Some properties of pea lipoxygenase isoenzymes

Lipoxygenase heterogeneity in Pisum sativum

“…The results of Northern hybridizations indicate that RNA corresponding to C2 does not hybridize to cDNA clones representing the major seed lipoxygenases; this indicates limited homology between C2 and the clones pPEI036 and pPE923 and may represent a situation analogous to two classes of pea legumin gene which share limited homology at the nucleic acid level Gatehouse et al 1988). It is unlikely that C2 corresponds to lipoxygenase-1 which is a very minor component of pea lipoxygenase (Yoon and Klein 1979), as extensive homology exists between lipoxygenases -1, -2 and -3 from soyabean (Shibata et al 1987(Shibata et al , 1988Yenofsky et al 1988); C2 may correspond to a second minor component identified in pea lipoxygenase by u and Klein (1979).…”

“…1975;Spaapen etal. 1977); later work described two major enzymes of Mr 95000 in pea seeds corresponding to soyabean lipoxygenases-2 and -3, and two minor enzyme fractions, one of which corresponds to soyabean lipoxygenase-1 (Yoon and Klein 1979). All of these analyses are based on the isolation of peaks of enzyme activity from column-chromatographic separations; none has employed Laemmli (1970) sodium dodecyl sulfate (SDS) gel electrophoresis or immunological analyses.…”

Inhibition of Soybean Lipoxygenases – Structural and Activity Models for the Lipoxygenase Isoenzymes Family

“…The activity of soybean lipoxygenase-1 was inhibited by -carotene which breaks the chain reaction at the beginning stage of linoleic acid hydroperoxidation (Serpen & Gökmen, 2006). Besides soybean lipoxygenase (Ikedioby & Snyder, 1977;Hildebrand & Hymowitz, 1982) carotene oxidation during lipoxygenase-mediated linoleic acid oxidation has been reported in various studies for the enzymes extracted from potato (Aziz et al, 1999), pea (Yoon & Klein, 1979;Gökmen et al, 2002), wheat (Pastore et al, 2000), olive (Jaren-Galan et al, 1999) and pepper (JarenGalan & Minguez-Mosquera, 1997). Soybean lipoxygenase-1 and recombinant pea lipoxygenase-2 and lipoxygenase-3, oxidizing -carotene, yield apocarotenal, epoxycarotenal, apocarotenone and epoxycarotenone .…”

Isolation of a pea (Pisum sativum) seed lipoxygenase promoter by inverse polymerase chain reaction and characterization of its expression in transgenic tobacco

“…Pea seeds have two major LOX polypeptides [5,44] which have amino acid sequences, predicted from cDNA clones, that correspond to those of LOX-2 and LOX-3 from soybean seeds [13,14]. The pea LOX polypeptides, and their corresponding RNAs, appear at a mid-late stage of pea seed development [12] and the RNA amounts increase with seed fresh weight.…”