Spatial Distribution of mRNAs for Myelin Proteins in Primary Cultures of Mouse Brain

Amur‐Umarjee, Shashi; Hall, Lyra R.M.; Campagnoni, Anthony T.

doi:10.1159/000111855

Cited by 54 publications

(28 citation statements)

References 35 publications

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“…This is in contrast to axons in which it is suggested that ribosomes are excluded by the absence of minus-end distal microtubules (Baas et al, 1988). MBP mRNA is known to be present in oligodendrocyte processes (Amur-Umarjee et al, 1990), where it is translocated in the form of granules that associate with microtubules (Ainger et al, 1993). It has been suggested that the sustained directional movement of granules in oligodendrocyte processes is mediated by kinesin (Ainger et al, 1993), a plus-end-directed motor, although there is no evidence to support this hypothesis.…”

mentioning

confidence: 69%

“…Mature oligodendrocytes do not contain intermediate filaments, although oligodendrocyte progenitors have been shown to contain vimentin (Raff et al, 1984). A number of authors have noted a relationship between cytoskeletal elements and myelin constituents (Dyer and Benjamins, 1989;Gillespie et al, 1989;Wilson and Brophy, 1989), and microtubules also seem to play a role in the spatial segregation of myelin protein mRNAs in the oligodendrocyte (Colman et al, 1982;Trapp et al, 1987;Amur-Umarjee et al, 1990).…”

Section: The Role Of Microtubules In the Biology Of The Oligodendrocytementioning

confidence: 99%

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Microtubule Organization and Stability in the Oligodendrocyte

Lunn¹,

Baas

Duncan³

1997

J. Neurosci.

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The oligodendrocyte is the glial cell responsible for the formation and maintenance of CNS myelin. Because the development of neuronal morphology is known to depend on the presence of highly organized microtubule arrays, it may be hypothesized that the properties of microtubules influence the form and function of oligodendrocytes. The goals of the present study were to define the physical attributes of microtubules in oligodendrocytes maintained in vitro. The results of electron and confocal microscopy indicate that microtubules are present throughout the cell bodies and large and small processes of oligodendrocytes and are rarely associated with discrete microtubule-organizing centers. A modified "hooking" protocol demonstrated that the polarity orientation of microtubules is uniformly plus-end distal in small oligodendrocyte processes, compared with a nonuniform, predominantly plus-end distal orientation in large processes. Oligodendrocytes were exposed to the microtubule-depolymerizing drug nocodazole to examine microtubule stability in these cells. The results suggest that oligodendrocyte microtubules can be resolved into at least three distinct microtubule populations that differ in their kinetics of depolymerization in the presence of nocodazole. These findings suggest that the properties of the oligodendrocyte microtubule array reflect the functions of the different regions of this highly specialized cell.

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mentioning

confidence: 69%

Section: The Role Of Microtubules In the Biology Of The Oligodendrocytementioning

confidence: 99%

Microtubule Organization and Stability in the Oligodendrocyte

Lunn¹,

Baas

Duncan³

1997

J. Neurosci.

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“…6b). The intracellular localization of MBP polypeptides appears to be an event which is distinct from the transcriptional or transla tional expression of the gene (see accompa nying paper [33]). After about 16 DIV, some of the cells had processes that terminated in large membranous expansions ( fig.…”

Section: Distribution Pattern O F Various Myelin Proteins In Oligodenmentioning

confidence: 99%

Temporal Expression of Myelin-Specific Components in Neonatal Mouse Brain Cultures: Evidence that 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Appears Prior to Galactocerebroside

Amur‐Umarjee¹,

Dasu²,

Campagnoni³

1990

Dev Neurosci

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A range of antibodies to cell-specific markers was employed to characterize and quantify the cell types present in neonatal mouse brain cell cultures. Astrocytes were the major cell type to develop in culture and formed a bed layer by 5–7 days in vitro (DIV). A population of cells appeared on this layer which consisted of oligodendrocytes, progenitor cells and microglia. Some neurons were detected in the cultures up to at least 27 DIV. With time, the number of progenitor cells decreased in the cultures and there was a concomitant increase in the number of oligodendrocytes. Maximal numbers of cells expressing galactocerebroside (GC), 2'',3''-cyclic nucleotide 3''-phosphodiesterase (CNP) and myelin-associated glycoprotein were observed at 18 DIV. The highest number of cells expressing myelin basic protein and proteolipid protein were observed at 25–30 and 35–40 DIV, respectively. Double-label studies with antibodies to A2B5/CNP and A2B5/GC showed that several A2B5⁺, CNP⁺ cells were present at 6 DIV. In contrast, no A2B5⁺, GC⁺ cells could be seen at this age. Confirming these findings, some CNP⁺.GC^– cells were observed when cells were double-labeled with antibodies to CNP and GC. These findings suggested that the expression of CNP precedes that of GC in oligodendrocytes in these cultures.

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“…The cytoskeleton and microtubules have been shown to participate in protein translation and in the transport processes of mRNAs [Hamill et al, 1994]. Clear evidences of a link between microtubules and the mRNA of MBP, which is present in granules in close relationship with these cytoskeletal elements have been presented [Amur-Umarjee et al, 1990;Barbarese, 1995;Ainger et al, 1993].…”

Section: Introductionmentioning

confidence: 99%

Changes in the Expression of Cytoskeletal Proteins in the CNS of Transferrin Transgenic Mice

et al. 2002

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Apotransferrin injected intracranially into young rats has been shown in our laboratories to induce an early differentiation of oligodendroglial cells and an increased deposition of myelin. The expression of some myelin-specific proteins such as myelin basic protein (MBP) and 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and of their mRNAs were significantly increased in these animals. Also, in the cytoskeleton obtained from isolated myelin, it was found that several microtubule associated proteins (MAPs), particularly the stable tubule only peptide (STOP) and MAP 1B, as well as actin and tubulin were markedly increased. In the present paper, we compare the changes in expression of brain and myelin cytoskeletal proteins in a newly generated transferrin transgenic mouse (Tg), overexpressing the human transferrin gene, with the results obtained in aTf-injected rats. In the myelin cytoskeletal fraction of Tg mice there was a significant increase in the expression of MBP, tubulin, tau and STOP, similarly to what was previously found in the aTf-injected rats. Immunohistochemical studies showed that a variance with what occurs in the aTf-injected model, in which the above mentioned changes were limited to the corpus callosum, in the Tg mice the changes in expression of cytoskeletal proteins were observed in the various anatomical areas studied such as cerebral cortex, brain stem and cerebellum. There was also an increased expression of neurofilaments in the Tg animals, in contrast with results obtained in aTf-injected rats, suggesting that in the Tg mice, the continuous overexpression of Tf might also induce some neuronal changes. Changes in tau, total and acetylated tubulin and MAP 1B were observed in both neurons and OLGc. The increase in STOP was more significant in OLGc while the changes in MAP2 were exclusively found in neurons.

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Spatial Distribution of mRNAs for Myelin Proteins in Primary Cultures of Mouse Brain

Cited by 54 publications

References 35 publications

Microtubule Organization and Stability in the Oligodendrocyte

Microtubule Organization and Stability in the Oligodendrocyte

Temporal Expression of Myelin-Specific Components in Neonatal Mouse Brain Cultures: Evidence that 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Appears Prior to Galactocerebroside

Changes in the Expression of Cytoskeletal Proteins in the CNS of Transferrin Transgenic Mice

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