Some effects of caerulein on the motility of sheep forestomach and gall bladder

Faustini, R.; Beretta, C.; Cheli, R; Gresti, A. De

doi:10.1016/s0031-6989(73)80044-x

Cited by 8 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems likely that equipotent doses of cerulein and CCK-OP remain between 1 : 10 and 1 : 20, depending on the site of action and the parameters examined here and in the previous study (Romań ski, 2004). The doses of CCK peptides used in this study were also similar to those in other classical reports (Bertaccini et al, 1968;Faustini et al, 1973;Bueno and Praddaude, 1979). The highest doses of both hormones used in this study appear to be pharmacological.…”

Section: Discussionsupporting

confidence: 88%

“…These doses were similar to those used in the present study. It has been well established that 1 ng/kg of BW of cerulein is the minimal effective dose (Bertaccini et al, 1968;Faustini et al, 1973), while 100 ng/kg of CCK-OP is also effective in the stimulation of gallbladder motility (Bueno and Praddaude, 1979). The molecular weight of both peptides is not considerably different (octapeptide v. decapeptide); thus cerulein seems to exhibit a greater affinity to CCK receptors.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

View full text Add to dashboard Cite

Cholecystokinin (CCK) can exert multiple actions on intestinal motility but its effect on the small-intestinal 'minute rhythm' (MR) is virtually unknown. Therefore, the electrical activity from the abomasal antrum, duodenal bulb, duodenum, jejunum and ileum was continuously recorded in six sheep before, during and after slow intravenous administration, of three doses each, of cholecystokinin-octapeptide (CCK-OP) and cerulein. In four of these sheep, two additional electrodes and the strain gauge force transducer were also inserted in the duodenum. Chronic experiments were performed in the fasted and non-fasted animals and saline or CCK peptides were injected during phases 1, 2a or 2b of the duodenal migrating myoelectric complex (MMC). The administration of both CCK peptides in various doses evoked an inhibitory effect mostly in the duodenal bulb, except for the lowest dose of cerulein. The effects of 20 times greater doses of CCK-OP than that of cerulein were more pronounced. The introduction of both CCK peptides during phase 1 of the MMC produced no marked or significant response. In non-fasted animals, the effects of both hormonal peptides, given during phase 2b of the MMC, were often stronger than those given during phase 2a, while in fasted animals the effects of CCK peptides, administered in the course of phases 2a and 2b of the MMC, were similar. Both higher doses of CCK peptides increased the number of spike bursts within the given MR pattern in the duodenum and decreased the incidence of MR mostly in the duodenal bulb. The inhibitory effects of both CCK peptides on the bulbar MR exhibited a dose-response character, though the lowest dose often evoked the slight stimulatory response. It is concluded that CCK principally exerts an inhibitory effect upon the MR in the duodenal bulb and modifies the MR in the duodenum by increasing the spike burst number in a given MR pattern. Both these actions of CCK peptides seem to be physiological. There is a positive relationship between the intensity of the refractory period and the demonstrated effect of CCK in the duodenum.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

View full text Add to dashboard Cite

show abstract

“…These doses were similar to those used in the present study. It has been well established that 1 ng/kg of cerulein is the minimal effective dose (Faustini et al 1973) whereas 100 ng/kg of CCK-OP was also effective (Bueno and Praddaude 1979). It seems likely that equipotent doses of cerulein and CCK-OP remain between 1 : 8-10 and 1 : 15 depending on the site of action and parameters examined.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cholecystokinin-Octapeptide and Cerulein on Phasic and Tonic Components in Ovine Duodenum with Special Reference to the 'Minute Rhythm'

Romanski¹

2007

Acta Vet. Brno

View full text Add to dashboard Cite

Cholecystokinin (CCK) can affect phasic contractions and the minute rhythm (MR) in ovine duodenum but its effect on the tonic component remains unclear. Thus, the aim of this study was to assess whether the hormone exerts significant changes on phasic and tonic components of the duodenal motor activity and on phasic and tonic components of the duodenal MR. Mechanical and electrical activities of the duodenum were recorded in four sheep before and after slow intravenous cholecystokinin octapeptide (CCK-OP, doses 20, 200 and 2000 ng/kg b.w.) and cerulein (doses 1, 10 and 100 ng/kg b.w.) administration in the course of phase 2b of the migrating motor complex. During 5-20-minute periods the area under contraction curve for phasic, tonic and total motor activity was measured for the whole curve and separately for the MR-related activity. It was found that both CCK peptides stimulate phasic and tonic components of the duodenal motor activity as well as both these components of the duodenal MR. The effect of CCK peptides on the tonic component was stronger than on the phasic component. These effects were similar in non-fasted and fasted animals. CCK-OP evoked slightly greater effect than cerulein. The effects of these CCK peptides on phasic and tonic components of the MR were similar. It is concluded that CCK-OP and cerulein stimulate both phasic and tonic components of the duodenal motor activity and phasic and tonic components related to the MR in sheep. , small bowel, myoelectrical and motor activity, cholecystokinin Ruminants

