Some growth parameters and antimicrobial activity of a bacteriocin-producing strain Pediococcus acidilactici 13

Altuntaş, Evrim Güneş; Coşansu, Serap; Ayhan, Kamuran

doi:10.1016/j.ijfoodmicro.2010.04.024

Cited by 51 publications

(35 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Beberapa bakteriosin terbaru dari produk fermentasi antara lain : bakteriosin yang diisolasi dari produk daging terfermentasi asal Turki (Con & GoÈ kalp, 2000), bakteriosin asal Brazilia (De Martinis & Freitas, 2003), bakteriosin dari produk fermentasi asal Burkina Faso (Omar et al, 2006), bakteriosin yang diisolasi dari produk daging fermentasi tradisional China (Liu et al, 2008), bakteriosin dari sosis kering (Castro et al, 2011), bakteriosin dari produk daging kering fermentasi asal Brasil (Biscola et al, 2013) dan bakteriosin yang diisolasi dari pasta udang asal Thailand (Kaewkloma et al, 2013). Bakteriosin lain juga dihasilkan oleh strain Lactococcus lactis, Pediococcus acidilactici 13, Lactobacillus plantarum A-1, Lactobacillus curvatus MBSa2, Bacillus licheniformis P40, Lactobacillus plantarum 17.2b, dan Lactobacillus pentosus B96 (Cladera-Olivera et al, 2004;Delgado et al, 2007;Alegria et al, 2010;Altuntas et al, 2010;Hata et al, 2010;Hurtado et al, 2011;Biscola et al, 2013;dan Barbosa et al, 2015).…”

Section: Pendahuluanunclassified

Eksplorasi Bakteriosin dari Bakteri Asam Laktat Asal Rusip Bangka dan Kalimantan

Kusmarwati¹,

Arief²,

Haryati³

2014

JPBKP

View full text Add to dashboard Cite

ABSTRAKPenelitian ini dilakukan untuk memproduksi dan mengkarakterisasi bakteriosin yang dihasilkan oleh bakteri asam laktat yang diisolasi dari rusip. Rusip merupakan produk ikan fermentasi tradisional Bangka. Penelitian ini menggunakan sampel rusip yang berasal dari Bangka dan Kalimantan. Sampel diisolasi hingga diperoleh isolat murni yang selanjutnya dilakukan skrining untuk memperoleh isolat yang mampu menghasilkan aktivitas antibakteri tertinggi. Isolat terpilih selanjutnya diidentifikasi dan digunakan untuk memproduksi bakteriosin kasar. Bakteriosin kasar diuji sensitifitasnya terhadap enzim proteolitik dan dikarakterisasi melalui pengujian stabilitas terhadap suhu tinggi, pH, surfaktan, serta stabilitasnya selama penyimpanan. Hasil penelitian menunjukkan bahwa isolat RK4 menghasilkan bakteriosin yang bersifat sensitif terhadap enzim proteolitik seperti proteinase-K dan papain. Bakteriosin tersebut stabil terhadap suhu tinggi dan pH 2-8 serta menghasilkan aktivitas antibakteri yang tertinggi terhadap Staphylococcus aureus. Hasil identifikasi memperlihatkan bahwa isolat RK4 termasuk dalam j enis Pediococcus pentosaceus I. Bakteriosin dari Pediococcus pentosaceus I tersebut aktivitasnya distimulasi oleh EDTA, sodium dodecyl sulphate (SDS) dan lauryl sarcosine. Namun sebaliknya, bioaktivitasnya tidak dipengaruhi oleh Triton X-100, Tween 20, Tween 80 dan urea. Bakteriosin kasar RK4 stabil pada penyimpanan suhu 37 °C selama 4 minggu dan pada suhu dingin selama 2 minggu.

show abstract

Section: Pendahuluanunclassified

Eksplorasi Bakteriosin dari Bakteri Asam Laktat Asal Rusip Bangka dan Kalimantan

Kusmarwati¹,

Arief²,

Haryati³

2014

JPBKP

View full text Add to dashboard Cite

show abstract

“…The optimum temperatures for the production of pediocin by P. pentosaceus ST18 and P. pentosaceus ACCEL were 30 and 37°C, respectively (21,22). The maximum bacteriocin production by P. acidilactici 13 and P. pentosaceous NCDC 273 was reported at 37°C (8,13). Optimum bacteriocin production by L. casei LA-1 was reported at 26 to 37°C (4).…”

Section: Discussionmentioning

confidence: 93%

“…Similar results were also reported for P. pentosaceus NRC AM4 where growth decreased with increase in NaCl concentrations (19). Pediococcus acidilactici 13 grew up to 10% NaCl, but grew optimally in the absence of NaCl (13). The growth of LAB is sometimes better in the presence of low salt concentration, usually 1% to 2% and is inhibited above 3% NaCl, while few LAB are more resistant to NaCl (13).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Culture Conditions for Bacteriocin Production by Soil Isolates Pediococcus pentosaceus LB44 and Weissella confusa LM85

