SOX21-AS1 is associated with clinical stage and regulates cell proliferation in nephroblastoma

Zhang, Jingxiu; Hou, Tianzhao; Qi, Xueliang; Wang, Jihong; Sun, Xiangguo

doi:10.1042/bsr20190602

Cited by 24 publications

(19 citation statements)

References 21 publications

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“…For example, SOX21-AS1 participates in cervical cancer progression by competitively binding miR-7/voltage dependent anion channel 1 ( 57 ), while it sequesters miR-145 in CRC, which promotes tumor progression via the enhanced expression of myosin VI ( 60 ). SOX21-AS1 knockdown in nephroblastoma cells in vitro disrupts the proliferation and colony formation of these cells through a mechanism associated with p57 upregulation, and results in cell cycle arrest ( 62 ). In addition, SOX21-AS1 is associated with HCC progression and patient prognosis via a mechanism associated with p21 epigenetic silencing ( 58 ).…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Jiang

Zhang

et al. 2021

Oncol Lett

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Gastric cancer (GC) is a common type of cancer, and identification of novel diagnostic biomarkers associated with this disease is important. The present study aimed to identify novel diagnostic biomarkers associated with the prognosis of GC, using an integrated bioinformatics approach. Differentially expressed long non-coding RNAs (lncRNAs) associated with GC were identified using Gene Expression Omnibus datasets (GSE58828, GSE72305 and GSE99416) and The Cancer Genome Atlas database. A competing endogenous RNA network that incorporated five lncRNAs [long intergenic non-protein coding RNA 501 (LINC00501), LINC00365, SOX21 antisense divergent transcript 1 (SOX21-AS1), GK intronic transcript 1 (GK-IT1) and DLEU7 antisense RNA 1 (DLEU7-AS1)], 29 microRNAs and 114 mRNAs was constructed. Gene Ontology and protein-protein interaction network analyses revealed that these lncRNAs may be involved in ‘biological regulation’, ‘metabolic process’, ‘cell communication’, ‘developmental process’, ‘cell proliferation’, ‘reproduction’ and the ‘cell cycle’. The results of receiver operating characteristic curve analysis demonstrated that LINC00501 (AUC=0.819), LINC00365 (AUC=0.580), SOX21-AS1 (AUC=0.736), GK-IT1 (AUC=0.823) and DLEU7-AS1 (AUC=0.932) had the potential to become valuable diagnostic biomarkers for GC. Associations with clinicopathological characteristics demonstrated that LINC00501 expression was significantly associated with sex (P=0.015) and tumor grade (P=0.022). Furthermore, LINC00365 expression was significantly associated with lymph node metastasis (P=0.025). Gene set enrichment analysis revealed that LINC00501, LINC00365 and SOX21-AS1 were enriched in signaling pathways associated with GC. Reverse transcription-quantitative PCR analysis demonstrated that LINC00501 expression (P=0.043) was significantly upregulated in GC tissues, whereas the expression levels of LINC00365 (P=0.033) and SOX21-AS1 (P=0.037) were significantly downregulated in GC tissues. Taken together, the results of the present study suggest that LINC00501, LINC00365, SOX21-AS1, GK-IT1 and DLEU7-AS1 may be used as novel diagnostic biomarkers for GC, and may be functionally associated with GC development and progression.

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Section: Discussionmentioning

confidence: 99%

Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Jiang

Zhang

et al. 2021

Oncol Lett

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“…The previous study has been shown that the nonmuscle myosin light chain kinase isoform, cloned from MYLK gene, was found highly expressed in an endothelial cell in lung cancer (Adyshev et al, 2013). Another study has also shown that lncRNA SOX21 antisense RNA 1 was elevated in tissue and cell lines of nephroblastoma in comparison with adjacent normal tissues and cell line of the healthy human embryonic kidney (Zhang et al, 2019). In addition, MYLK‐AS1 is also elevated in hepatocellular carcinoma and also engaged in higher risk of death (Ye et al, 2019).…”

