Some properties of chitinase produced by a potent Aspergillus carneus strain

Sherief, A.A.; El-Sawah, M. M. A.; El-Naby, M. A. Abd

doi:10.1007/bf00184691

Cited by 17 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher or lower pH values showed an adverse effect on the enzyme production. These results are in line with other chitinase production by Aspergillus carneus (Sherief et al, 1991). In contrast, the high level of A. terrus chitinase is observed in culture medium with pH 6.5 (Ghanem et al, 2010).…”

Section: Role Of Fungal Biomass On Chitinase Productionsupporting

confidence: 81%

See 1 more Smart Citation

Production, optimization, characterization and antifungal activity of chitinase produced by Aspergillus terrus

Farag¹,

Al-Nusarie²

2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Chitin is one of the most abundant biopolymers widely distributed in the marine and terrestrial environments. Chitinase enzyme has received increased attention due to its wide range of biotechnological applications. Aspergillus terrus was found to be a good chitinase producer among the five fungi isolated from different soil samples from Al-Jouf city, Saudi Arabia. Maximum production of chitinase was obtained when using 2% of Shrimp-shell powder as a sole carbon source in the fermentation medium. The high level of chitinase production was observed in the culture medium with pH 5 at 30°C for five days at shaking conditions. Some properties of the crude chitinase were studied. In the present study, the antifungal activity of crude A. terrus chitinase was investigated against Apergillus niger, Aspergillus oryzae, Pencillum oxysporium, Rhizoctonia solani, Fusarium oxysporium, Rhizopus sp. and Mucor sp. and also estimated. The chitinase was found to inhibit the growth of some phytopathogenic fungi tested. The present work provides a suitable medium composition for enhancement of chitinase production by A. terrus and some properties of crude enzyme. Moreover, the study reflects the potential of A. terrus chitinase for biotechnological application.

show abstract

Section: Role Of Fungal Biomass On Chitinase Productionsupporting

confidence: 81%

“…These results are in good agreement with those reported by other workers (Sandhya et al, 2004, Sowmya et al, 2012. On the other hand, the highest production of chitinase by other fungi was carried out at optimum temperature ranging from 26 to 28°C (Sherief et al, 1991;Brzezinska and Jankiewicz, 2012).…”

Section: Role Of Fungal Biomass On Chitinase Productionmentioning

confidence: 99%

Production, optimization, characterization and antifungal activity of chitinase produced by Aspergillus terrus

Farag¹,

Al-Nusarie²

2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…A maximum chitinase activity of 47.3 U was obtained after 7 days of fermentation under optimal conditions [12]. The optimal media composition for the best producer, Aspergillus carneus was (in kg/m 3 ): chitin, 10; yeast extract, 3; MgSO 4 á 7H 2 O, 1.5; KCl, 0.5.…”

Section: Screening and Selection Of Microorganisms Producing Chitinasementioning

confidence: 95%

Production of microbial chitinases – A revisit

Felse

Panda

2000

Bioprocess Engineering

View full text Add to dashboard Cite

Production of microbial chitinases have received increased attention in recent years due to its potential application in various ®elds. There has been an all around effort to increase the production of chitinases by using genetically engineered organisms and by incorporating modern fermentation techniques. This review presents a brief outline on the production of microbial chitinases which includes information on random screening and selection of chitinolytic organisms and the rationale behind the use of genetically engineered organisms. The problems and perspectives involved in large scale production of chitinases is discussed with special reference to bioreactor studies and new fermentation methods. The present limitations in the understanding of the chitinase fermentation process is also discussed and the scope for future investigation is outlined in this communication.

show abstract

“…Beyond which the activity slowly declined. Initial pH value of the culture medium of pH 5.0 showed the highest chitinolytic activity in 7-day-old shaken culture using Aspergillus carneus strain [13]. Studies over a different periods of incubation of SR 1 , determined the optimum temperature of chitinase activity was at 30°C ( Table 2).…”

Section: Effect Of Ph and Temperaturementioning

confidence: 99%

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Parani¹,

Shetty²,

Saha³

2011

Indian J Microbiol

View full text Add to dashboard Cite

Serratia marcescens, strain SR 1 was isolated from the local soil of a cultivated farm and it was screened as potent strain for chitinase production. Maximum chitinase production (77.3 u Mh -1 100 -1 ) was observed after 96 h of incubation period with pH 5.5 at 30°C under shake conditions (120 rpm). Compare to still flasks, shake culture with prawn fish colloidal chitin of 0.5% (w/v) concentration, showed a better enzyme yield. Crude enzyme showed antifungal activity against plant pathogens.

show abstract

Some properties of chitinase produced by a potent Aspergillus carneus strain

Cited by 17 publications

References 11 publications

Production, optimization, characterization and antifungal activity of chitinase produced by Aspergillus terrus

Production, optimization, characterization and antifungal activity of chitinase produced by Aspergillus terrus

Production of microbial chitinases – A revisit

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Contact Info

Product

Resources

About