SOS-red fluorescent protein (RFP) bioassay system for monitoring of antigenotoxic activity in plant extracts

Bartolome, Amelita; Mandap, Katheryn; David, Kevim; Sevilla, Fortunato; Villanueva, James

doi:10.1016/j.bios.2005.10.009

Cited by 20 publications

(15 citation statements)

References 23 publications

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“…However, other chemical investigations are needed to identify the compounds which could not be detected by GC-MS. Grover & Bala (1993) reported that, a pretreatment with an aqueous guava leaf extract was found to be effective in activating the mutagenicity of direct acting mutagens. Moreover, it has been reported that the aqueous whole plant extract afforded protection against mitomycin C, nalidixic acid and hydrogen peroxide induced mutagenicity (Bartolome et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antimutagenic potential of Psidium guajava leaf extracts

Zahin

Ahmad

Aqil

2016

Drug and Chemical Toxicology

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Fruits, vegetables and medicinal herbs rich in phenolics antioxidants contribute toward reduced risk of age-related diseases and cancer. In this study, Psidium guajava leaf extract was fractionated in various organic solvents viz. petroleum ether, benzene, ethyl acetate, ethanl and methanol and tested for their antioxidant and antimutagenic properties. Methanolic fraction showed maximum antioxidant activity comparable to ascorbic acid and butylated hydroxyl toluene (BHT) as tested by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe3 + reducing power) and CUPRAC (cupric ions (Cu) reducing ability) assays. The fraction was analyzed for antimutagenic activities against sodium azide (NaN), methylmethane sulfonate (MMS), 2-aminofluorene (2AF) and benzo(a)pyrene (BP) in Ames Salmonella tester strains. The methanol extracted fraction at 80 μg/ml concentration inhibited above 70% mutagenicity. Further, phytochemical analysis of methanol fraction that was found to be most active revealed the presence of nine major compounds by gas chromatography-mass spectrometry (GC-MS). This data suggests that guava contains high amount of phenolics responsible for broad-spectrum antimutagenic and antioxidant properties in vitro and could be potential candidates to be explored as modern phytomedicine.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and antimutagenic potential of Psidium guajava leaf extracts

Zahin

Ahmad

Aqil

2016

Drug and Chemical Toxicology

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“…A considerable battery of assays exists for the detection of different genotoxic effects of compounds in experimental systems, or for investigations of exposure to genotoxic agents in environmental or occupational settings. Treatment with the aqueous whole plant extracts of Psidium guajava afforded protection (anti-genotoxic activity) against mitomycin C, nalidixic acid and hydrogen peroxide (three genotoxins) (Bartolome et al, 2006). In another study, a pre-treatment with an aqueous guava leaf extract was found to be effective in inactivating the mutagenicity of direct-acting mutagens 4-nitro-o-phenylenediamine and 2-aminofluorene in the tester strains of Salmonella typhimurium.…”

Section: Antigenotoxic and Antimutagenic Effectsmentioning

confidence: 97%

Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology

Gutierrez¹,

Mitchell

Solís

2008

Journal of Ethnopharmacology

747

449

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“…Performance was usually poorer compared with bioluminescent recA ‐based reporters: lag times were longer and detection thresholds were higher, unless incubation times were very long. The recA ′:: DsRed plasmid, hosted in E. coli UTL2, was used in order to monitor antigenotoxic activity of plant extracts that exhibited some protection against MMC, NA and hydrogen peroxide (Bartolome et al. , 2006).…”

Section: Reporter Systemsmentioning

confidence: 99%

Bacterial genotoxicity bioreporters

Biran

Yagur‐Kroll

Pedahzur

et al. 2010

Microbial Biotechnology

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SummaryEver since the introduction of the Salmonella typhimurium mammalian microsome mutagenicity assay (the ‘Ames test’) over three decades ago, there has been a constant development of additional genotoxicity assays based upon the use of genetically engineered microorganisms. Such assays rely either on reversion principles similar to those of the Ames test, or on promoter–reporter fusions that generate a quantifiable dose‐dependent signal in the presence of potential DNA damaging compounds and the induction of repair mechanisms; the latter group is the subject of the present review. Some of these assays were only briefly described in the scientific literature, whereas others have been developed all the way to commercial products. Out of these, only one, the umu‐test, has been fully validated and ISO‐ and OECD standardized. Here we review the main directions undertaken in the construction and testing of bacterial‐based genotoxicity bioassays, including the attempts to incorporate at least a partial metabolic activation capacity into the molecular design. We list the genetic modifications introduced into the tester strains, compare the performance of the different assays, and briefly describe the first attempts to incorporate such bacterial reporters into actual genotoxicity testing devices.

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SOS-red fluorescent protein (RFP) bioassay system for monitoring of antigenotoxic activity in plant extracts

Cited by 20 publications

References 23 publications

Antioxidant and antimutagenic potential of Psidium guajava leaf extracts

Antioxidant and antimutagenic potential of Psidium guajava leaf extracts

Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology

Bacterial genotoxicity bioreporters

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