Spatial distribution of polygalacturonase-inhibiting proteins in Arabidopsis and their expression induced by Stemphylium solani infection

Chen, Di; Zhang, Hong; Sun, Zhenglong; Jia, Honglei; Yang, Xiaoming; Si, Jing; An, Lizhe

doi:10.1016/j.gene.2012.06.085

Cited by 9 publications

(9 citation statements)

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“…This includes the down regulated protein RESPONSIVE TO DEHYDRATION 19 (RD19), a cysteine proteinase induced by desiccation and is required for resistance against the pathogenic bacterium Ralstonia solanacearum [54]. In addition, upregulated proteins include the PLAT DOMAIN PROTEIN 1 (PLAT1) a member of the stress protein family involved in mediating response to biotic and abiotic stresses [41,55], Annexin D2 protein a calcium-binding protein that is upregulated in heat stress [56] as well as Polygalacturonase inhibitor 2 (PGIP2) induced by fungal infection and is implicated in resistance to fungal pathogen [57,58].…”

Section: Discussionmentioning

confidence: 99%

Maternal environment alters dead pericarp biochemical properties of the desert annual plant Anastatica hierochuntica L.

et al. 2020

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The dead organs enclosing embryos (DOEEs) emerge as central components of the dispersal unit (DU) capable for long-term storage of active proteins and other substances that affect seed performance and fate. We studied the effect of maternal environment (salt and salt+heat) on progeny DU (dry indehiscent fruit) focusing on pericarp properties of Anastatica hierochuntica. Stressed plants displayed increased seed abortion and low level and rate of germination. Hydrated pericarps released antimicrobial factors and allelopathic substances that inhibit germination of heterologous species. Proteome analysis of dead pericarps revealed hundreds of proteins, among them nucleases, chitinases and proteins involved in reactive oxygen species detoxification and cell wall modification. Salt treatment altered the composition and level of proteins stored in the pericarp. We observed changes in protein profile released from seeds of salt-treated plants with a notable increase in a small anti-fungal protein, defensin. The levels of phytohormones including IAA, ABA and salicylic acid were reduced in dead pericarps of stressed plants. The data presented here highlighted the predominant effects of maternal environment on progeny DUs of the desert plant A. hierochuntica, particularly on pericarp properties, which in turn might affect seed performance and fate, soil fertility and consequently plant biodiversity.

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Section: Discussionmentioning

confidence: 99%

Maternal environment alters dead pericarp biochemical properties of the desert annual plant Anastatica hierochuntica L.

et al. 2020

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“…The three pepper CaPGIPs were up-regulated at different times following stimulation of the pepper leaves by P. capcisi and abiotic stresses, including salicylic acid, methyl jasmonate, abscisic acid, wounding and cold treatment ( Wang et al, 2013 ). Moreover, Arabidopsis AtPGIP1 and AtPGIP2 transcription levels were up-regulated in response to infection with S. solani ( Di et al, 2012 ). In the present study, tobacco NtPGIP expression was unchanged during the early stage of P. capsici infection and subsequently rose to peak (2.2-fold > control) after 36 h of treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Plant cell walls combat phytopathogenic fungal invasion by producing polygalacturonase-inhibiting proteins (PGIPs) that specifically inhibit fungal PGs and promote oligogalacturonides (OGs) accumulation ( Wang et al, 2013 ; Di et al, 2012 ; Hwang et al, 2010 ; Jones and Jones, 1997 ; Federici et al, 2006 ). While the mechanisms by which OGs activate a defence response remain unknown, OGs are believed to be important elicitors of plant defense responses ( Federici et al, 2006 ; D’Ovidio et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression analysis and recombinant expression of a gene encoding a polygalacturonase-inhibiting protein from tobacco, Nicotiana tabacum

