Soybean-Derived Phytoestrogens Regulate Prostaglandin Secretion in Endometrium During Cattle Estrous Cycle and Early Pregnancy

Wocławek-Potocka, Izabela; Bah, Mamadou M.; Korzekwa, Anna; Piskuła, Mariusz K.; Wiczkowski, Wiesław; Depta, A.; Skarzynski, Dariusz J.

doi:10.1177/153537020523000305

Cited by 76 publications

(99 citation statements)

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“…The concentrations of P4, PGFM and PGE2 in the plasma and medium samples were determined with direct enzyme immunoassays (EIAs), as described previously [28]. The anti-P4 serum was donated by Prof S Okrasa, University of Warmia and Mazury in Olsztyn.…”

Section: Hormone Determinationmentioning

confidence: 99%

Lysophosphatidic Acid Action During Early Pregnancy in the Cow: In Vivo and In Vitro Studies

Wocławek-Potocka

Kowalczyk-Zięba

Skarzynski

2010

Journal of Reproduction and Development

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Abstract. We have previously documented synthesis of lysophosphatidic acid (LPA) in the bovine endometrium and the increased presence of LPA receptor mRNA expression during pregnancy. Therefore, LPA could contribute to early pregnancy establishment in the cow. In the present study, we investigated the effect of intravaginally administered LPA on pregnancy rates and on the plasma levels of progesterone (P4) and prostaglandins (PGs) in heifers. Animals were inseminated and from day 15 to 18 after estrus were treated intravaginally with saline, LPA (1 mg) or LPA receptor blocker (VPC32183; 1 mg). Blood samples were collected on days 0,6,12,15,16,17,18 and 21 after insemination. Pregnancy was confirmed by ultrasonography and per rectum examination on days 30 and 49-50 after insemination. Intravaginal LPA administration increased the plasma P4 and PGE2 concentrations compared with saline and VPC32183-treated heifers. In the saline and LPA-treated groups, 6 out of 8 heifers were pregnant (75%), whereas the pregnancy rate in the VPC32183-treated heifers was only 37%. We also examined the effects of LPA on PG secretion and PG synthase mRNA expression in stromal and epithelial cells of the bovine endometrium on days 16-18 of pregnancy and the estrous cycle. LPA increased PGE2 production and PGE2 synthase (PGES) mRNA expression in stromal cells during the estrous cycle and pregnancy. On Days 16-18 of pregnancy, LPA inhibited PGF2α production and PGFS mRNA expression in epithelial cells. The results suggest that LPA serves as a luteotropic factor during the estrous cycle and pregnancy, stimulating P4 secretion in vivo and PGE2 secretion in vitro through activation of PGES mRNA expression in stromal cells. Moreover, during the early pregnancy, LPA decreases PGF2α synthesis and mRNA expression for PGFS in epithelial cells of the bovine endometrium. Key words: Cow, Lysophosphatidic acid, Pregnancy, Progesterone, Prostaglandins (J. Reprod. Dev. 56: [411][412][413][414][415][416][417][418][419][420] 2010) he role of lysophosphatidic acid (LPA) in the reproductive system has been studied extensively in mice [1], pigs [2,3], ewes [4] and cows [5][6][7]. We found that in the bovine endometrium, in contrast to the mouse, pig and sheep [1][2][3][4], there is mRNA expression only for the LPA receptor type 1 (LPAR1) [5]. We also demonstrated that LPA is locally produced and released from the bovine endometrium [5]. The LPA concentration and expression of mRNA for LPAR1 in the bovine endometrium are significantly higher during early pregnancy than during the estrous cycle. Moreover, LPA administered into the aorta abdominalis stimulated progesterone (P4) and prostaglandin (PG) E2 secretion during the luteal phase of the estrous cycle in vivo. This LPA-mediated stimulation of PGE2 synthesis might serve as an additional luteotropic factor during the luteal phase of the estrous cycle and on days 8-10 of early pregnancy. However, during early pregnancy, on days 8-10 after conception in the cow, LPA may play an even more important role b...

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Section: Hormone Determinationmentioning

confidence: 99%

Lysophosphatidic Acid Action During Early Pregnancy in the Cow: In Vivo and In Vitro Studies

Wocławek-Potocka

Kowalczyk-Zięba

Skarzynski

2010

Journal of Reproduction and Development

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“…Before and during the mating period the C and P diets included 20% of grass meal and 20% of alfalfa meal, respectively. The levels of phytoestrogens in the diets were determined by HPLC (Woclawek-Potocka et al, 2005). Con-centrations of total phytoestrogens in the C and P diets were 210.38 and 383.55 µg/g during the growth period and 15.…”

Section: Animals and Dietsmentioning

confidence: 99%

Effects of dietary meals containing different levels of phytoestrogens on reproductive function in Bilgoraj ganders

