Sophisticated polycaprolactone/gelatin nanofibrous nerve guided conduit containing platelet-rich plasma and citicoline for peripheral nerve regeneration: In vitro and in vivo study

Samadian, Hadi; Ehterami, Arian; Sarrafzadeh, Arash; Khastar, Hossein; Nikbakht, Mohammad Reza; Rezaei, Aram; Chegini, Leila; Salehi, Majid

doi:10.1016/j.ijbiomac.2020.02.102

Cited by 77 publications

(50 citation statements)

References 67 publications

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“…A commercial electrospinning device (Fanavaran-nano-meghyas Ltd co Tehran Iran) was used to fabricate GNFs based on our previous study 39 . Briefly, based on our previous study 40 , a 40% (w/v) concentration gelatin solution was obtained by dissolving gelatin powder in AA aqueous [75% (v/v)] under stirrer at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Samadian

Farzamfar

Vaez

et al. 2020

Sci Rep

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The focus of the current study was to develop a functional and bioactive scaffold through the combination of 3D polylactic acid (PLA)/polycaprolactone (PCL) with gelatin nanofibers (GNFs) and Taurine (Tau) for bone defect regeneration. GNFs were fabricated via electrospinning dispersed in PLA/PCL polymer solution, Tau with different concentrations was added, and the polymer solution converted into a 3D and porous scaffold via the thermally-induced phase separation technique. The characterization results showed that the scaffolds have interconnected pores with the porosity of up to 90%. Moreover, Tau increased the wettability and weight loss rate, while compromised the compressive strengths. The scaffolds were hemo- and cytocompatible and supported cell viability and proliferation. The in vivo studies showed that the defects treated with scaffolds filled with new bone. The computed tomography (CT) imaging and histopathological observation revealed that the PLA/PCL/Gel/Tau 10% provided the highest new bone formation, angiogenesis, and woven bone among the treatment groups. Our finding illustrated that the fabricated scaffold was able to regenerate bone within the defect and can be considered as the effective scaffold for bone tissue engineering application.

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Section: Methodsmentioning

confidence: 99%

“…The liquid displacement method was used to determine the porosity of the prepared scaffolds based on Eq. ( 2 ) 39 where V 1 is the initial volume of absolute ethanol, V 2 is its volume after scaffold soaking (and ethanol filled the pores), and V 3 is the volume of the ethanol after the scaffold removal.…”

Section: Methodsmentioning

confidence: 99%

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Samadian

Farzamfar

Vaez

et al. 2020

Sci Rep

Self Cite

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“…Nanotechnology is enabling approaches dealing with subjects at the size scale lower than 100 nm [25, 26]. Nanotechnology and nanomaterials are involving in various fields of medical sciences and technology such as drug delivery [27–29], tissue engineering [30–32], wound dressing [33–35], cancer detection and therapy [36, 37]. Dendrimers are a versatile class of polymeric nanostructures known as highly branched, globular, nanosized, highly monodispersed, multifunctional, and highly symmetrical polymeric three‐dimensional (3D) structures [38–40].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, radiolabelling, and biological assessment of folic acid‐conjugated G‐3 ^99m Tc‐dendrimer as the breast cancer molecular imaging agent

Zamani

Shafeie-Ardestani

Bitarafan-Rajabi

et al. 2020

IET nanobiotechnol.

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“…The obtained results can be attributed to the berberine-induced reduction in the physical interaction between the polymer chains. Berberine weakened interchains and intrachain physical interaction and forces such as van der Waals forces and hydrogen bonding 47,48 . However, the obtained tensile strengths are in the acceptable range.…”

mentioning

confidence: 99%

Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

Samadian

Zamiri

Ehterami

et al. 2020

Sci Rep

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207

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Functional wound dressing with tailored physicochemical and biological properties is vital for diabetic foot ulcer (DFU) treatment. Our main objective in the current study was to fabricate Cellulose Acetate/ Gelatin (CA/Gel) electrospun mat loaded with berberine (Beri) as the DFU-specific wound dressing. The wound healing efficacy of the fabricated dressings was evaluated in streptozotocin-induced diabetic rats. The results demonstrated an average nanofiber diameter of 502 ± 150 nm, and the tensile strength, contact angle, porosity, water vapor permeability and water uptake ratio of CA/Gel nanofibers were around 2.83 ± 0.08 MPa, 58.07 ± 2.35°, 78.17 ± 1.04%, 11.23 ± 1.05 mg/cm 2 /hr, and 12.78 ± 0.32%, respectively, while these values for CA/Gel/Beri nanofibers were 2.69 ± 0.05 MPa, 56.93 ± 1°, 76.17 ± 0.76%, 10.17 ± 0.21 mg/cm 2 /hr, and 14.37 ± 0.42%, respectively. The antibacterial evaluations demonstrated that the dressings exhibited potent antibacterial activity. The collagen density of 88.8 ± 6.7% and the angiogenesis score of 19.8 ± 3.8 obtained in the animal studies indicate a proper wound healing. These findings implied that the incorporation of berberine did not compromise the physical properties of dressing, while improving the biological activities. In conclusion, our results indicated that the prepared mat is a proper wound dressing for DFU management and treatment. Diabetes mellitus is classified as a metabolic disease that has various complications such as chronic wounds, arterial damage, and neuropathy resulting from uncontrolled blood sugar. The wound healing process is a complex and multiphase process that is delayed in diabetic patients because of various complexities 1,2. In these patients, the angiogenesis and re-epithelialization are inadequate because of low interaction between growth factors and their target site. Severe inflammation is an additional deleterious factor resulting from neutrophil infiltration. Moreover, diabetic foot ulcer (DFU) is another complication that is the consequence of intense inflammation,

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Sophisticated polycaprolactone/gelatin nanofibrous nerve guided conduit containing platelet-rich plasma and citicoline for peripheral nerve regeneration: In vitro and in vivo study

Cited by 77 publications

References 67 publications

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Synthesis, radiolabelling, and biological assessment of folic acid‐conjugated G‐3 ^99m Tc‐dendrimer as the breast cancer molecular imaging agent

Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

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Sophisticated polycaprolactone/gelatin nanofibrous nerve guided conduit containing platelet-rich plasma and citicoline for peripheral nerve regeneration: In vitro and in vivo study

Cited by 77 publications

References 67 publications

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Synthesis, radiolabelling, and biological assessment of folic acid‐conjugated G‐3 99m Tc‐dendrimer as the breast cancer molecular imaging agent

Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

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Product

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Synthesis, radiolabelling, and biological assessment of folic acid‐conjugated G‐3 ^99m Tc‐dendrimer as the breast cancer molecular imaging agent