Sound wave activated nano-sieve (SWANS) for enrichment of nanoparticles

Habibi, Ruhollah; Neild, Adrian

doi:10.1039/c9lc00369j

Cited by 42 publications

(49 citation statements)

References 98 publications

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“…Samples such as cells and microorganisms can be manipulated without harmful damage from electric or magnetic fields, and without cross-contamination due to direct contact between manipulator and sample [ 3 ]. An acoustofluidic miniaturized system composed of piezoelectric substrate and closed microchannel/chamber has been widely used in particle sorting [ 4 ], washing [ 5 ], enrichment [ 6 ], and arrangement [ 7 ]. The acoustic radiation force (ARF) generated by surface acoustic waves can accurately manipulate and capture particles or cells suspended in liquid, which has great application potential [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Detection in Droplet Microfluidics by Acoustic Vortex Modulation of Particle Rings and Particle Clusters via Asymmetric Propagation of Surface Acoustic Waves

Liu

et al. 2022

Biosensors

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As a basis for biometric and chemical analysis, issues of how to dilute or concentrate substances such as particles or cells to specific concentrations have long been of interest to researchers. In this study, travelling surface acoustic wave (TSAW)-based devices with three frequencies (99.1, 48.8, 20.4 MHz) have been used to capture the suspended Polystyrene (PS) microspheres of various sizes (5, 20, 40 μm) in sessile droplets, which are controlled by acoustic field-induced fluid vortex (acoustic vortex) and aggregate into clusters or rings with particles. These phenomena can be explained by the interaction of three forces, which are drag force caused by ASF, ARF caused by Leaky-SAW and varying centrifugal force. Eventually, a novel approach of free transition between the particle ring and cluster was approached via modulating the acoustic amplitude of TSAW. By this method, multilayer particles agglomerate with 20 μm wrapped around 40 μm and 20 μm wrapped around 5 μm can be obtained, which provides the possibility to dilute or concentrate the particles to a specific concentration.

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Section: Introductionmentioning

confidence: 99%

Enhanced Detection in Droplet Microfluidics by Acoustic Vortex Modulation of Particle Rings and Particle Clusters via Asymmetric Propagation of Surface Acoustic Waves

Liu

et al. 2022

Biosensors

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“…Acoustic field-based separation and concentration of EVs has been proposed in a number of realizations (Wu et al, 2017 ; Habibi and Neild, 2019 ). Conventionally, acoustic isolation technologies subject the fluid to differential acoustic forces and particles are laterally displaced in proportion with their size.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Conventionally, acoustic isolation technologies subject the fluid to differential acoustic forces and particles are laterally displaced in proportion with their size. Recently, a sound wave activated nano-sieve was estimated to have 50-fold nanoparticle enrichment capability (Habibi and Neild, 2019 ). The advantages of acoustic isolation include straightforward manufacturing, and contactless particle separation (Wu et al, 2017 ).…”

Section: Experimental Approachesmentioning

confidence: 99%

Characterizing Extracellular Vesicles and Their Diverse RNA Contents

2020

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Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular communication agents that regulate physiological states by transporting biomolecules between near and distant cells. The research community has consistently advocated for the importance of RNA contents in EVs by demonstrating that: (1) EV-related RNA contents can be detected in a liquid biopsy, (2) disease states significantly alter EV-related RNA contents, and (3) sensitive and specific liquid biopsies can be implemented in precision medicine settings by measuring EV-derived RNA contents. Furthermore, EVs have medical potential beyond diagnostics. Both natural and engineered EVs are being investigated for therapeutic applications such as regenerative medicine and as drug delivery agents. This review focuses specifically on EV characterization, analysis of their RNA content, and their functional implications. The NIH extracellular RNA communication (ERC) program has catapulted human EV research from an RNA profiling standpoint by standardizing the pipeline for working with EV transcriptomics data, and creating a centralized database for the scientific community. There are currently thousands of RNA-sequencing profiles hosted on the Extracellular RNA Atlas alone (Murillo et al., 2019 ), encompassing a variety of human biofluid types and health conditions. While a number of significant discoveries have been made through these studies individually, integrative analyses of these data have thus far been limited. A primary focus of the ERC program over the next five years is to bring higher resolution tools to the EV research community so that investigators can isolate and analyze EV sub-populations, and ultimately single EVs sourced from discrete cell types, tissues, and complex biofluids. Higher resolution techniques will be essential for evaluating the roles of circulating EVs at a level which impacts clinical decision making. We expect that advances in microfluidic technologies will drive near-term innovation and discoveries about the diverse RNA contents of EVs. Long-term translation of EV-based RNA profiling into a mainstay medical diagnostic tool will depend upon identifying robust patterns of circulating genetic material that correlate with a change in health status.

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“…As with many other research approaches in acoustic manipulation, early work started with separating particles and did not focus on biological objects [ 37 , 86 ]. Habibi and colleagues, for example [ 87 ], trapped microparticles in acoustic nodes of a microchannel and managed to thereby trap nanoparticles that were attracted by the microbeads. Acoustofluidics were also used to trap cells to perform single-cell analysis [ 88 ].…”

Section: Acoustic Patterning On Different Timescalesmentioning

confidence: 99%

The waves that make the pattern: a review on acoustic manipulation in biomedical research

Guex

Marzio

Eglin

et al. 2021

Materials Today Bio

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Novel approaches, combining technology, biomaterial design, and cutting-edge cell culture, have been increasingly considered to advance the field of tissue engineering and regenerative medicine. Within this context, acoustic manipulation to remotely control spatial cellular organization within a carrier matrix has arisen as a particularly promising method during the last decade. Acoustic or sound-induced manipulation takes advantage of hydrodynamic forces exerted on systems of particles within a liquid medium by standing waves. Inorganic or organic particles, cells, or organoids assemble within the nodes of the standing wave, creating distinct patterns in response to the applied frequency and amplitude. Acoustic manipulation has advanced from micro- or nanoparticle arrangement in 2D to the assembly of multiple cell types or organoids into highly complex in vitro tissues. In this review, we discuss the past research achievements in the field of acoustic manipulation with particular emphasis on biomedical application. We survey microfluidic, open chamber, and high throughput devices for their applicability to arrange non-living and living units in buffer or hydrogels. We also investigate the challenges arising from different methods, and their prospects to gain a deeper understanding of in vitro tissue formation and application in the field of biomedical engineering.

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Sound wave activated nano-sieve (SWANS) for enrichment of nanoparticles

Abstract: Ultrasonic actuation of a packed bed of microbeads enables the entrapment and enrichment of highly-diluted nanoparticles. The approach offers the possibility of future upscaling and high throughput.

Cited by 42 publications

References 98 publications

Enhanced Detection in Droplet Microfluidics by Acoustic Vortex Modulation of Particle Rings and Particle Clusters via Asymmetric Propagation of Surface Acoustic Waves

Enhanced Detection in Droplet Microfluidics by Acoustic Vortex Modulation of Particle Rings and Particle Clusters via Asymmetric Propagation of Surface Acoustic Waves

Characterizing Extracellular Vesicles and Their Diverse RNA Contents

The waves that make the pattern: a review on acoustic manipulation in biomedical research

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