Some characteristics and short-term preservation of spermatozoa of Deccan mahseer, Tor khudree (Sykes)

Abstract

Over the years, the natural stocks of Tor khudree (Cyprinidae) have depleted due to anthropogenic activities and hence it is considered a threatened species in India. Several in situ and ex situ conservation strategies have been suggested for the revival of T. khudree stocks. The total volume of milt obtained from hormone-injected (gonadotropin-releasing hormone analogue1domperidone) T. khudree were signi¢cantly higher (six to seven times) than that of uninjected ¢sh. The total number of spermatozoa per ¢sh was also higher in injected ¢sh (6.5 Â 10 8^7 .6 Â 10 8 ) than that obtained from uninjected counterparts (1.3 Â 10 8^1 .8 Â 10 8 ). On the contrary, the spermatozoa density and spermatocrit were found to be lower in injected ¢sh than that of the controls. Spermatozoa density and spermatocrit ranged between 4.1 Â 10 8^4 .4 Â 10 8 spermatozoa mL À 1 and 38.1^39.4%, respectively, in injected ¢sh, whereas the ¢gures £uctuated between 6.0 Â 10 8^7 .8 Â 10 8 spermatozoa mL À 1 and 61.5^63.1%, respectively, in uninjected ¢sh. However, there was no signi¢cant di¡erence in the spermatozoa motility rates between experimental and control ¢sh. Di¡erent spermatozoa-activating media revealed no signi¢cant di¡erence in spermatozoa motility between hormoneinjected and uninjected mahseers. But motility duration was the longest with NaCl1urea (190^193 s) and the shortest with tap water (50^55 s) in the experimental and control groups. Short-term preservation of the spermatozoa of T. khudree indicated that spermatozoa stored at 4 1C had higher motility rates than those preserved at room temperature either in the presence or absence of oxygen.

A Study on the Physical and Biochemical Characteristics of Semen of Tor putitora- An Endangered Fish Species in Himalayan Water

“…In cold water snow trout species, it is comparatively less up to 59.7 ±16.5 sec . In Tor khudree, spermatozoa motility lasts for 60sec (Basavaraja and Hegde, 2005). Our finding on sperm motility duration (48.16 ± 1.68 sec) for Tor putitora is very close to the earlier reports on coldwater fish species.…”

Cold storage of the sperm of the endemic trout Oncorhynchus mykiss nelsoni: a strategy for short-term germplasm conservation of endemic species

“…Short-term storage has been considered a useful strategy for preservation of threatened or endangered fish species (Basavaraja and Hegde, 2005;Maria et al, 2006a;Hatipoðlu and Akçay, 2010). Therefore, it may be a worthy strategy for the conservation of the endemic San Pedro Mártir trout (SPMT), Oncorhynchus mykiss nelsoni, which inhabits the streams of the western slopes of Sierra San Pedro Mártir (Ruiz-Campos and Pister, 1995) and is considered the southernmost coastal rainbow trout in North America (Behnke, 2002).…”

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

“…Activation of salmonid species eggs like rainbow trout ( Oncorhynchus mykiss ) could be controlled, and they are more suitable for storage (Goetz & Coffman, ). Although storage approaches of eggs (Ginatullina, Komrakova, & Holtza, ; Goetz & Coffman, ; Holcomb et al, ; Komrakova & Holtz, ) and spermatozoa (Basavaraja & Hegde, ; Kowalski et al, ; Stoss & Holtz, ; Trigo et al, ) have been separately reported, studies on storage of both gametes are limited (Babiak & Dabrowski, ; Jensen & Alderdice, ) even no available data on storage of them used the same medium.…”

“…Successful commercial production of rainbow trout like other fish species is highly related to obtaining and preserving of gametes in good‐quality. Motility percentage, duration of forward motion and curvilinear velocity are the most commonly used parameters for evaluation of spermatozoa quality (Basavaraja & Hegde, ; Billard & Cosson, ). The appearance, size and morphology of eggs could be used to evaluate quality of eggs (Bromage et al, ; Mansour, Lahnsteiner, & Patzer, ).…”