Zhang

Feng

Wang

et al. 2016

Heliyon

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Polygalacturonase inhibiting proteins (PGIPs) are major defensive proteins produced by plant cell walls that play a crucial role in pathogen resistance by reducing polygalacturonase (PG) activity. In the present study, a novel PGIP gene was isolated from tobacco (Nicotiana tabacum), hereafter referred as NtPGIP. A full-length NtPGIP cDNA of 1,412 bp with a 186 bp 5′-untranslated region (UTR), and 209 bp 3′-UTR was cloned from tobacco, NtPGIP is predicted to encode a protein of 338 amino acids. The NtPGIP sequence from genomic DNA showed no introns and sequence alignments of NtPGIP’s deduced amino acid sequence showed high homology with known PGIPs from other plant species. Moreover, the putative NtPGIP protein was closely clustered with several Solanaceae PGIPs. Further, the expression profile of NtPGIP was examined in tobacco leaves following stimulation with the oomycete Phytophthora nicotianae and other stressors, including salicylic acid (SA), abscisic acid (ABA), salt, and cold treatment. The results showed that all of the treatments up-regulated the expression of NtPGIP at different times. To understand the biochemical activity of NtPGIP gene, a full-length NtPGIP cDNA sequence was subcloned into a pET28a vector and transformed into E. coli BL21 (DE3). Recombinant proteins were successfully induced by 1.0 nmol/L IPTG and the purified proteins effectively inhibited Phytophthora capsici PG activity. The results of this study suggest that NtPGIP may be a new candidate gene with properties that could be exploited in plant breeding.

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“…PGIPs are thought to play a role in pathogen defense by preventing foreign PGs from degrading the plant cell wall [53], and it is interesting that none were detected in either our representative moss or lycophyte, given that Physcomitrella and other mosses are susceptible to fungal pathogens [54]. The PMEI tree we generated only contains genes from Arabidopsis and Medicago truncatula , and might not adequately represent the diversity in this gene family.…”

Section: Discussionmentioning

confidence: 99%

“…We compiled a list of Arabidopsis genes with known and predicted pectin-related functions using TAIR and Uniprot annotations, as well as relevant literature (Additional file 1) [1,34,42,53,58-64]. In total, we used 108 genes from Arabidopsis to identify putative pectin-related gene families in the PlantTribes 2.0 database [65].…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic analysis of pectin-related gene families in Physcomitrella patensand nine other plant species yields evolutionary insights into cell walls

et al. 2014

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BackgroundPectins are acidic sugar-containing polysaccharides that are universally conserved components of the primary cell walls of plants and modulate both tip and diffuse cell growth. However, many of their specific functions and the evolution of the genes responsible for producing and modifying them are incompletely understood. The moss Physcomitrella patens is emerging as a powerful model system for the study of plant cell walls. To identify deeply conserved pectin-related genes in Physcomitrella, we generated phylogenetic trees for 16 pectin-related gene families using sequences from ten plant genomes and analyzed the evolutionary relationships within these families.ResultsContrary to our initial hypothesis that a single ancestral gene was present for each pectin-related gene family in the common ancestor of land plants, five of the 16 gene families, including homogalacturonan galacturonosyltransferases, polygalacturonases, pectin methylesterases, homogalacturonan methyltransferases, and pectate lyase-like proteins, show evidence of multiple members in the early land plant that gave rise to the mosses and vascular plants. Seven of the gene families, the UDP-rhamnose synthases, UDP-glucuronic acid epimerases, homogalacturonan galacturonosyltransferase-like proteins, β-1,4-galactan β-1,4-galactosyltransferases, rhamnogalacturonan II xylosyltransferases, and pectin acetylesterases appear to have had a single member in the common ancestor of land plants. We detected no Physcomitrella members in the xylogalacturonan xylosyltransferase, rhamnogalacturonan I arabinosyltransferase, pectin methylesterase inhibitor, or polygalacturonase inhibitor protein families.ConclusionsSeveral gene families related to the production and modification of pectins in plants appear to have multiple members that are conserved as far back as the common ancestor of mosses and vascular plants. The presence of multiple members of these families even before the divergence of other important cell wall-related genes, such as cellulose synthases, suggests a more complex role than previously suspected for pectins in the evolution of land plants. The presence of relatively small pectin-related gene families in Physcomitrella as compared to Arabidopsis makes it an attractive target for analysis of the functions of pectins in cell walls. In contrast, the absence of genes in Physcomitrella for some families suggests that certain pectin modifications, such as homogalacturonan xylosylation, arose later during land plant evolution.

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Spatial distribution of polygalacturonase-inhibiting proteins in Arabidopsis and their expression induced by Stemphylium solani infection

Cited by 9 publications

References 39 publications

Maternal environment alters dead pericarp biochemical properties of the desert annual plant Anastatica hierochuntica L.

Maternal environment alters dead pericarp biochemical properties of the desert annual plant Anastatica hierochuntica L.

Cloning, expression analysis and recombinant expression of a gene encoding a polygalacturonase-inhibiting protein from tobacco, Nicotiana tabacum

Phylogenetic analysis of pectin-related gene families in Physcomitrella patensand nine other plant species yields evolutionary insights into cell walls

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