Some Studies on Ornithobacterium Rhinotracheale (Ort) in Broiler Chickens

Abstract

Affected 100 flocks revealed chronic whitish diarrhea. Consistent gross pathological lesions recorded were congestion and consolidation of lungs, hemorrhages in trachea, congestion and hemorrhages in liver, splenomegaly and necrotic foci on the kidneys, mortality in various flocks ranged from 3.7 -15% detailed isolation and biochemical studies revealed that Ornithobacterium Rhinotracheale was responsible for this wide spread respiratory problem. Five ORT isolate positive PCR implications were pre elicited to be the size of 784 bpfinally it was confirmed that sequence analysis of 16 sr RNA and ORT isolates from Gen bank with identified from 94% to 98%. Antibacterial sensitivity revealed that most of the isolates were sensitive to lincospectin (lincomycin + spectinomycin) and doxycycline. Many of the isolates showed resistance to kanamycin, norfloxacin, enrofloxacin, tetracycline and chloramphenicol. Experimental infection of broiler orally with ORT evoked respiratory signs with mortality reached 20% lesions of moderated saculitis mild tracheitis and unilateral pneumonia were recorded during the observation period. On other hand of broiler infected with ORT revealed that the infected and treated broiler were lower significance than infected and treated with lincospectin. The microscopical examination revealed pathological changes can be seen in lung, trachea, liver, spleen and brain.

Molecular Detection of The Ornithobacterium rhinotracheale From Turkeys Flocks in Alexandria Governorate During 2020 – 2021

“…Ellakany et al (2019) revealed ORT isolates were detected in Broilers and layers with a percent of 11.66%and closely related to Asian, European, and American strains (98%-100%). Masoud et al (2015) showed a similarity percent of 94%-98% among the selected five broiler's ORT strains and some Asian, and American ones. Thieme et al (2016) when compared the results of 16S rRNA gene sequencing and multilocus sequence typing (MLST) in 65 isolates of ORT, they demonstrated identity ranging 85.1%-100%.…”

“…In Egypt, Youssef and Ahmed (1996); El-Gohary (1998), El-Gohary and Awaad (1998); El-Gohary et al (1998); El-Gohary and Sultan (1999); Abd El-Avian disease Ghany (2000) isolated ORT from broiler, turkey, and layer flocks either alone or concomitantly with other organism as well as other many studies were done on isolation, characterization, and treatment of ORT (Amal, 2002;Shihata and Ibraheem, 2004;Shahata et al, 2006;Attia, 2008;Elbestawy, 2010;Hegazy et al, 2015;Masoud et al, 2015;El-Abasy et al, 2016;Ellakany et al, 2019;Hassan et al, 2020). Vandamme et al (1994) described ORT bacterium as highly polymorphic Gram-negative rod, non-motile, non-spore former in family Flavobacteriaceae.…”