Spatial Distribution of Lipid Headgroups and Water Molecules at Membrane/Water Interfaces Visualized by Three-Dimensional Scanning Force Microscopy

Asakawa, Hitoshi; Yoshioka, Shunsuke; Nishimura, Kazunari; Fukuma, Takeshi

doi:10.1021/nn303229j

Cited by 86 publications

(102 citation statements)

References 43 publications

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“…All the 3D F images reported so far were obtained in an electrolyte solution [15,16,37], and there is clear evidence of an improvement in contrast compared to pure water-in fact obtaining stable resolution at all in pure water is very challenging [20]. Here, we overcome this difficulty by using an ultrasmall cantilever in a 3D scanning force microscope (3D-SFM) [38].…”

Section: Published By the American Physical Society Under The Terms Omentioning

confidence: 99%

Mechanism of atomic force microscopy imaging of three-dimensional hydration structures at a solid-liquid interface

et al. 2015

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Here we present both subnanometer imaging of three-dimensional (3D) hydration structures using atomic force microscopy (AFM) and molecular dynamics simulations of the calcite-water interface. In AFM, by scanning the 3D interfacial space in pure water and recording the force on the tip, a 3D force image can be produced, which can then be directly compared to the simulated 3D water density and forces on a model tip. Analyzing in depth the resemblance between experiment and simulation as a function of the tip-sample distance allowed us to clarify the contrast mechanism in the force images and the reason for their agreement with water density distributions. This work aims to form the theoretical basis for AFM imaging of hydration structures and enables its application to future studies on important interfacial processes at the molecular scale.

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Section: Published By the American Physical Society Under The Terms Omentioning

confidence: 99%

Mechanism of atomic force microscopy imaging of three-dimensional hydration structures at a solid-liquid interface

et al. 2015

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“…The result shows the possibility of direct imaging of 3D hydration structures with subnanometer-scale resolution. In addition, we applied this technique to investigate a lipid-water interface that has a much softer interfacial structures [26]. The obtained result suggested the possibility of 3D imaging flexible surface structures such as lipid headgroups.…”

Section: D Imagingmentioning

confidence: 94%

“…We have investigated the lipid-water interface by 3D-SFM [26]. We measured a 3D force distribution at the interface between fluctuating lipid headgroups and HEPES buffer solution ( Fig.…”

Section: Lipid-water Interfacementioning

confidence: 99%

“…Since 2010, FM-AFM has been used for visualizing vertical-2D or 3D force distributions at various solid-liquid interfaces [23,[25][26][27][28][29][30][31][32][33][34][35]. For interfaces between water and a rigid inorganic crystal, atomistic molecular dynamics (MD) simulation studies have been started towards detailed understanding of the imaging mechanism [?, [36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

“…For interfaces between water and a rigid inorganic crystal, atomistic molecular dynamics (MD) simulation studies have been started towards detailed understanding of the imaging mechanism [?, [36][37][38][39][40]. In the meanwhile, 3D measurements at interfaces between water and soft materials such as lipid bilayers [26] suggested another possibility of this technique. At a soft interface, not only solvent molecules but also surface structures are thermally fluctuating.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Advanced Instrumentation of Frequency Modulation AFM for Subnanometer-Scale 2D/3D Measurements at Solid-Liquid Interfaces

Fukuma

2015

Noncontact Atomic Force Microscopy

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Since the first demonstration of true atomic-resolution imaging by frequency modulation atomic force microscopy (FM-AFM) in liquid, the method has been used for imaging subnanometer-scale structures of various materials including minerals, biological systems and other organic molecules. Rencetly, there have been further advancements in the FM-AFM instrumentation. Three-dimensional (3D) force measurement techniques are proposed for visualizing 3D hydration structures formed at a solid-liquid interface. These methods further enabled to visualize 3D distributions of flexible surface structures at interfaces between soft materials and water. Furthermore, the fundamental performance such as force sensitivity and operation speed have been significantly improved using a small cantilever and highspeed phase detector. These technical advancements enabled direct visualization of atomic-scale interfacial phenomena at 1 frame/sec. In this chapter, these recent advancements in the FM-AFM instrumentation and their applications to the studies on various interfacial phenomena are presented.

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Hydrophilicity and Microsolvation of an Organic Molecule Resolved on the Sub‐molecular Level by Scanning Tunneling Microscopy

et al. 2018

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Low-temperature scanning tunneling microscopy was used to follow the formation of asolvation shell around an adsorbed functionalizeda zo dye from the attachment of the first water molecule to af ully solvated molecule.S pecific functional groups bind initially one water molecule each, which act as anchor points for additional water molecules. Further water attachment occurs in areas close to these functional groups even when the functional groups themselves are already saturated. In contrast, water molecules surround the hydrophobic parts of the molecule only when the twodimensional solvation shell closes around them. This study thus traces hydrophilic and hydrophobic properties of an organic molecule down to asub-molecular length scale.

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Spatial Distribution of Lipid Headgroups and Water Molecules at Membrane/Water Interfaces Visualized by Three-Dimensional Scanning Force Microscopy

Cited by 86 publications

References 43 publications

Mechanism of atomic force microscopy imaging of three-dimensional hydration structures at a solid-liquid interface

Mechanism of atomic force microscopy imaging of three-dimensional hydration structures at a solid-liquid interface

Advanced Instrumentation of Frequency Modulation AFM for Subnanometer-Scale 2D/3D Measurements at Solid-Liquid Interfaces

Hydrophilicity and Microsolvation of an Organic Molecule Resolved on the Sub‐molecular Level by Scanning Tunneling Microscopy

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