Some features of somatic embryo maturation of algerian fir

Vooková, B.; Kormuťák, Andrej

doi:10.1079/ivp2002330

Cited by 24 publications

(18 citation statements)

References 14 publications

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“…Despite the low induction frequency of SEs (48.5 SE callus -1 ), the medium containing ABA (0.5 µM) and PEG (60 g L -1 ) very efficiently inhibited precocious germination (4%). Studies have described the ability of PEG to increase the osmolarity of the medium and increase the frequency of somatic embryo induction (Castillón and Kamo 2002, Vooková and Kormuták 2002, Steiner et al 2005, Alkhateeb 2006, Calic-Dragosavac et al 2010, Vale et al 2014. The results achieved with PEG may be related to the non-plasmolyzing osmotic effect of this compound: PEG may be able to induce water stress even if no water actually enters the plant cell (Alkhateeb 2006), and lead to an increased protein synthesis (Heringer et al 2013, Vale et al 2014.…”

Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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“…Maturation is a crucial phase in the somatic embryogenesis process, in which the addition of maturation promoters, such as PEG (KeinonenMetta et al 1996, Vooková and Kormuták 2002, Langhansov et al 2004, Maruyama and Hosoi 2007, Krajnakova et al 2009, Calic-Dragosavac et al 2010, abscisic acid (ABA) (Find 1997, Vahdati et al 2008, Calic-Dragosavac et al 2010, Anandam et al 2012, maltose (Li et al 1998) and activated charcoal (Krajnakova et al 2009, Calic-Dragosavac et al 2010, is crucial to promote the maturation of somatic embryos and conversion into plantlets.…”

Section: Induction Of Embryogenic Culturesmentioning

confidence: 99%

“…Several agents that promote somatic embryo maturation have been used, such as polyethylene glycol (PEG), which is a high molecular weight polymer that is not permeable to the cell plasma membrane and, therefore, is not metabolised (Stasolla and Yeung 2003). PEG has been used in plant and cell cultures of several species to simulate the effects of water stress (El-Tayeb and Hassanein 2000) or to stimulate maturation and conversion of somatic embryos into plantlets, similar to those that originate from seeds, as observed in Pinus sylvestris (Keinonen-Metta et al 1996), Abies numidica (Vooková and Kormuták 2002), Panax ginseng (Langhansov et al 2004), Cryptomeria japonica (Maruyama and Hosoi 2007), Abies cephalonica (Krajnakova et al 2009), Aesculus hippocastanum (Calic-Dragosavac et al 2010) and Carica papaya L. (Koehler et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Heringer¹,

Vale²,

Barroso³

et al. 2013

Theor. Exp. Plant Physiol.

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Among commercial fruits, papaya (Carica papaya L.) is notably one of the most common ones around the world. The aim of the current study was to establish a somatic embryogenesis protocol for the hybrid UENF/CALIMAN 01, elucidating the effects of ). The 60 g L -1 PEG treatment group had the highest average for the number, maturation and conversion of somatic embryos that resulted in plantlets at the end of the process. The addition of PEG to MS medium led to increased protein synthesis. These results are unprecedented for C. papaya hybrid UENF/CALIMAN 01 as the current study demonstrates the development of somatic embryogenesis in this high quality hybrid.

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“…However, when considering the potential for the application of the approach to other conifers the demand for plant growth regulators in the cultivation protocol can differ. The regulation of somatic embryogenesis of firs is even more complicated: investigations of somatic embryogenesis in species and hybrids such as A. alba, A. alba x numidica, Abies balsamea, A. concolor, A. cephalonica, A. fraseri, A. lasiocarpa, A. nordmanniana and A. numidica have been made [10][11][12][13][14][15][16][17][18][19][20][21]. Usually cytokinin(s) alone are able to trigger the process of induction of ESM in fir.…”

Section: Introductionmentioning

confidence: 99%

The role of auxins in somatic embryogenesis of Abies alba

et al. 2011

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The somatic embryogenesis of conifers is a process susceptible to exogenous phytohormonal treatments. We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. However, suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases. Early somatic embryos proliferating on a medium supplemented by auxin had an increased probability of maturing successfully. Exogenous auxin treatment during maturation did not compensate for the auxin deficiency during proliferation.

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Some features of somatic embryo maturation of algerian fir

Cited by 24 publications

References 14 publications

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

The role of auxins in somatic embryogenesis of Abies alba

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