Spatial sterol metabolism unveiled by stimulated Raman imaging

Zhang, Yongqing; Zhou, Yihui; Wen, Fang; Zhu, Hanlin; Ye, Cunqi; Zhang, De‐Long; Lee, Hyeon Jeong

doi:10.3389/fchem.2023.1166313

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…Finally, the MDA-MB-231 cells were washed with PBS and immersed in PBS for SRS imaging. SRS imaging was performed on a picosecond SRS microscope [16] . An ultrafast laser system with dual output at 80 MHz (picoEmerald; Applied Physics & Electronics, Berlin, Germany) provided both pump (tunable wavelength 700‒990 nm) and Stokes beams (fixed wavelength 1031 nm).…”

Section: Methodsmentioning

confidence: 99%

DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis

Fu,

Li,

Wang

et al. 2024

ABBS

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DPP3, a dipeptidyl peptidase, participates in a variety of pathophysiological processes. DPP3 is upregulated in cancer and might serve as a key factor in the tumorigenesis and progression of various malignancies. However, its specific role and molecular mechanism are still unknown. In this study, the expression of DPP3 in breast cancer tissues is analyzed using TCGA database. Kaplan-Meier survival analysis is performed to estimate the effect of DPP3 on the survival outcomes. To explore the biological function and mechanisms of DPP3 in breast cancer, biochemical and cell biology assays are conducted in vitro . DPP3 expresses at a higher level in breast cancer tissues than that in adjacent tissues in both TCGA database and clinical samples. Patients with high expression of DPP3 have poor survival outcomes. The proliferation and migration abilities of tumor cells with stable DPP3 knockout in breast cancer cell lines are significantly inhibited, and apoptosis is increased in vitro . GSEA analysis shows that DPP3 can affect lipid metabolism and fatty acid synthesis in tumors. Subsequent experiments show that DPP3 could stabilize FASN expression and thus promote fatty acid synthesis in tumor cells. The results of the metabolomic analysis also confirm that DPP3 can affect the content of free fatty acids. This study demonstrates that DPP3 plays a role in the reprogramming of fatty acid metabolism in tumors and is associated with poor prognosis in breast cancer patients. These findings will provide a new therapeutic target for the treatment of breast cancer.

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Section: Methodsmentioning

confidence: 99%

DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis

Fu,

Li,

Wang

et al. 2024

ABBS

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“…Through SRS imaging for sterol characteristic peak at 2870 cm −1 and lipid characteristic peak at 2850 cm −1 , spatial sterol metabolism was visualized in yeast. 83 The Raman signal at ∼3010 cm −1 corresponds to = C−H bonds, allowing visualization of unsaturated lipid distribution and quantifying lipid unsaturation degrees. Building on this, Jia et al conducted two-color SRS imaging at 2850 and 3010 cm −1 in fibrotic liver tissues, revealing a location-specific increase of unsaturated triglycerides during fibrosis.…”

Section: Multispectral Crs Imagingmentioning

confidence: 99%

“…Yoshifumi et al demonstrated multispectral SRS imaging in Euglena gracilis by reducing the number of spectral points to 4, selecting characteristic peaks at 2850 cm –1 , 2910 cm –1 , 2937 cm –1 , and 3050 cm –1 , to decompose the distribution of lipids, paramylon, chlorophyll II, and protein/nucleic acid in single cells (Figure A, B ) . Through SRS imaging for sterol characteristic peak at 2870 cm –1 and lipid characteristic peak at 2850 cm –1 , spatial sterol metabolism was visualized in yeast . The Raman signal at ∼3010 cm –1 corresponds to = C–H bonds, allowing visualization of unsaturated lipid distribution and quantifying lipid unsaturation degrees.…”

Section: Crs Imaging Strategies For Lipidsmentioning

confidence: 99%

Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism

Huang,

Xue,

Zhang

et al. 2024

Anal. Chem.

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Unveiling molecular signatures for disease classification by spectroscopic imaging

Huang,

Chen,

Zhang

et al. 2024

Optical Biopsy XXII: Toward Real-Time Spectroscopic Imaging and Diagnosis

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Non-obstructive azoospermia (NOA) is a severe form of male infertility characterized by impaired or absent sperm production in the testes. Microsurgical testicular sperm extraction (Micro TESE) is the primary treatment for NOA, but it faces challenges in differentiating between normal and abnormal seminiferous tubules based solely on morphology. To address this, our study employed stimulated Raman scattering (SRS) and second harmonic generation (SHG) microscopy to identify diagnostic features in human testicular tissues. Additionally, a deep learning-assisted diagnostic algorithm using multimodal imaging datasets demonstrated excellent performance in azoospermia diagnosis. Utilizing a weakly supervised Multiple Instance Learning-Convolutional Neuron Network (MIL-CNN) model framework, we achieved a 96% classification accuracy, surpassing the supervised CNN model. Gradient-weighted class activation mapping (Grad CAM) visualization confirmed the model’s focus on the spermatogenic region, demonstrating the potential of SRS/SHG microscopy coupled with deep learning to accurately classify normal and abnormal spermatogenic tubules, enhancing the efficiency and accuracy of pathological diagnosis.

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Spatial sterol metabolism unveiled by stimulated Raman imaging

Cited by 4 publications

References 66 publications

DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis

DPP3 promotes breast cancer tumorigenesis by stabilizing FASN and promoting lipid synthesis

Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism

Unveiling molecular signatures for disease classification by spectroscopic imaging

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