Kaur

Tiwari

2017

Int J Infect

View full text Add to dashboard Cite

Background: Bacteriocins are antimicrobial proteins or peptides secreted in the culture medium during the growth of bacteria. Bacteriocin production is influenced by various physical parameters such as pH, temperature, NaCl concentration, and carbon source. Objectives: The current study aimed at optimizing the culture conditions for higher growth and production of bacteriocins by the soil isolates, Pediococcus pentosaceus LB44, and Weissella confusa LM85. Methods: Growth of LB44 and LM85 isolates was measured spectrophotometrically at OD600 and bacteriocin production was determined in terms of growth inhibition zone using agar well diffusion assay (AWDA) at different pH (3.0 to 10.0), temperatures (30 to 50°C), NaCl concentrations (2% to 10%), and different concentrations (10 to 40 g/L) of carbon sources, glucose, and lactose. Results: Pediococcus pentosaceus LB44 could demonstrate similar growth and activity at pH 5.0 to 8.0; whereas Weissella confusa LM85 could grow and show activity at pH 5.0 to 10.0, but higher growth was recorded at pH 6.0 to 9.0. The growth and activity of P.

show abstract

“…Using this method, one AU was defined as the area of inhibition zone per volume of bacteriocin sample (mm 2 /ml) (Usmiati & Marwati, 2007). Bacteriocin activity (mm 2 /ml) = AU/ml Lz -Ls V after heated at 80 °C for 15 minutes was crude bacteriocin.The bacteriocin activities of each sample were calculated and the results were presented along with the growth curve (Van Reenen et al, 1998in Altuntas et al, 2010.…”

Section: Bacteriocin Activitymentioning

confidence: 99%

OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS

Indriati

Kusmarwati

Hermana

2014

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

Previous study of bacteriocin production on laboratory scale (100 ml) that used MRS broth medium produced unstable activity of bacteriocin. This study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a isolated from rusip, a traditional Bangkanese fermented fish product. The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2 L), followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity and stability of bacteriocin during storage. Results showed that the optimum temperature and pH for the growth of producer cell were 28 °C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0.45% peptone, 1% yeast extract, 2.84% KH 2 PO 4 , 0.2% NaCl and 0.02% MgSO 4 .7H 2 0) in addition of sucrose as carbohydrate source. Based on the growth curve performed on CM medium with KH 2 PO 4 , the L. lactis ssp. lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was 8000 AU/ml at 24 th hour. Bacteriocin was relatively stable when stored at -20 °C for 1 month with a relative activity of 69.4%. Keywords: optimization, cell growth curve, production of bacteriocins, Lactococcus lactis, rusip ABSTRAKStudi terdahulu mengenai produksi bakteriosin skala laboratorium (100 ml) yang menggunakan media MRS cair menghasilkan aktivitas bakteriosin yang tidak stabil. Oleh karena itu penelitian ini bertujuan untuk mengetahui kondisi dan media pertumbuhan optimum untuk produksi bakteriosin. Bakteri yang digunakan dalam penelitian ini adalah bakteri asam laktat (BAL) Lactococcus lactis ssp. lactis CN1.10a yang diisolasi dari rusip, produk fermentasi ikan tradisional Bangka. Terlebih dahulu bakteri dikultivasi untuk selanjutnya digunakan untuk produksi bakteriosin skala intermediet (2 L). Kemudian dilanjutkan dengan optimasi suhu, pH dan media untuk produksi bakteriosin, penentuan kurva pertumbuhan sel, kurva produksi bakteriosin, aktivitas bakteriosin serta stabilitas bakteriosin selama penyimpanan. Hasil penelitian menunjukkan bahwa suhu dan pH optimum untuk pertumbuhan sel produser adalah 28 °C and pH 6. Aktivitas bakteriosin terbaik diproduksi pada media CM (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH 2 PO 4 , 0,2% NaCl and 0,02% MgSO 4 .7H 2 0) dengan sukrosa sebagai sumber karbohidrat. Berdasarkan kurva pertumbuhan pada CM medium dengan KH 2 PO 4 , maka L. lactis ssp. lactis CN1.10a relatif stabil hingga 48 jam. Bakteriosin yang diproduksi oleh sel tersebut adalah sebesar 8000 AU/ml pada jam ke-24. Bakteriosin relatif stabil ketika disimpan pada suhu -20 °C selama 1 bulan dengan aktivitas relatif 69,4%.Kata Kunci: optimasi, kurva pertumbuhan sel, produksi bakteriosin, Lactococcus lactis, rusip

show abstract

Some growth parameters and antimicrobial activity of a bacteriocin-producing strain Pediococcus acidilactici 13

Cited by 51 publications

References 28 publications

Eksplorasi Bakteriosin dari Bakteri Asam Laktat Asal Rusip Bangka dan Kalimantan

Eksplorasi Bakteriosin dari Bakteri Asam Laktat Asal Rusip Bangka dan Kalimantan

Optimization of Culture Conditions for Bacteriocin Production by Soil Isolates Pediococcus pentosaceus LB44 and Weissella confusa LM85

OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS

Contact Info

Product

Resources

About