Opałka

Kugla-Owczarska

Kamińska

et al. 2008

Acta Veterinaria Hungarica

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The aim of this study was to investigate how long-term feeding of ganders with diets containing different levels of phytoestrogens affects the morphometry of testes, plasma steroid levels, sperm parameters and reproductive performance. Male Bilgoraj geese were fed diets containing grass meal (with low phytoestrogen levels) and those containing alfalfa meal and soybean meal (with higher levels of phytoestrogens). Testes were obtained from ganders at the peak of mating and the ongoing laying period as well as at the beginning of photorefractoriness. The morphometric parameters were measured in frozen testicular sections. Blood was collected monthly from December to June, and plasma testosterone, androstenedione and corticosterone concentrations were determined. During the mating season, semen was collected and its quantity and quality were investigated. In the flocks (ganders with geese) reproductive output was noted. Seasonal changes were observed in the morphology of testes and in plasma testosterone, androstenedione and corticosterone concentrations in ganders. The feeding of ganders with dietary meals containing higher levels of phytoestrogens did not affect the plasma steroid contents or the majority of the morphometric parameters of testes, except for the height of the seminiferous epithelium, which was lowered. Moreover, diets with higher levels of phytoestrogens decreased the volume of ejaculates and the percentage of normal spermatozoa. However, these changes were not reflected in the fertilisation rates.

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“…Moreover, Lundh et al [11] showed in cows that the daidzein and genistein present in fodder are immediately converted in the rumen to equol and para-ethyl-phenol, respectively. We showed in our previous study that there are significant levels of daidzein, genistein and their metabolites in the soy beans fed to dairy cattle [6]. High concentrations of isoflavone metabolites (equol and para-ethyl-phenol) are present in the blood of cows fed soy bean [6,9].…”

mentioning

confidence: 93%

Concentrations of Isoflavones and Their Metabolites in the Blood of Pregnant and Non-pregnant Heifers Fed Soy Bean

et al. 2008

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Abstract. The present study compared the changes in isoflavones (daidzein and genistein) and their metabolite (equol and para-ethyl-phenol) concentrations in the blood plasma of cyclic and pregnant heifers after feeding with soy bean. Twelve healthy heifers were divided into three groups: cyclic heifers (days 8-12 of the estrous cycle; control group; n= 4), an early pregnancy group (2 months pregnant; n=4) and a late pregnancy group (8 months pregnant; n=4). All heifers were fed a single dose of 2.5 kg of soy bean and then blood samples were taken from the jugular vein for 8 h at predetermined intervals. The concentrations of soy bean-derived isoflavones and their active metabolites were measured in the blood plasma on an HPLC system. In the blood plasma of the early-and late-pregnant heifers, we found lower concentrations and time-dependent decreases in daidzein and genistein in comparison to cyclic heifers (P<0.05). Moreover, we noticed significant increases of equol and para-ethyl-phenol in the blood plasma of the earlypregnant heifers (P<0.05). In contrast, in the blood plasma of the late-pregnant heifers, we did not find an increase in the isoflavone metabolite concentrations compared with the early-pregnant heifers (P>0.05). In conclusion, physiological status (cyclicity or pregnancy) of the females influenced the concentrations of isoflavone metabolites in the blood plasma of the heifers. The stage of pregnancy affects isoflavone absorption, biotransformation and metabolism differently and results in higher concentrations of active metabolites of isoflavones during early pregnancy in comparison to their lower concentrations during late pregnancy. Therefore, we surmise that cows are more sensitive to active isoflavone metabolite actions during early pregnancy than cyclic heifers and heifers in late pregnancy. Key words: Cow, Estrous cycle, Isoflavone, Phytoestrogen, Pregnancy (J. Reprod. Dev. 54: [358][359][360][361][362][363] 2008) ost of the fodder commonly used for feeding ruminants has been reported to contain phytoestrogens. In particular, alfalfa, red clover and soy bean contain 5 to even 25% phytoestrogens, such as coumestrol, genistein, daidzein, formonentin and biochanin A [1][2][3]. Red clover silage containing isoflavones [1], alfalfa containing coumestrans [4] as well as soy bean containing up to 25% of daidzein and genistein have been reported to cause infertility in cattle. The role of phytoestrogens in causing infertility is mainly recognized by accompanying signs of estrogenism including mammary development, swelling of the vulva and enlargement of the uterus [4]. Many cows suffer behavioral abnormalities such as irregular estrus, nymphomania and even anestrus [5]. Cows fail to conceive from servicing. These data are in agreement with our previous studies showing that long term feeding of a soy diet significantly increases the mean insemination rate and causes infertility in cattle [6], modulates prostaglandin secretion and action [7,8] and inhibits LH-stimulated progesterone (P4) secret...

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Soybean-Derived Phytoestrogens Regulate Prostaglandin Secretion in Endometrium During Cattle Estrous Cycle and Early Pregnancy

Cited by 76 publications

References 59 publications

Lysophosphatidic Acid Action During Early Pregnancy in the Cow: In Vivo and In Vitro Studies

Lysophosphatidic Acid Action During Early Pregnancy in the Cow: In Vivo and In Vitro Studies

Effects of dietary meals containing different levels of phytoestrogens on reproductive function in Bilgoraj ganders

Concentrations of Isoflavones and Their Metabolites in the Blood of Pregnant and Non-pregnant Heifers Fed Soy Bean

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