Some Unusual Properties of Pakistan ‘Shoti’ Starch

Ithaca, Thomas J. Schoch; Maywald, Eileen C.

doi:10.1002/star.19680201103

Cited by 10 publications

(1 citation statement)

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“…Increases of the corresponding peak areas were proportional to the amount of exogenous standards, thus corroborating the peak identities and the absence of co-eluted compounds. The method was further validated by analyzing already characterized antisense potato starches with altered phosphorus contents (Blennow et al, 2005;Kozlov, Blennow, Krivandin & Yuryev, 2007;Viksø-Nielsen, Blennow, Jørgensen, Kristensen, Jensen & Møller, 2001) as well as the highly phosphorylated shoti starch (Ithaca & Maywald, 1968)(Figure 1b). Consistent with previous descriptions, Glc-3-P and Glc-6-P peak areas were increased in both shoti starch and the antisense potato line asBE, whereas they were significantly decreased in starch from the asGWD line (Figure 1b).…”

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confidence: 99%

Rapid and sensitive quantification of C3- and C6-phosphoesters in starch by fluorescence-assisted capillary electrophoresis

Verbeke

Penverne

d’Hulst

et al. 2016

Carbohydrate Polymers

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Phosphate groups are naturally present in starch at C3-or C6-position of the glucose residues and impact the structure of starch granules. Their precise quantification is necessary for understanding starch physicochemical properties and metabolism. Nevertheless, reliable quantification of Glc-3-P remains laborious and time consuming. Here we describe a capillary electrophoresis method for simultaneous measurement of both Glc-6-P and Glc-3-P after acid hydrolysis of starch. The sensitivity threshold was estimated at the fg scale, which is compatible with the analysis of less than a g of sample. The method was validated by analyzing antisense potato lines deficient in SBEs, GWD or GBSS. We show that Glc-3-P content is altered in the latter and that these variations do not correlate with modifications in Glc-6-P content. We anticipate the method reported here to be an efficient tool for high throughput study of starch phosphorylation at both C3-and C6position.

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