Sources of resistance against the Pepper yellow mosaic virus in chili pepper

Abstract

The Pepper yellow mosaic virus (PepYMV) naturally infects chili and sweet pepper, as well as tomato plants in Brazil, leading to severe losses. This work reports the reaction to the PepYMV of 127 Capsicum spp. accessions, aiming at identifying resistance sources useful in breeding programs. The experiment was carried out in a completely randomized design, with eight replications, in greenhouse conditions. Plants were protected with an insect-proof screen to avoid virus dissemination by aphids. Leaves of Nicotiana debneyi infected with the PepYMV were used as the inoculum source. Plants were inoculated with three to four fully expanded leaves. A second inoculation was done 48 hours later to avoid escapes. Only the youngest fully expanded leaf was inoculated. Two plants were inoculated only with buffer, as negative control. Symptoms were visually scored using a rating scale ranging from 1 (assymptomatic plants) to 5 (severe mosaic and leaf area reduction). Nine accessions were found to be resistant based on visual evaluation. Their resistance was confirmed by ELISA. Two resistance accessions belong to the species C. baccatum var. pendulum, while the seven other were C. chinense. No resistant accessions were identified in C. annuum var. annuum, C. annuum var. glabriusculum, and C. frutescens.

Determining the inheritance of agronomic traits in chili pepper

“…Its fruits are elongated with length around of 10 cm and diameter of 2.7 cm. The parent UENF 1732 was also collected in a local market in Campos dos Goytacazes, and it is characterized as resistant to PepYMV (Bento et al, 2009), showing fruits of orange color in the immature stage and red at maturity, with campanulate shape, mean length of 4,4 cm and diameter of 4,5 cm.…”

Gene effect and heterosis in Capsicum baccatum var. pendulum

“…pendulum produced by GONÇALVES et al (2011), usi ng a genetic full diallel design without reciprocals, were evaluated. The following accessions were used as parents: UENF 1616, UENF 1624, UENF 1629, UENF 1639 and UENF 1732 from the Germplasm Collection of the Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), characterized by MOURA et al (2010) and evaluated for resistance to pepper yellow mosaic virus by BENTO et al (2009) (Table 1).…”

Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre- and post-harvest

“…from the germplasm collection of the Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) were assessed. These accessions were previously characterized by Sudré et al (2005) and Bento et al (2007) according to morphological and agronomic descriptors; and by Bento et al (2009), for resistance to Pepper yellow mosaic virus (PepYMV; genus Potyvirus, family Potyviridae). The accessions were sown in trays of 128 cells with organic-vegetable substrate.…”

“…Among these resistant accessions of C. baccatum var. pendulum, accessions UENF 1624, UENF 1732, UENF 1764 and UENF 1770 were also considered resistant to PepYMV by Bento et al (2009). Regarding the ripe fruit, only six accessions did not develop symptoms, four of which were C. chinense accessions (UENF 1554, UENF 1703, UENF 1706 and UENF 1751).…”