In the low earth orbit environment, many environmental factors lead to the degradation of material properties. The synergistic effect of long-term atomic oxygen (AO) irradiation and instantaneous impact of micro debris (MD) on long-term and transient space environmental factors has attracted more and more attention. In this paper, the performance evolution of Kapton films under the conditions of MD, AO single factor load spectrum and MD + AO, AO + MD asynchronous synergistic load spectrum were studied by laser driven flyer and microwave atomic oxygen technology. The macro morphology, optical properties and quality changes of Kapton films before and after each load spectrum were compared, and the mechanism of micro morphology and structure changes was explored. The results show that compared with MD + AO loading spectrum, the surface holes of Kapton films are larger under AO + MD load spectrum condition, the residual aluminum particles formed by reverse sputtering of Al particles during impact are less, the average transmittance of the film decreases slightly, and the weight loss of Kapton film is slightly more under the same atomic oxygen exposure time. Under the condition of MD + AO load spectrum, plastic tearing cracks, craters and holes are formed on the surface of Kapton film; the edge of the hole formed under the condition of AO + MD load spectrum is straight, without obvious depression and tear characteristics. Under the condition of MD + AO load spectrum, due to the adhesion of Al after the impact of micro debris, the subsequent atomic oxygen erosion of the film is reduced, so the C-C bond is not seriously damaged, and a considerable part of the residual aluminum flyer is oxidized to alumina by atomic oxygen; The AO + MD loading spectrum test makes the film first eroded by atomic oxygen, resulting in the reduction in C–O bond and C–C bond. The fracture of C–N bond is caused by the hypervelocity impact of micro debris. Hypervelocity impact leads to the thermal decomposition of the material, destroys the C–N bond in the imide ring and generates an N–H bond. This study will provide a method reference and a reference for the multi-factor ground collaborative simulation of space environment of spacecraft materials.