Spatially confined photoinactivation of bacteria: towards novel tools for detailed mechanistic studies

Thomsen, Hanna; James, Jeemol; Farewell, Anne; Ericson, Marica B.

doi:10.1117/12.2290718

Cited by 1 publication

(1 citation statement)

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“…. Surprisingly, few investigations have used MPM for exploring microbial biofilms so far . Two‐photon excitation (2PE) takes advantage of 2‐photon absorption, in which 2 photons of light are absorbed with lower energy than required to excite a molecule with 1 photon absorption.…”

Section: Introductionmentioning

confidence: 99%

Exploring photoinactivation of microbial biofilms using laser scanning microscopy and confined 2‐photon excitation

Thomsen

Farewell

et al. 2018

Journal of Biophotonics

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One pertinent complication in bacterial infection is the growth of biofilms, that is, communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation (PDI) has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are required. Herein, we present an approach employing multiphoton microscopy complemented with Airyscan super-resolution microscopy, to visualize the distribution of curcumin in Staphylococcus epidermidis biofilms. The effects of complexation of curcumin with hydroxypropyl-γ-cyclodextrin (HPγCD) were studied. It was shown that HPγCD curcumin demonstrated higher bioavailability in the biofilms compared to curcumin, without affecting the subcellular uptake. Spectral quantification following PDI demonstrates a method for monitoring elimination of biofilms in real time using noninvasive 3D imaging. Additionally, spatially confined 2-photon inactivation was demonstrated for the first time in biofilms. These results support the feasibility of advanced optical microscopy as a sensitive tool for evaluating treatment efficacy in biofilms toward improved mechanistic studies of PDI.

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Section: Introductionmentioning

confidence: 99%