Some effects of 2,4-dichlorophenoxyacetic acid on soluble nucleotides &amp; nucleic acid of soybean seedlings

Key, Joe L.; Hanson, James A.

doi:10.1104/pp.36.2.145

Cited by 49 publications

(27 citation statements)

References 29 publications

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“…Results often depend on whether studies on cell expansion were done with intact plants or with excised parts. A net increase in protein content is usually associated with intact cell extension (6,15,17,30), while excised tissues usually show a net decrease (9,18,30,33). A possible exception to the latter phenomenon was reported by Thimann and Loos (32) for excised potato and artichoke tissue.…”

mentioning

confidence: 96%

“…In excised tissues, a net decrease in RNA is usually associated with cell expansion (10,20,33,35). With corn mesocotyl, growth-promoting concentrations of auxin enhanced the rate of RNA breakdown (20,33), but this was not observed in soybean hypocotyl tissue (18,21). In the expanding zone of excised soybean hypocotyl, auxin enhanced C14-nucleotide incorporation into RNA (21), but this did not occur with corn mesocotyl (20).…”

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confidence: 99%

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Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation

Key¹

1964

Plant Physiol.

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185

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confidence: 96%

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confidence: 99%

Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation

Key¹

1964

Plant Physiol.

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185

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“…Also, Atkinson et al (2,3) have postulated negative feedback regulation in ATP conservation and the maintenance of the "energy charge" of the adenylate system. Although the nucleotides of mature leaves or seedlings of oat, wheat, barley, soybean (4,5,8,15,20,29), sugar beet (25), and tomato (30) and for Jerusalem artichoke tubers (33) and Spirodela (6) have been described, it was of interest to determine the sequential changes that take place during the development and senescence of the leaf.…”

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confidence: 99%

Acid-Soluble Nucleotides of Pinto Bean Leaves at Different Stages of Development

Weinstein¹,

McCune²,

Mancini³

et al. 1969

Plant Physiol.

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Abstract. Acid-soluble nucleotides of unifoliate leaves of Pinto bean plants (Phaseolus vulgaris L.) were determined at young, mature, and senescent stages of development. At least 25 components could be distinguished on the basis of inorganic phosphorus determinations and 37 or more fractions on the basis of 82p labeling, with adenosine di-and triphosphates accounting for 60 % of the total moles of nucleotide. The total nucleotide P and inorganic P, on a fresh weight basis, decreased about 44 % between each stage of leaf development, but decrements in the levels of individual nucleotides varied from this over-all pattern.Minor changes in the relative abundance of the individual nucleotides accompanied aging although the percentage of purine-containing nucleotides decreased with age. Total 82p activity per leaf in the nucleotide pool increased about 3-fold between the young and mature leaves and decreased slightly as leaves became senescent. In general, the specific activities of the nucleotides increased with increased age and adenosine-, guanosine-, uridine-, and cytidine triphosphates and adenosine diphosphate accounted for approximately 90 % of the total activity. The changes in the relative sizes and energy status of the nucleotide pools were not so obvious as the changes in other metabolites that have been reported to accompany aging in leaf tissue.

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“…Etiolated cowpea hypocotyls respond similarly to hypocotyls from etiolated intact soybean seedlings treated with 2,4-D, in that both show increases in DNA (9,12), RNA (4,9,11,12), and protein (11,12). Like soybean (12), cowpea RNA increases are greater than those of either DNA or protein.…”

Section: Resultsmentioning

confidence: 91%

Asparate Transcarbamylase Activity in Etiolated Cowpea Hypocotyls Treated with 2,4-Dichlorophenoxyacetic Acid

Johnson¹,

Niblett²,

Shively³

1973

Plant Physiol.

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Treating etiolated cowpea (Vigna unguiculata) seedlings with 2,4-dichlorophenoxyacetic acid resulted in 2.5-, 3.9-, and 6.5-fold increases in DNA, soluble protein, and RNA, respectively, over untreated controls 84 hours after treatment. Aspartate transcarbamylase activity increased within 12 hours after treatment, and by 84 hours it was almost 12-fold greater than that in the untreated controls. During that time, activity in untreated controls dropped 60%. The assay used '4C-aspartate, which was then separated from the "4C-ureidosuccinate product by Dowex 50 (H+ form) column chromatography. Thin layer chromatography of the reaction product indicated that most of the carbamyl-phosphate-dependent radioactivlity cochromatographed with ureidosuccinate. The reaction has a pH optimum near 10.0 and is inhibited by uridine 5'-phosphate and succinate. The data suggest that aspartate transcarbamylase is important in pyrimidine biosynthesis in 2,4-dichlorophenoxyacetic acid-treated seedlings.Aspartate transcarbamylase (carbamoylphosphate: L-aspartate carbamoyltransferase, EC 2.1.3.2) catalyzes the first reaction unique to pyrimidine biosynthesis: aspartic acid + carbamyl phosphate -* carbamyl aspartate (ureidosuccinate) + P,. This enzyme is regulated by feedback inhibition in several microorganisms (22). The pathway of synthesis of pyrimidine nucleotides in higher plants appears similar to that reported for microorganisms (26). Aspartate transcarbamylase from higher plants is a potential site for feedback regulation of the pathway, as it is inhibited strongly by UMP and somewhat by certain other pyrimidine nucleotides (19,20,28,29 cant increase in the activity of this enzyme should occur following 2, 4-D treatment. MATERIALS AND METHODSPlant Material. Etiolated cowpea (Vigna unguiculata L. Walp cv. Early Ramshorn) seedlings were grown in the dark at 29 to 30 C and 85% relative humidity. Seeds with unbroken seedcoats (15) were surface-sterilized 2 to 3 min in 0.5% NaOCI and planted in trays of moist vermiculite.Five days after planting, seedlings were sprayed to run-off with a 2,4-D solution (1 mg/ml) containing 4% ethanol. Controls received a comparable 4% ethanol spray. Hypocotyls were harvested at 12-hr intervals and stored at -18 C until extracted.Tissue Extraction. Frozen tissues were ground with a cold mortar and pestle in cold (3 C) 0.1 M ethanolamine-HCl buffer, pH 10.0. Five or six hypocotyls were ground per treatment, in 2 ml of buffer per g fresh weight. Extracts were centrifuged at 37,000g and 3 C for 15 min. Aliquots of the supernatant fluid were frozen for later determination of RNA, DNA, and soluble protein. Another portion was dialyzed for 16 to 20 hr in cold 0.05 M ethanolamine-HCl buffer, pH 10.0, for use in enzyme assays.Aspartate Transcarbamylase Assay. Four test tubes containing 0.5 ml of dialyzed enzyme and 0.25 ml of 0.05 M ethanolamine-HCl (pH 10.0) buffer were prepared for each treatment and stored at 3 C until 3 to 8 min before assay, when the tubes were shaken in a water bath at 37 C. The ...

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Some effects of 2,4-dichlorophenoxyacetic acid on soluble nucleotides & nucleic acid of soybean seedlings

Cited by 49 publications

References 29 publications

Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation

Ribonucleic Acid and Protein Synthesis as Essential Processes for Cell Elongation

Acid-Soluble Nucleotides of Pinto Bean Leaves at Different Stages of Development

Asparate Transcarbamylase Activity in Etiolated Cowpea Hypocotyls Treated with 2,4-Dichlorophenoxyacetic Acid

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