Some properties of the DNA from a new equine herpesvirus

Antigenic and Biochemical Analysis of gB of Herpes Simplex Virus Type 1 and Type 2 and of Cross-reacting Glycoproteins Induced by Bovine Mammillitis Virus and Equine Herpesvirus Type 1

“…Several studies have demonstrated relationships between HSV-1, HSV-2 and BMV, both genetically with the BMV genome showing 14~ base sequence homology (Sterz et al, 1973/4) and the HSV-2 genome showing 47~ base sequence homology with HSV-1 (Kieff et al, 1972;Sugino & Kingsbury, 1976) and antigenically by complement fixation, immunodiffusion, immunofluorescence, immunoprecipitation and neutralization (Sterz et al, 1973/4;Killington et al, 1977Killington et al, , 1978Norrild et al, 1978 ;Yeo et al, 1981). However, HSV-1 shows less than 5 ~ base sequence homology with EHV-1 (Ludwig et al, 1971) and although antigenic cross-reactions between HSV-I and EHV-1 have been demonstrated by complement fixation, gel diffusion, immunofluorescence and immunoprecipitation (Plummet, 1964;Blue & Plummer, 1973;Killington et al, 1977;Yeo et al, 1981) the viruses do not cross-neutralize. More recently, Davison & Wilkie (1983) examined the regions of the HSV-1 and HSV-2 genomes to which EHV-1, PRV and varicella-zoster virus DNA would hybridize and concluded that these herpesviruses possessed several highly conserved genes.…”

Immunologic and Genetic Relationship between Herpes Simplex Virus and Bovine Herpes Mammillitis Virus

“…Bovine herpes mammillitis infection of test animals protected them against a fatal herpes simplex virus infection. The DNA of the bovine herpes mammillitis virus (63% guanadine + cytosine; G + C) shared about 14% base sequence homology with that of herpes simplex virus type 1 (66% G + C), whereas the genetic relatedness with the infectious bovine rhinotracheitis and pscudorabies virus was less than 8%.Besides the closely related herpes simplex virus types 1 and 2 (HSV-1, HSV-2) of man [21,27,28,31,32], which exhibit a high degree of genetic relationship [13,18,35], several other herpesviruses share common antigens [9,11,14,25,37], but lack DNA-DNA or DNA-RNA homology of more than about 10% [3,5,17,19,38], The bovine herpes mammillitis (BHM) virus, an established member of the herpes group [20], which is responsible for vari ous skin infections in bovine animals, has been found during the course of our serologic and epidemiologic studies to share common immunologic properties with the herpes simplex viruses of man. The purpose of the work reported here was to investigate the antigenic relationship between human and bovine herpesviruses and to correlate this with genetic homology by DNA-DNA hybridization.…”

Replication of Equine Herpesvirus Type 1 and Type 3: Resistance to Hydroxyurea and Thymidine

“…3). One species (p = 1.700 g/cm3) corresponded in buoyant density to equine cell DNA [13], and the second species (p = 1.716 g/cm3) corresponded to EHV-1 DNA [16,23]. The presence of the inhibitors HU or TdR had little effect on the amount of EHV-1 DNA synthesis.…”

“…Only one major species of DNA, having a buoyant density equivalent to that reported for EHV-3 DNA (p = 1.725 g/cm3) [13], was synthesized in EHV-3-infected cells after 4 h p. i.; this finding is consistent with the rapid and total inhibition of host DNA synthesis reported to occur after infection with EHV-3 [22]. As noted in EHV-1-infected cultures, cells labeled early after infection with EHV-3 exhibited some rescue from TdR inhibition of host DNA synthesis ( fig.4C,D).…”

“…The genital strain of equine herpesvirus, equine herpesvirus type 3 (EHV-3), has been shown to differ significantly from EHV-1 in a number of biological, biochemical, and biophysical characteristics [12][13][14], Therefore, the capacity of EHV-3-infected cells to synthesize viral DNA and infectious virus in the presence of HU was investigated. Moreover, the ability of both equine herpes viruses to replicate in the presence of excess thymidine was also examined.…”