 346% higher, respectively, after 7 days. As the application of ES to AD-MSC monoculture only increased calcium deposition by 56% compared with that without ES after 7 days, these results indicate that ES and co-culture with HUVECs have synergistic effects on AD-MSCs' osteogenic differentiation. ES application also significantly enhanced CD31 expression of HUVECs. In HUVEC monoculture, application of ES increased CD31 expression by 224%, whereas the corresponding increase in AD-MSC/HUVEC co-culture with ES application was 62%. The gene expression results indicate that ES enhanced the cellular functions in AD-MSC and HUVEC monoculture via autocrine bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF), respectively. In co-culture, crosstalk between AD-MSCs and HUVECs due to paracrine BMP-2 and VEGF enhanced the cellular functions compared with the respective monoculture. With application of ES to the AD-MSC/HUVEC co-culture, autocrine signalling was enhanced, resulting in further promotion of cellular functions. These findings illustrate that co-culturing AD-MSC/HUVEC in a conductive scaffold with ES offers potential benefits for bone defect therapy.

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Section: Resultssupporting

confidence: 90%

Electrical stimulation of adipose‐derived mesenchymal stem cells and endothelial cells co‐cultured in a conductive scaffold for potential orthopaedic applications

Zhang

Neoh

Kang

2017

J Tissue Eng Regen Med

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“…The biocompatibility of ICPs has been extensively investigated both in vitro and in vivo. The reported results indeed demonstrated that the ICPs, especially PPy and poly(3,4-ethylenedioxythiophene) (PEDOT), possessed good biocompatibilities with cells, tissues, and organs [18][19][20][21][22][23]. Vaitkuviene et al showed that the PPy nanoparticles synthesized by oxidative polymerization exhibited almost no cytotoxicity to the primary mouse embryonic fibroblasts, mouse hepatoma cell line, and human T lymphocyte Jurkat cell line at low concentrations (up to 9.7 μg mL -1 ) [19].…”

Section: Introductionmentioning

confidence: 89%

“…Vaitkuviene et al showed that the PPy nanoparticles synthesized by oxidative polymerization exhibited almost no cytotoxicity to the primary mouse embryonic fibroblasts, mouse hepatoma cell line, and human T lymphocyte Jurkat cell line at low concentrations (up to 9.7 μg mL -1 ) [19]. They also demonstrated that the PPymodified gold surface showed good biocompatibilities to support the adhesion and proliferation of mouse bone marrow-derived stem cells, similar to the bare gold and polystyrene surfaces [20]. Wang et al reported that the PPy extraction solution showed no trace of acute and subacute toxicity, pyretogen, hemolysis, allergen, and mutagenesis.…”

Section: Introductionmentioning

confidence: 99%

Conducting Polymer-Based Composite Materials for Therapeutic Implantations: From Advanced Drug Delivery System to Minimally Invasive Electronics

Liu

Yin

Chen

et al. 2020

International Journal of Polymer Science

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Conducting polymer-based composites have recently becoming popular in both academic research and industrial practices due to their high conductivity, ease of process, and tunable electrical properties. The multifunctional conducting polymer-based composites demonstrated great application potential for in vivo therapeutics and implantable electronics, including drug delivery, neural interfacing, and minimally invasive electronics. In this review article, the state-of-the-art conducting polymerbased composites in the mentioned biological fields are discussed and summarized. The recent progress on the synthesis, structure, properties, and application of the conducting polymer-based composites is presented, aimed at revealing the structure-property relationship and the corresponding functional applications of the conducting polymer-based composites. Furthermore, key issues and challenges regarding the implantation performance of these composites are highlighted in this paper.An efficient drug delivery system that can deliver the drug to targeted body sites and control the drug release rate precisely is able to improve the therapeutic outcomes and reduce the side effects [36,37]. Structuring such drug delivery systems has been long dreamed of and became more and more practical with the development of a variety of polymer-based delivery systems. From nonbiodegradable diffusion-controlled membranes [38] to biodegradable systems with a combination of diffusion and polymer matrix degradation [39], the polymer-based delivery system has shown enormous benefits in drug delivery and release. And since the 1980s, an effective and intelligent drug delivery system based on the ICPs has been developed [40,41]. Resulting from their inherent electrical, magnetic, and optical properties, the ICPs, especially their composites, are 2

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“…[118,119]. A CNT-CP composite material can impart conductivity and show superior synergistic conductivity compared with CP and CNT, according to the results of a recent research for developing a conductive hydrogel [120].…”

Section: Hybrid Materialsmentioning

confidence: 99%

Incorporation of Conductive Materials into Hydrogels for Tissue Engineering Applications

Min¹,

Koh²

2018

Preprint

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In the field of tissue engineering, conductive hydrogels have been the most effective biomaterials to mimic the biological and electrical properties of tissues in the human body. The main advantages of conductive hydrogel include not only its physical properties, but also its adequate electrical properties, thus providing electrical signals to cells efficiently. However, when introducing a conductive material into a non-conductive hydrogel, a conflicting relationship between the electrical and mechanical properties may develop. This review examines the strengths and weaknesses of the generation of conductive hydrogels using various conductive materials and introduces the use of these conductive hydrogels in tissue engineering applications.

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Some biocompatibility aspects of conducting polymer polypyrrole evaluated with bone marrow-derived stem cells

Cited by 57 publications

References 21 publications

Electrical stimulation of adipose‐derived mesenchymal stem cells and endothelial cells co‐cultured in a conductive scaffold for potential orthopaedic applications

Electrical stimulation of adipose‐derived mesenchymal stem cells and endothelial cells co‐cultured in a conductive scaffold for potential orthopaedic applications

Conducting Polymer-Based Composite Materials for Therapeutic Implantations: From Advanced Drug Delivery System to Minimally Invasive Electronics

Incorporation of Conductive Materials into Hydrogels for Tissue Engineering Applications

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