Sorting of Lytic Enzymes in the PlantGolgi Apparatus

Abstract

The sections in this article areIntroductionGeneral Features of the Lytic Vacuole PathwayEvidence for Receptor‐Mediated Sorting: The Roles ofVSRProteins in Plant CellsBP‐80 as a Sorting Receptor: Proof of Function in YeastStructural Requirements for Ligand Binding byVSRProteinsChimeric Integral Membrane Reporter Proteins as Probes of theGolgi to Lytic Vacuole PathwayThe Lytic Prevacuolar Compartment: Identity and FunctionAcknowledgements

Biogenesis of Plant Prevacuolar Multivesicular Bodies

“…This compartment is characterized by the presence of acid hydrolases to degrade cellular materials that are no longer required. Intracellular transport of these hydrolytic enzymes from their site of synthesis, the ER, occurs via the Golgi apparatus, where they are sorted from secretory proteins (Raymond et al, 1992;Barr, 2002;Jiang and Rogers, 2003). On the basis of precedents from mammalian and yeast cells, prelysosomal compartments (PLCs) or the PVCs are intermediate organelles between the TGN and the lytic compartment, for the transport of materials away from the secretory pathway (Lemmon and Traub, 2000;Luzio et al, 2003).…”

“…At the late Golgi or TGN, soluble proteins without vacuolar sorting signals will be secreted outside of the cell by default. Soluble vacuolar proteins with sorting signals are recognized by receptor proteins in the TGN and further transported into PVCs/MVBs, where receptors are recycled back to the TGN for another round of cargo binding, while cargo proteins are finally deposited into lytic vacuole (LV) (Hadlington and Denecke, 2000;Jiang and Rogers, 2003;Tse et al, 2009;Shen et al, 2013a). This paradigm for post-Golgi pro-tein trafficking has often been used as a guideline for plant scientists in discussions on vacuolar protein transport (Foresti and Denecke, 2008;Robinson et al, 2008;Rojo and Denecke, 2008;Gao et al, 2012;Wang et al, 2014;Lin et al, 2015;Woo et al, 2015).…”

Protein trafficking in plant cells: Tools and markers

“…Contrary to mammalian and yeast cells, plant cells contain two types of vacuoles that have distinct morphology and functions: lytic vacuoles (LVs) and protein storage vacuoles (PSVs) . The plant LV is functionally equivalent to the animal lysosome and the yeast in terms of protein degradation and has an acid pH, while the PSV is unique to plant cells for protein storage with a neutral pH environment (Jiang et al, 2001;Jiang and Rogers, 2003). The conventional protein transport pathways in the plant endomembrane system are summarized in Figure 1.…”