Spatially pathogenic forms of tau detected in Alzheimer's disease brain tissue by fluorescence lifetime-based Förster resonance energy transfer

Milestones in the development and implementation of FRET-based sensors of intracellular signals: A biological perspective of the history of FRET

“…[69] FRET probes have been developed to interrogate a wide variety of processes in cellular signaling. [70,71] We can also use FRET to examine causes, treatments and potential diagnostic tools of disease [72][73][74] and in pharmaceutical studies in high throughput screening platforms for compounds or drug screening. [20,75,76] FRET is also being paired with other detection methods for faster and more accurate acquisition of information.…”

Förster resonance energy transfer microscopy and spectroscopy for localizing protein–protein interactions in living cells

“…Confocal FRET microscopy was used to track the internalization of transferrin receptor-ligand complexes in live cells (27,33). FRET imaging has also been used to investigate the causes along with potential diagnostic tools and treatments for diseases (34)(35)(36). In the Alzheimer's disease study, advanced fluorescence lifetime-based FRET imaging enabled the detection of the spatial abnormalities of tau molecules as pathogenic markers in tissue sections (36).…”

“…FRET imaging has also been used to investigate the causes along with potential diagnostic tools and treatments for diseases (34)(35)(36). In the Alzheimer's disease study, advanced fluorescence lifetime-based FRET imaging enabled the detection of the spatial abnormalities of tau molecules as pathogenic markers in tissue sections (36). In pharmaceutical research, FRET imaging is used extensively in high throughput or content screening platforms for compound or drug screening (37)(38)(39).…”