Sophora subprostrate polysaccharide regulates histone acetylation to inhibit inflammation in PCV2-infected murine splenic lymphocytes in vitro and in vivo

Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic Lymphocytes

“…To elucidate the underlying molecular mechanism of the intervention of SSP1 in PCV2-induced inflammatory responses, the transcripts were identified between these three groups (C, V, SV group). The sequencing results showed that PCV2 infection can induce up-regulated expression of chemokines and inflammatory factors such as CXCL9, CXCL10, CXCL11, TNF-α, and IL-1β, while SSP1 can downregulate the expression levels of these factors, which is consistent with previous reports [12]. In the present study, pro-inflammatory signaling molecules (IRF, STAT, SOCS) and pro-inflammatory cytokinerelated genes (TNF-α, CXCL9, CXCL10) occupy a more important position in differential expression enrichment analysis.…”

“…Much evidence showed that Sophora subprostrate polysaccharides have potential applications in the prevention of chronic infectious diseases. In our previous study, PCV2-infected murine splenic lymphocytes could induce an inflammatory response, while SSP1 could modulate this inflammatory damage [12]. Therefore, to understand the underlying ncRNA mechanism of SSP1 in regulating PCV2-induced splenic lymphocyte injury in mice, the transcripts were annotated in samples using RNA-seq to analyze differentially expressed mRNA, LncRNA, miRNA and circRNA and their complex regulatory relationships, verify the reliability of differentially expressed genes by RT-qPCR and the expression of key signaling pathway proteins for DE mRNAs enrichment by western blot, which provided a basis for us to understand the regulatory mechanism of SSP1.…”

“…In our previous research, both the PCV2 SH strain and PCV2NJ2002 were used to explore the inflammation-causing effect in murine spleen lymphocytes. The levels of inflammatory response in PCV2-infected murine spleen lymphocytes were significantly increased, and SSP1 could alleviate PCV2-induced inflammatory injury [12,27]. PCV2 SH strain [12] and PCV2NJ2002 [27] showed similar effects during the experiment, and SSP1 treatment at 400 µg/mL showed good anti-inflammatory effects.…”

“…The levels of inflammatory response in PCV2-infected murine spleen lymphocytes were significantly increased, and SSP1 could alleviate PCV2-induced inflammatory injury [12,27]. PCV2 SH strain [12] and PCV2NJ2002 [27] showed similar effects during the experiment, and SSP1 treatment at 400 µg/mL showed good anti-inflammatory effects. To investigate the effect of SSP1 on PCV2-infected cells on gene regulation, the following three groups were set up: Control (C), PCV2 infection group (PCV2, V), and SSP1 treatment group (PCV2 + 400 µg/mL SSP1, SV).…”

“…Sophora subprostrate polysaccharide is one of the main bioactive components of Chinese herbal Sophora subprostrate, with a variety of biological activities such as anti-inflammation, antioxidant and immunomodulation [9][10][11]. Previous studies have found that Sophora subprostrate polysaccharides can inhibit the inflammatory response of splenic lymphocytes induced by PCV2 infection by regulating the expression of histone acetylation-related genes and proteins [12], but the mechanisms by which Sophora subprostrate polysaccharides regulate the inflammatory response of murine splenic lymphocytes is still unclear, especially the specific way the Sophora subprostrate polysaccharide regulates the inflammatory signaling pathways still needs to be explored.…”