Abstractobjectives To conduct a cross-sectional mapping analysis of HIV knowledge in Zamb ezia Province, Mozambique, and to examine spatial patterns of HIV knowledge and associated household characteristics.methods A population-based cluster survey was administered in 2010; data were analysed from 201 enumeration areas in three geographically diverse districts: Alto Mol ocu e, Morrumbala and Namacurra. We assessed HIV knowledge scores (0-9 points) using previously validated assessment tools. Using geographic information systems (GIS), we mapped hot spots of high and low HIV knowledge. Our multivariable linear regression model estimated HIV knowledge associations with distance to nearest clinic offering antiretroviral therapy, respondent age, education, household size, number of children under five, numeracy, literacy and district of residence.