Spatial analysis of epidemics of Grapevine leafroll associated virus-3

Cabaleiro, C.; Couceiro, Carolina; Pereira, Sônia Regina; Cid, Miguel; Barrasa, Martín; Segura, Antonio

doi:10.1007/s10658-007-9254-1

Cited by 50 publications

(44 citation statements)

References 17 publications

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“…These phytophagous insect vectors do not represent a direct problem for the local viticulture industry, and therefore control measures are usually not taken. However there is evidence of Planococcus citri presence in vineyards of the Balearic Islands (Conselleria d'Agricultura i Pesca, Govern de Les Illes Balears, personal communication), and this insect is a well-known vector of GLRaV-3 (Cabaleiro et al, 2008). The combination of a long history of viticulture, the presence of insect vectors, and the lack of sanitary stocks have possibly contributed to a high incidence of viral infection.…”

Section: Virus Incidencementioning

confidence: 99%

Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Cretazzo

Tomás

Padilla³

et al. 1970

Span. j. agric. res.

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Three autochthonous grapevine varieties of Majorca (Spain) were analyzed for the presence of viruses listed by the international certification programs. Enzyme-Linked Immuno-Sorbent Assay (ELISA) screenings were performed in 193 vines from 46 vineyards included in a clonal selection. Virus-free vines were only 6.4%, 9.6% and 11.5%, in Manto Negro, Callet and Moll, respectively. Infections by grapevine leafroll associated viruses (GLRaVs) were ascertained in 71%, 78% and 60% of Manto Negro, Callet and Moll vines, respectively. Each variety was also highly infected by Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV). The percentage of plants displaying multiple infections was 58.4% in Manto Negro, 63.8% in Callet and 42.6% in Moll. Thus, it was very difficult to identify virusfree clones with suitable agronomic characteristics to be considered as a reference for the grape market. In order to obtain certified propagation material under such conditions of endemic viral infection, sanitation should be the main focus in clonal selection processes. However, the time and financial requirements for proper sanitation process bring to consideration the need to use, at least temporarily, standard multiplication material while certified clones are achieved.Additional key words: autochthonous varieties, grapevine certification, standard material virus incidence. Resumen Incidencia de las infecciones víricas en antiguos viñedos de tres variedades de vid autóctonas de Mallorca: consecuencias sobre las estrategias de la selección clonalEn este estudio se analizó la presencia de las virosis contempladas por las leyes internacionales en tema de certificación en tres variedades de vid autóctonas de Mallorca. Para ello se realizó el test ELISA (enzyme-linked immunosorbent assay) sobre 193 cepas procedentes de 46 viñedos incluidos en un programa de selección clonal. Las cepas que resultaron libres de los virus analizados fueron el 6,4% en Manto Negro, el 9,6% en Callet y el 11,5% en Moll. Los porcentajes de cepas infectadas por los virus asociados al síndrome del enrollado de la vid (GLRaVs) fueron 71% en Manto Negro, 78% en Callet y 60% en Moll. Se detectaron también altas tasas de infecciones para el virus del entrenudo corto infeccioso de la vid (GFLV) y el virus del jaspeado de la vid (GFkV) en las tres variedades. Los porcentajes de cepas sujetas a infecciones múltiples fueron 58,4% en Manto Negro, 63,8% en Callet y 42,6% en Moll. Por tanto fue difícil encontrar cepas que cumpliesen tanto los requisitos sanitarios para la certificación como aquellos agronómicos necesarios según las exigencias del mercado vitivinícola. En tales condiciones de infecciones víricas endémicas, el saneamiento debería ser el primer objetivo a seguir con el fin de obtener material de propagación certificado. Sin embargo, el tiempo y los recursos económicos necesarios para llevar a cabo el proceso de saneamiento conllevan la necesidad de considerar el uso temporal de material de propagación estándar hasta que se obtengan clones cer...

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Section: Virus Incidencementioning

confidence: 99%

Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Cretazzo

Tomás

Padilla³

et al. 1970

Span. j. agric. res.

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“…Sangiovese plants, considering viruses included in the European Commission directive 2005/43/EC. Leafroll disease is an economically harmful graft-transmissible disease of grapevines and occurs in all grapevine-growing countries (Cabaleiro et al 2008). An analogous procedure was carried out to obtain in vitro Nicotiana tabacum cv.…”

Section: Plant Materialsmentioning

confidence: 99%

Modulation of viral infection in plants by exogenous guanosine

et al. 2015

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To evaluate the role of purines in antiviral treatments in plants, ribavirin (RB) and tiazofurin (TZ) were applied in combination with guanosine (GS) or adenosine (AS) in in vitro grapevine or tobacco explants infected by Grapevine leafroll associated virus 3 (GLRaV-3) and Cucumber mosaic virus (CMV), respectively. Using a microelectrochemical (trans-plasma membrane electron transport, t-PMET) technique, in vivo assay of free reduced nicotinamide adenine dinucleotide (NADH) was also carried out to estimate the inosine monophosphate dehydrogenase inhibition caused by drugs. Antiviral effectiveness of TZ, evaluated as virus-free explants or virus copies, was significantly hindered by GS in both species, while AS did not interfere with the drugs. GS, but not AS, slightly hindered the antiviral effectiveness of RB. With regard to NADH tests, t-PMET inhibition caused by RB and TZ was dose dependent and the interference of drugs with the NAD ? /NADH conversion was confirmed by NADH content. Findings indicate that exogenous GS up to 0.50 mM replenished the GS pool depleted by drugs, contrasting antiviral action. At higher doses of GS, the TZ antiviral action was completely inhibited and exogenous GS caused a feedback that reduced t-PMET activity. The reversal was partially against RB, suggesting that the reduction of the GS pool contributed to the antiviral activity of RB, but it was not the only cause of antiviral effectiveness.

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“…Globally, five serologically distinct, phloem limited viruses designated Grapevine Leafroll-associated viruses (GLRaV) 1-4 and 7, are associated with GLD (Fuchs et al, 2009a;Martelli et al, 2012Martelli et al, , 2002 and of these, GLRaV-3 is the most common (Cabaleiro et al, 2007). GLRaV-3 is part of the Closteroviridae family, where it is type species for the Ampelovirus (Martelli et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcription loop-mediated amplification method

Walsh

Pietersen

2013

Journal of Virological Methods

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Spatial analysis of epidemics of Grapevine leafroll associated virus-3

Abstract: Several vineyards in Rías Baixas and one in the Ribeira Sacra (Spain) were monitored and the spatial pattern of leafroll-diseased grapevine was analysed at several dates. Unidimensional aggregation analysis (ordinary runs)

Cited by 50 publications

References 17 publications

Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Modulation of viral infection in plants by exogenous guanosine

Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcription loop-mediated amplification method

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