show abstract

“…Thus, cerulein is the useful pharmacological tool for experimental and clinical gastrointestinal motility studies (Niederau et al., 1992; Maczka et al., 1993; Scarpignato et al., 1993; Holte and Kehlet, 2002). In sheep, cerulein activity in these regions is also not smaller than that of CCK (Bertaccini et al., 1968; Faustini et al., 1973; Ormas et al., 1984; Ruckebusch and Soldani, 1985).…”

Section: Introductionmentioning

confidence: 98%

Regional differences in the effects of various doses of cerulein upon the small‐intestinal migrating motor complex in fasted and non‐fasted sheep

Romanski¹

2006

Animal Physiology Nutrition

View full text Add to dashboard Cite

There are no precise data concerning the effect of cerulein on the small-intestinal migrating motor complex (MMC) in sheep. Thus, the effort was directed towards the elucidation of the role of cerulein, a cholecystokinin (CCK) amphibian analogue, in the regulation of MMC cycle in conscious fasted and non-fasted sheep in various regions of the small intestine. In six sheep, equipped with bipolar electrodes in the entire small intestine and with one duodenal strain gauge force transducer in four of these animals, the continuous myoelectric and motor activity recordings were performed. During control period, two normal consecutive MMC cycles were recorded in fasted and non-fasted animals and then cerulein was slowly injected at the dose 1 (during 30 s), 10 (during 30 s or 60 s) or 100 ng/kg (during 30 s, 60 s or 120 s) or infused at the dose 0.5 or 1.5 ng/kg/min during 60 min. After cerulein administration at least two consecutive MMC cycles were also recorded. It was found that cerulein in moderate and higher doses injected in both fasted and non-fasted animals, especially during phase 2a or 2b MMC, inhibited phase 3 in the duodenum in at least one-third of the experiments and significantly increased the MMC cycle duration. The application of 100 ng/kg of cerulein during 120 s in the course of phase 2b MMC prolonged cycle duration from 70 +/- 21 to 113 +/- 19 min (p < 0.05) or from 51 +/- 18 to 113 +/- 19 min (p < 0.01) in fasted and non-fasted sheep respectively. The first phase 3 MMC arriving after cerulein administration at the highest dose was usually abnormal in approximately 60% of the experiments. The second phase 3 observed following the hormone injection was abnormal much less frequently and to the lesser extent. Furthermore, both cerulein doses significantly shortened the duration of phase 3 MMC either in fasted (from approximately 5.5-6.5 cpm to 4-5 cpm) or in non-fasted sheep (from approximately 4.5-5.5 to 3-4.5 cpm). The effects of cerulein exhibited the dose-response character. They were more pronounced when cerulein was injected during phase 2b or 2a MMC when compared with phase 1 MMC. The effects of cerulein on the ileal MMC were less evident, although the inhibition of the ileal phase 3 in response to both higher injected doses was observed occasionally. Infusions of cerulein in non-fasted sheep exerted similar effects as cerulein injections. It was stated that cerulein exerts marked regulatory effect upon the small-intestinal MMC in sheep. Although it remains uncertain whether the efficient doses of cerulein used in the present study correspond to physiological or pharmacological doses of CCK it seems likely that most of these doses were physiological. Thus, it is concluded that cerulein participates in the control of MMC cycles in the digestive and interdigestive states in sheep. It is possible that CCK is a physiological regulator of the ovine small-intestinal MMC.

show abstract

Some effects of caerulein on the motility of sheep forestomach and gall bladder

Cited by 8 publications

References 3 publications

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Effect of Cholecystokinin-Octapeptide and Cerulein on Phasic and Tonic Components in Ovine Duodenum with Special Reference to the 'Minute Rhythm'

Regional differences in the effects of various doses of cerulein upon the small‐intestinal migrating motor complex in fasted and non‐fasted sheep

Contact Info

Product

Resources

About