Section: Discussionmentioning

confidence: 98%

Silencing of long noncoding RNA MYLK‐AS1 suppresses nephroblastoma via down‐regulation of CCNE1 through transcription factor TCF7L2

Zhu

Gao

Tang

et al. 2021

Journal Cellular Physiology

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Nephroblastoma, a pediatric kidney cancer, caused by pluripotent embryonic renal precursors. Long noncoding RNAs (lncRNAs) are commonly abnormal expressed in many cancers. In the present study, we fousced on one newly discrovered lncRNA, MYLK Antisense RNA 1 (MYLK‐AS1), and its functional role in proliferation and cycle distribution of nephroblastoma cells. Micorarray‐based analysis revealed the highly expressed Cyclin E1 (CCNE1) and MYLK‐AS1 in nephroblastoma. After nephroblastoma tissue sample collection, RT‐qPCR confirmed the upregulated expression of MYLK‐AS1 and CCNE1 in nephroblastoma tissues and cells. Kaplan–Meier curve exhibited that patients with elevated CCNE1 had lower overall survival rate in follow‐up study. RNA binding protein immunoprecipitation, chromatin immunoprecipitation, and dual‐luciferase reporter gene assay were employed to determine the relationship among MYLK‐AS1, TCF7L2, and CCNE1, which validated that transcription factor 7‐like 2 (TCF7L2) could specifically bind to MYLK‐AS1 and TCF7L2 could positively promote CCNE1. After gain‐ and loss‐of function assays, the conclusion that silencing of MYLK‐AS1 could inhibit expression of CCNE1 through the transcription factor TCF7L2 to regulate the cell proliferation and cell cycle distribution of nephroblastoma cells was obtained. Subsequently, the subcutaneous tumor formation ability of nephroblastoma cell in nude mice was observed and the silencing of MYLK‐AS1 exerts suppressive role in the tumorigenic ability of nephroblastoma cells in vivo. Taken together, MYLK‐AS1 constitutes a promising biomarker for the early detection and treatment of nephroblastoma.

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“…63 SOX21-AS1 has been linked to tumor progression and defined as a prognostic marker in cervical cancer, nephroblastoma, hepatocellular carcinoma, and lung and oral cancer. [64][65][66][67][68][69] In glioblastoma, SOX21-AS1 was defined along other four lncRNAs as a signature that predicts survival. 70 CRNDE is an important oncogenic lnRNA implicated in multiple malignancies including glioblastoma.…”

Section: Discussionmentioning

confidence: 99%

Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles

et al. 2020

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Therapy resistance and recurrence in high-grade gliomas are driven by their populations of glioma stem cells (GSCs). Thus, detailed molecular characterization of GSCs is needed to develop more effective therapies. We conducted a study to identify differences in the splicing profile and expression of long non-coding RNAs in proneural and mesenchymal GSC cell lines. Genes related to cell cycle, DNA repair, cilium assembly, and splicing showed the most differences between GSC subgroups. We also identified genes distinctly associated with survival among patients of mesenchymal or proneural subgroups. We determined that multiple long noncoding RNAs with increased expression in mesenchymal GSCs are associated with poor survival of glioblastoma patients. In summary, our study established critical differences between proneural and mesenchymal GSCs in splicing profiles and expression of long non-coding RNA. These splicing isoforms and lncRNA signatures may contribute to the uniqueness of GSC subgroups, thus contributing to cancer phenotypes and explaining differences in therapeutic responses.

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SOX21-AS1 is associated with clinical stage and regulates cell proliferation in nephroblastoma

Cited by 24 publications

References 21 publications

Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Integrative analysis of the gastric cancer long non‑coding RNA‑associated competing endogenous RNA network

Silencing of long noncoding RNA MYLK‐AS1 suppresses nephroblastoma via down‐regulation of CCNE1 through transcription factor TCF7L2

Proneural and mesenchymal glioma stem cells display major differences in splicing and lncRNA profiles

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