Soy isoflavonoids and cancer — metabolism at the target site

Boersma, Brenda J.; Barnes, Stephen; Kirk, M; Wang, C.-C; Smith, Michelle; Kim, H; Xu, Jun; Patel, Rakesh P.; Darley‐Usmar, Victor

doi:10.1016/s0027-5107(01)00175-0

Cited by 45 publications

(23 citation statements)

References 33 publications

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“…The consumption of soy is important in the Asian diet and is associated with a lower risk of prostate cancer in Asian men. Soybean products are rich sources of isoflavones, and their intake in Asian countries exceeds 40 mg/ day, which is at least 10 times greater than that in American countries (Boersma et al, 2001). Thus, the increased consumption of phytoestrogens has contributed significantly to reducing the incidence of cancer (Klein and King, 2007).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the mutagenicity and antimutagenicity of soy phytoestrogens using micronucleus and comet assays of the peripheral blood of mice

Niwa¹,

Oliveira²,

Mantovani³

2013

Genet. Mol. Res.

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ABSTRACT. Studies show that soy imparts many favorable properties in the human body, including the prevention of chronic diseases such as osteoporosis, heart disease, cancer, and diabetes. Soy is rich in isoflavones, and it is a candidate for the chemoprevention of diseases owing to its low toxicity. In this study, a soy phytoestrogen (with high levels of the isoflavones genistin and daidzein) was tested in mice to investigate its mutagenicity and genotoxicity using micronucleus and comet assays of mouse peripheral blood. Phytoestrogen (0.083, 0.83 and 8.3 mg/kg body weight) was evaluated with and without the chemotherapeutic agent cyclophosphamide. For the micronucleus assay, blood was collected before treatment and after 24 and 48 h. For the comet assay, blood was collected only after 24 h. Phytoestrogen was not mutagenic and reduced cyclophosphamide-induced DNA damage. The results from the comet assay revealed a reduction of DNA damage; however, phytoestrogen did induce genotoxic damage during the 24-h treatment. This genotoxic damage could have been repaired and was therefore not identified in the micronucleus assay, which detects mutations. The results suggested that the reduction of DNA damage observed in associated treatments could also reduce the side effects of chemotherapy. Moreover, they suggested that phytoestrogen might be a candidate of interest for the chemoprevention of cancer because it protects against DNA damage.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the mutagenicity and antimutagenicity of soy phytoestrogens using micronucleus and comet assays of the peripheral blood of mice

Niwa¹,

Oliveira²,

Mantovani³

2013

Genet. Mol. Res.

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“…27 Regulation of DNA topoisomerase activity, synthesis and release of Tumor Growth Factor Beta and induction of apoptosis of cancerous cells in vivo are other proposed mechanisms of activity. 28,29 Cell cycle regulation of P21 is also known to be up-regulated by genistein and may play a significant role in determining the potential sensitivity of breast epithelial cells, normal or malignant to genistein. 30,31 Genistein has also been shown to inhibit topoisomerase 2 by inducing the upregulation of P53 protein, thus primarily providing stabilization of the DNA cleavage complex.…”

Section: Biological Mechanisms Of Actionmentioning

confidence: 99%

Soy Isoflavones and Breast Cancer

Wu¹,

Lee²,

Vigen³

2013

American Society of Clinical Oncology Educational Book

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“…Soybean products are rich sources of isoflavones, and the daily ingestion of isoflavones in Asian countries can surpass 40 mg/day, at least 10 times more than on the American continents (BOERSMA et al, 2001). Epidemiologic studies show that the ingestion of foods rich in isoflavones confers protection against various types of cancer, particularly those that are hormone dependent, such as mammary and prostate cancers (ADLEUCREUTZ et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Modulação do efeito mutagênico do benzo[a]pireno e bleomicina por extratos de isoflavonas em células de hepatoma de roedor

Josiane

Tsuboy

Marcarini

et al. 2012

Semin. Cienc. Biol. Saude

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Modulação do efeito mutagênico do benzo[a]pireno e bleomicina por extratos de isoflavonas em células de hepatoma de roedor Modulation of the mutagenic effect of benzo[a]pyrene and bleomycin by isoflavone extracts in a rat hepatoma cell lineEpidemiologic studies show that the intake of foods rich in isoflavones (phytoestrogens), such as soybeans, confers protection against various types of cancer, what increases the scientific and popular interest on these compounds. In the present study, phytoestrogens extracts from soybeans were tested for genotoxic potential and modulatory effects on benzo[a]pyrene and bleomycin. Two phytoestrogens were evaluated in vitro, phytoestrogen "A" was supplied by EMBRAPA-Soja, Londrina -PR, and phytoestrogen "B" was purchased in a local drug store. The methods used were the comet assay (genotoxicity and antigenotoxicity) and micronucleus test with cytokinesis block (mutagenicity) in rat hepatoma cells (HTC cell). The isoflavones were tested at three concentrations pre-established by the MTT cytotoxicity assay. Both isoflavone extracts showed no genotoxic effects in the comet assay, but showed induction of micronucleus. In the evaluation of the phytoestrogens for a modulatory effect, both phytoestrogens extracts showed antigenotoxicity in the comet assay.

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Soy isoflavonoids and cancer — metabolism at the target site

Cited by 45 publications

References 33 publications

Evaluation of the mutagenicity and antimutagenicity of soy phytoestrogens using micronucleus and comet assays of the peripheral blood of mice

Evaluation of the mutagenicity and antimutagenicity of soy phytoestrogens using micronucleus and comet assays of the peripheral blood of mice

Soy Isoflavones and Breast Cancer

Modulação do efeito mutagênico do benzo[a]pireno e bleomicina por extratos de isoflavonas em células de hepatoma de roedor

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