Spatial correlation of mouse photoreceptor-RPE thickness between SD-OCT and histology

Knott, Eric J.; Sheets, Kristopher G.; Zhou, Youwen; Gordon, William C.; Bazán, Nicolás G.

doi:10.1016/j.exer.2010.10.009

Cited by 44 publications

(35 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using a combination of imaging modalities, we have shown that there are previously unknown events materializing in BALB/c mice that can link genetic variation (Danciger et al, 2000; Ginsberg, 1985; LaVail et al, 1987b; Wenzel et al, 2001) and visual cycle dysfunction (Danciger et al, 2000) to retinal dystrophy, a hallmark characteristic of this model (LaVail et al, 1987b). In past studies, data on retinal dystrophy has typically been performed via histology and light microscopy ((LaVail et al, 1987b; Santos et al, 2010; Knott et al, 2011). This study demonstrates that this information can also be obtained by employing complementary imaging tools to provide immediate feedback on retinal status and integrity from study subjects in vivo .…”

Section: Discussionmentioning

confidence: 99%

The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging

Bell

Kaul

Bonilha

et al. 2015

Experimental Eye Research

View full text Add to dashboard Cite

BALB/cJ mice housed under normal vivarium lighting conditions can exhibit profound retinal abnormalities, including retinal infoldings, autofluorescent inflammatory cells, and photoreceptor degeneration. To explore the sensitivity of the outer retina to cyclic lighting during aging, a cohort of BALB/cJ mice was evaluated with Scanning Laser Ophthalmoscopy (SLO), Spectral-Domain Optical Coherence Tomography (OCT) and conventional histopathology. Mice were bred and reared in a low-illuminance (extracage/intracage: 13 lx/1 lx) vivarium under cyclic light (14 h light: 10 h dark). Retinal imaging (around postnatal day 70) was performed to screen for any pre-existing abnormalities and to establish a baseline. Mice with normal retinas were separated into groups (A, B, C) and placed on bottom (Groups A & B) or top (Group C) of the cage racks where cage illumination was <10 & 150 lx respectively. Experimental groups B & C were imaged multiple times over a 17 month period. Mice from group A (controls) were imaged only once post-baseline at various times for comparison to groups B & C. Mice were assessed by histology at 8, 15, 20, 36, and 56 weeks and immunohistochemistry at 15 weeks post-baseline. SLO and OCT retinal images were measured and the resulting trends displayed as a function of age and light exposure. Retinal lesions (RL) and autofluorescent foci (AFF) were identified with histology as photoreceptor layer infoldings (IF) and localized microglia/macrophages (MM), respectively. Few RL and AFF were evident at baseline. Retinal infoldings were the earliest changes followed by subjacent punctate autofluorescent MM. The colocalization of IF and MM suggests a causal relationship. The incidence of these pathological features increased in all groups relative to baseline. OCT imaging revealed thinning of the outer nuclear layer (ONL) in all groups at 1 year relative to baseline. ONL thinning followed an exponential rate of change but the decay constant varied depending on intensity of illumination of the groups. Advanced age and top row illuminance conditions resulted in significant photoreceptor cell loss as judged by decreased thickness of the ONL. Photoreceptor loss was preceded by both retinal infoldings and the presence of autofluorescent inflammatory cells in the outer retina, suggesting that these changes are early indicators of light toxicity in the BALB/cJ mouse.

show abstract

Section: Discussionmentioning

confidence: 99%

The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging

Bell

Kaul

Bonilha

et al. 2015

Experimental Eye Research

View full text Add to dashboard Cite

show abstract

“…Spectral domain optical coherence tomography (SD-OCT) is commonly used clinically to observe morphological changes in the retina and this technique has also been used to investigate numerous experimental models of injury and disease(41, 42). Multiple studies have demonstrated a strong correlation between retinal thickness measurements using SD-OCT data and histology in rodents(43, 44). In this study, we used non-invasive multimodal imaging and morphologic analysis to quantitate changes in the retinal ultrastructure and neurons within the ganglion cell layer (GCL) of an inducible type 1 diabetic rat model under the influence of systemic nicotine exposure.…”

Section: Introductionmentioning

confidence: 99%

Nicotine Accelerates Diabetes-Induced Retinal Changes

Boretsky¹,

Gupta

Tirgan

et al. 2014

Current Eye Research

View full text Add to dashboard Cite

Purpose: To investigate the effects of nicotine on retinal alterations in early stage diabetes in an established rodent model. Materials and Methods: Sprague-Dawley rats were examined using a combination of confocal scanning laser ophthalmoscopy and spectral domain optical coherence tomography to determine changes in retinal structure in response to nicotine exposure, diabetes and the combined effects of nicotine and diabetes. Diabetes was induced by a single injection of 65 mg/kg streptozotocin and nicotine injections were administered subcutaneously daily. Retinal thickness in the superior, inferior, nasal and temporal quadrants were determined based on SD-OCT volume scans (20° × 20°) centered on the optic disc. Segmentation of discrete retinal layers was performed on a subset of SD-OCT cross-sections to further examine changes in each treatment group. Survival of neurons within the ganglion cell layer (GCL) was assessed by confocal morphometric imaging. Results: The control group did not experience any significant change throughout the study. The nicotine treatment group experienced an average decrease in total retinal thickness (TRT) of 9.4 μm with the majority of the loss localized within the outer nuclear layer (ONL) as determined by segmentation analysis (P < 0.05). The diabetic group exhibited a trend toward decreased TRT while segmentation analysis of the DR group revealed significant thinning within the ONL (P < 0.05). The combination of nicotine and diabetes revealed a significant increase of 8.9 μm in the TRT (P < 0.05) accompanied by a decrease in the number of GCL neurons. Conclusions: We demonstrated significant temporal changes in retinal morphology in response to nicotine exposure, diabetes and with the combined effects of nicotine and diabetes. These findings may have implications in determining treatment strategies for diabetic patients using products containing nicotine such as cigarettes, smokeless tobacco, electronic cigarettes, or smoking cessation products.

show abstract

“…Each Heidelberg Spectralis OCT scan was imported into ImageJ Software 17 as a raw .vol file via the Open Hyex Raw plugin 17 . A median filter was then applied to all 97 slices to reduce signal noise, and the file was converted into individual 16 bit TIFF images.…”

Section: Methodsmentioning

confidence: 99%

Algorithm for the Measure of Vitreous Hyperreflective Foci in Optical Coherence Tomographic Scans of Patients With Diabetic Macular Edema

et al. 2016

View full text Add to dashboard Cite

Spatial correlation of mouse photoreceptor-RPE thickness between SD-OCT and histology

Cited by 44 publications

References 13 publications

The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging

The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging

Nicotine Accelerates Diabetes-Induced Retinal Changes

Algorithm for the Measure of Vitreous Hyperreflective Foci in Optical Coherence Tomographic Scans of Patients With Diabetic Macular Edema

Contact Info

Product

Resources

About