The spatial-temporal evolution of muscle tissue sample plasma induced by a high-power transversely excited atmospheric (TEA) CO 2 pulsed laser at vacuum conditions (0.1-0.01 Pa) has been investigated using high-resolution optical emission spectroscopy (OES) and imaging methods.The induced plasma shows mainly electronically excited neutral Na, K, C, Mg, H, Ca, N and O Plasma parameters such as electron density and temperature were measured from the spatiotemporal analysis of different species. Average velocities of some plasma species were estimated.