Some Characteristics of the Oral Cavity and Teeth of Cosmonauts  on Missions to the International Space Station

Ilyin, Vyacheslav; Shumilina, G.A.; Solovieva, Z.O.; Nosovsky, A. M.; Kaminskaya, E.V.

doi:10.21687/0233-528x-2016-50-6-25-30

Cited by 3 publications

(2 citation statements)

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“…The immunochemical analysis of gingival fluid in the examined test subjects showed a decrease in the level of immunoglobulins (sIgA, IgA, and IgM). A decrease in the sIgA level may indicate the possibility of an inflammatory process in the periodontal tissues [43].…”

Section: Resultsmentioning

confidence: 99%

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Kononikhin

Brzhozovskiy

Ryabokon

et al. 2019

IJMS

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Comprehensive studies of the effects of prolonged exposure to space conditions and the overload experienced during landing on physiological and biochemical changes in the human body are extremely important in the context of planning long-distance space flights, which can be associated with constant overloads and various risk factors for significant physiological changes. Exhaled breath condensate (EBC) can be considered as a valuable subject for monitoring physiological changes and is more suitable for long-term storage than traditional monitoring subjects such as blood and urine. Herein, the EBC proteome changes due to the effects of spaceflight factors are analyzed. Thirteen EBC samples were collected from five Russian cosmonauts (i) one month before flight (background), (ii) immediately upon landing modules in the field (R0) after 169–199 days spaceflights, and (iii) on the seventh day after landing (R+7). Semi-quantitative label-free EBC proteomic analysis resulted in 164 proteins, the highest number of which was detected in EBC after landing (R0). Pathways enrichment analysis using the GO database reveals a large group of proteins which take part in keratinization processes (CASP14, DSG1, DSP, JUP, and so on). Nine proteins (including KRT2, KRT9, KRT1, KRT10, KRT14, DCD, KRT6C, KRT6A, and KRT5) were detected in all three groups. A two-sample Welch’s t-test identified a significant change in KRT2 and KRT9 levels after landing. Enrichment analysis using the KEGG database revealed the significant participation of detected proteins in pathogenic E. coli infection (ACTG1, TUBA1C, TUBA4A, TUBB, TUBB8, and YWHAZ), which may indicate microbiota changes associated with being in space. This assumption is confirmed by microbial composition analysis. In general, the results suggest that EBC can be used for noninvasive monitoring of health status and respiratory tract pathologies during spaceflights, and that the obtained data are important for the development of medicine for use in extreme situations. Data are available from ProteomeXchange using the identifier PXD014191.

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Section: Resultsmentioning

confidence: 99%

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Kononikhin

Brzhozovskiy

Ryabokon

et al. 2019

IJMS

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“…Другими значимыми причинами нарушения микробиоценоза полости рта могут быть кариесогенные процессы, механическое и химическое отбеливание для улучшения внешнего вида, удаление зубов, хронические заболевания желудочно-кишечного тракта, заболевания верхних дыхательных путей как в анамнезе, так и в текущий период времени, профессио-нальные вредности, наличие у больных профессий, связанных с нервно-эмоциональным напряжением (космонавты, моряки дальнего плавания, подводники, жители Заполярья) и т. д. [3][4][5][6].…”

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Comparison of PCR Diagnostic Method and Mass Spectrometry of Microbial Markers Method as Applied to the Evaluation of Oral Microbiota

Ilyin

Solovieva

Гизингер

et al. 2023

RCLD

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Rapid informative methods for assessing the species and quantitative composition of the microflora of the periodontal and oropharyngeal covering tissues are necessary for operative diagnostics, including those of the dentofacial system. The use of classical bacteriological methods, including seeding, incubation, counting and identification of microorganisms takes up to 5 days, resulting in a significant delay in obtaining the necessary information, which makes it difficult to carry out operative treatment measures. Therefore, the search for means and methods of operative microbiological control is urgent. The present work is devoted to substantiation of MSMM (mass spectrometry of microbial markers) technology application as a means of microbiological control of periodontal and other oropharyngeal biotopes.

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Features of Human Microflora and Local Immunity in an Artificial Environment

Ilyin

Solovyova

Sheblayeva

2022

IJIM

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The article shows the role of extreme inﬂuences on the development of pathological changes in the dentomandibular system. The possibility of developing a system of preventive measures non-biocidal action, mainly using probiotics, in particular autoprobiotics is considered.Introduction. Artiﬁcially modiﬁed habitats are created by humans when they learn the nature of things that lie outside the everyday conditions of existence. This primarily concerns the exploration of space, the ocean and the Earth’s interior. To meet these challenges, artiﬁcial anthropo-ecosystems with modiﬁed habitat parameters are created. Under such altered conditions, the phylogenetically established coactant relationships of the human-micro-organism ecological system undergo changes. It takes the form of a syndrome of impaired colonization resistance [1].The aim of the work was to characterise the three main barriers to infection that form in humans: defence group microﬂora, covering tissues and immunity, in conditions of isolation in hermetically sealed objects with altered environments in long-duration diving, in conditions simulating several factors of space ﬂight (isolation and hypokinesia) and in spaceﬂight conditions.Materials and methods. The determination of immunoglobulins in saliva was carried out using the nephelometric method. The content of immunoglobulins was studied in mixed unstimulated saliva obtained before meals. Monoclonal antibody solutions against sIgA, IgA, IgG antigens were used to determine immunoglobulin concentration. The traditional method of bacteriological analysis was used to identify microorganisms. Bacteriological analysis was performed by seeding on nutrient media (HiCrome Candida Agar, Staphylococcus Agar No10, Columbia Blood Agar Base, Columbia Blood Agar Base + Staph Strepto Supplement, Columbia Blood Agar +Non Spore Anaerobic Supplement).Results and discussion. Insulation in hyperbaric (up to 40 atmospheres) containment facilities.

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Some Characteristics of the Oral Cavity and Teeth of Cosmonauts on Missions to the International Space Station

Cited by 3 publications

References 0 publications

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Comparison of PCR Diagnostic Method and Mass Spectrometry of Microbial Markers Method as Applied to the Evaluation of Oral Microbiota

Features of Human Microflora and Local Immunity in an Artificial Environment

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