Some Pharmacological Actions of Diethyldithiocarbamate on Rabbit and Rat Ileum

Effects of α‐methyl‐p‐tyrosine, p‐chlorophenylalanine, l‐β‐(3,4‐dihydroxyphenyl)alanine, 5‐hydroxytryptophan and diethyldithiocarbamate on the analgesic activity of morphine and methylamphetamine in the mouse

“…A similar conclusion was reached by Colville & Chaplin (1964) using the rat inflamed foot as the analgesic testing method. Diminution of the analgesic activity of morphine by propranolol has been reported in the mouse (Heller, Saavedra & Fischer, 1968), but Fennessy & Lee (1970) (Collins & West, 1968) and although its actions in the brain are more complicated, Carlsson, Lindqvist, Fuxe & Hokfelt (1966) demonstrated a general fall in brain noradrenaline and a rise in dopamine in the brain stems and hemispheres of the rat. Increases in reaction time were obtained with mice pretreated with a-methyl-p-tyrosine in the presence of morphine and methylamphetamine.…”

“…Diethyldithiocarbamate reduced the reaction time obtained with morphine and methylamphetamine. This drug, a dopamine ,8-hydroxylase inhibitor, causes a fall in noradrenaline content and a rise in dopamine in peripheral tissues of the rat and rabbit (Collins & West, 1968) and although its actions in the brain are more complicated, Carlsson, Lindqvist, Fuxe & Hokfelt (1966) demonstrated a general fall in brain noradrenaline and a rise in dopamine in the brain stems and hemispheres of the rat. Increases in reaction time were obtained with mice pretreated with a-methyl-p-tyrosine in the presence of morphine and methylamphetamine.…”

The release of 3H‐dopamine from the isolated rabbit ileum

“…6 N-HCl/ethanol mixture (1:1); this was called fraction II. In a series of fifty experiments with non-isotopic dopamine and noradrenaline added to perfusates using these extraction and separation procedures and the methods of estimation described by Collins & West (1968), the mean percentage recoveries (+S.E.) were 47.9+2.1 and 79.8+1.8, respectively.…”