Spatial selective manipulation of microbubbles by tunable surface acoustic waves

Zhou, Wei; Niu, Lili; Cai, Feiyan; Li, Fēi; Wang, Chen; Huang, Xiaowei; Wang, Jingjing; Wu, Junru; Meng, Long; Zheng, Hairong

doi:10.1063/1.4954934

Cited by 22 publications

(11 citation statements)

References 43 publications

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“…On the other hand, due to the nonuniform distribution of acoustic energy generated by the focused ultrasound transducer, the activity of the neurons is highly dependent on their position within the acoustic field limiting experimental repeatability in mechanistic studies. Recently, miniaturized interdigital transducers (IDTs) have been developed using standard microfabrication techniques to manipulate particles, actuate droplets, mix fluids, and investigate cellular bioeffects . The vibration of paired IDTs generates the surface acoustic waves (SAWs) and the pattern of the IDTs determines the shape of the acoustic field.…”

Section: Introductionmentioning

confidence: 99%

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

et al. 2018

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Ultrasound stimulation, as a novel and noninvasive technique for neuromodulation, shows a great potential in the treatment of functional brain diseases. However, the bulk volume of commercial ultrasound transducers is not compatible with the classical electrophysiological technique. Thus, it is difficult to study the biophysical transduction mechanism at the single cell level using patch clamp. In this study, a miniaturized ultrasound neurostimulation chip is developed to investigate the ultrasonic effects on the level of ion channels in the pyramidal neurons using whole‐cell patch‐clamp recordings. Any kind of streaming of molecules in water is disregarded. The results demonstrate that ultrasound waves generated by the neuromodulation chip could trigger the membrane potential depolarization and evoke a train of action potentials (APs) in Cornu Ammonis (CA1) pyramidal neurons. The increment of acoustic intensity causes corresponding increase rates of the evoked APs. Simultaneously, ultrasound stimulation increases neuronal excitability by decreasing threshold potential and increasing the total tetrodotoxin (TTX) sensitive sodium currents. Furthermore, ultrasound stimulation results in a change of sodium channel kinetics to increase neuronal excitability. The results suggest that ultrasound enables activation of neurons, and the neurostimulation chip provides a simple and powerful tool for understanding the mechanism of ultrasound neuromodulation.

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Section: Introductionmentioning

confidence: 99%

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

et al. 2018

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“…Due to energy being coupled into the fluid, the surface wave amplitude decays exponentially , and hence there is a spatial gradient in the pressure field which gives rise to momentum flux in the fluid (termed the Reynolds stress); this acts to generate a steady fluid flow or acoustic streaming . The degree of spatial variation in the sound field governs the strength of the streaming field; for example, large Reynolds stresses occur at the interface between a bubble and a solid surface, − at sharp points, , or at the edge of a vibrating membrane . In the context of SAW mixing, the Reynolds stress can be raised by increasing the attenuation of the SAW, which in turn is achieved by an increase of frequency; however, in higher attenuation regimes, the area over which the stresses are generated is reduced.…”

Section: System Principlementioning

confidence: 99%

Capacitive Sensing for Monitoring of Microfluidic Protocols Using Nanoliter Dispensing and Acoustic Mixing

et al. 2020

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The development of protocols for bio/chemical reaction requires alternate dispensing and mixing steps. Whilst most microfluidic systems use the opening of additional parts of the channel to allow the ingress of fixed volumes of fluid, this requires knowledge of the protocol before the design of the chip. Our approach of using a microfluidic valve to regulate the flow into an initially empty cavity allows for on-chip protocol development and refinement. Mixing is provided by way of surface acoustic wave excitation; this high-frequency vibration causes steady-state streaming flows. We show that capacitive sensing can be used to measure fluid levels, even if multiple fluid types are used, such that nanolitre dispensing accuracy is achieved. Also, the capacitive readout can be used to establish mixing quality and to monitor temperature fluctuations. These capabilities allow for protocols to be conducted without optical assessment, and so will allow for multiplexing, such that reactions could be conducted, simultaneously, in multiple chambers across a chip.

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“…[21][22][23] Oscillating microbubbles of different sizes show a significant difference in amplitude under single ultrasound frequency and thus affect the membrane permeability differently. [21][22][23] Oscillating microbubbles of different sizes show a significant difference in amplitude under single ultrasound frequency and thus affect the membrane permeability differently.…”

Section: Introductionmentioning

confidence: 99%

“…As the resonant frequency of the microbubbles is highly dependent on the microbubble size, few of the microbubbles oscillate at resonant frequency when the microbubbles are exposed to ultrasound stimulation with a single frequency . Oscillating microbubbles of different sizes show a significant difference in amplitude under single ultrasound frequency and thus affect the membrane permeability differently .…”

Section: Introductionmentioning

confidence: 99%

Sonoporation of Cells by a Parallel Stable Cavitation Microbubble Array

et al. 2019

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Sonoporation is a targeted drug delivery technique that employs cavitation microbubbles to generate transient pores in the cell membrane, allowing foreign substances to enter cells by passing through the pores. Due to the broad size distribution of microbubbles, cavitation events appear to be a random process, making it difficult to achieve controllable and efficient sonoporation. In this work a technique is reported using a microfluidic device that enables in parallel modulation of membrane permeability by an oscillating microbubble array. Multirectangular channels of uniform size are created at the sidewall to generate an array of monodispersed microbubbles, which oscillate with almost the same amplitude and resonant frequency, ensuring homogeneous sonoporation with high efficacy. Stable harmonic and high harmonic signals emitted by individual oscillating microbubbles are detected by a laser Doppler vibrometer, which indicates stable cavitation occurred. Under the influence of the acoustic radiation forces induced by the oscillating microbubble, single cells can be trapped at an oscillating microbubble surface. The sonoporation of single cells is directly influenced by the individual oscillating microbubble. The parallel sonoporation of multiple cells is achieved with an efficiency of 96.6 ± 1.74% at an acoustic pressure as low as 41.7 kPa.

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Spatial selective manipulation of microbubbles by tunable surface acoustic waves

Cited by 22 publications

References 43 publications

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

Capacitive Sensing for Monitoring of Microfluidic Protocols Using Nanoliter Dispensing and Acoustic Mixing

Sonoporation of Cells by a Parallel Stable Cavitation Microbubble Array

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